Abstract：
:Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves and artificial diets, but little is known about its concentration and redox state in midgut fluids, or the extent of Fenton-type reactions in these conditions. This study compared the levels of hydroxyl radicals and iron in the midgut fluids of two species of caterpillars: Orgyia leucostigma, in which phenol oxidation is limited, and Malacosoma disstria, in which phenol oxidation is more extensive. We tested two hypotheses: (1) higher levels of hydroxyl radicals are formed in M. disstria (consistent with the higher concentrations of hydrogen peroxide in this species), and (2) lower concentrations of iron are present in O. leucostigma (providing greater protection of its midgut fluids from oxidative damage). Hydroxyl radical levels increased greatly in M. disstria, but not in O. leucostigma, when they consumed a tannin-containing diet, supporting the first hypothesis. Protein oxidation was also significantly increased in the midgut fluids of M. disstria that ingested tannic acid, consistent with hydroxyl radical damage. Contrary to the second hypothesis, similar concentrations of iron (70 microM) remained in solution or suspension in both species of caterpillars on an artificial diet. Over 90% of this iron appeared to be in the reduced (catalytically active) state in both species. We conclude that tree-feeding caterpillars protect their midgut fluids from oxidative damage caused by Fenton-type reactions by limiting the formation of peroxides, rather than by limiting the availability of reduced iron.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Barbehenn R,Dodick T,Poopat U,Spencer Bdoi
10.1002/arch.20079subject
Has Abstractpub_date
2005-09-01 00:00:00pages
32-43issue
1eissn
0739-4462issn
1520-6327journal_volume
60pub_type
杂志文章abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20456
更新日期：2012-06-01 00:00:00
abstract：:Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期：1996-01-01 00:00:00
abstract：:Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940260207
更新日期：1994-01-01 00:00:00
abstract：:This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20412
更新日期：2011-04-01 00:00:00
abstract：:Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, ha...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.1023
更新日期：2001-03-01 00:00:00
abstract：:Nineteen 5-phenyloxazoles (5POs) were examined for their ability to modulate adenylate cyclase by measuring cAMP produced in head membrane homogenates of fifth instar larvae of the silkworm Bombyx mori. Among the compounds tested, 5-(4-methoxyphenyl)oxazole (9) and the 2,6-dichlorophenyl congener showed the highest ac...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10058
更新日期：2003-01-01 00:00:00
abstract：:The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G p...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20379
更新日期：2010-09-01 00:00:00
abstract：:The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative tra...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21739
更新日期：2020-12-01 00:00:00
abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200102
更新日期：1992-01-01 00:00:00
abstract：:The hemolymph of each noctuid species successfully parasitized by Chelonus near curvimaculatus possessed a parasitism-specific protein (PSP) previously identified in host T. ni (Insect Biochem. 19:445;21:845). Expression of PSP occurred in a stage-specific manner in the stadium during which the host undergoes precocio...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940240305
更新日期：1993-01-01 00:00:00
abstract：:Male German cockroaches possess secretory glands that secrete fluid into a pair of transverse depressions on the seventh and eighth abdominal tergites. We investigated the effects of altered juvenoid titer during the first part of the last instar on tergal gland secretory tissue development and the production of terga...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20137
更新日期：2006-09-01 00:00:00
abstract：:Immunoreactivity against peptides of the allatostatin family having a typical YXFGL-NH2 C-terminus has been localized in different areas of the central nervous system, stomatogastric nervous system and gut of the cockroach Blattella germanica. In the protocerebrum, the most characteristic immunoreactive perikarya are ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1998)37:4<269::AID-ARCH2>3
更新日期：1998-01-01 00:00:00
abstract：:Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception durin...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940300205
更新日期：1995-01-01 00:00:00
abstract：:We have used a parasitoid wasp Drosophila melanogaster system to investigate the relationship between the humoral and cellular immune responses in insects. Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was stu...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(200002)43:2<64::AID-ARCH2>
更新日期：2000-02-01 00:00:00
abstract：:The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940210402
更新日期：1992-01-01 00:00:00
abstract：:Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21332
更新日期：2016-07-01 00:00:00
abstract：:Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the obser...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10119
更新日期：2004-01-01 00:00:00
abstract：:The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20376
更新日期：2010-11-01 00:00:00
abstract：:The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21073
更新日期：2013-02-01 00:00:00
abstract：:A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was una...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21221
更新日期：2015-05-01 00:00:00
abstract：:Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight bi...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940150403
更新日期：1990-01-01 00:00:00
abstract：:The bioinsecticide Bacillus thuringiensis var. israelensis (Bti) is increasingly used worldwide for mosquito control. Although no established resistance to Bti has been described in the field so far, a resistant Aedes aegypti strain (LiTOX strain) was selected in the laboratory using field-collected leaf litter contai...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21076
更新日期：2013-02-01 00:00:00
abstract：:Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940150104
更新日期：1990-01-01 00:00:00
abstract：:The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10143
更新日期：2004-06-01 00:00:00
abstract：:Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulat...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21725
更新日期：2020-09-01 00:00:00
abstract：:Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21527
更新日期：2019-02-01 00:00:00
abstract：:In vivo treatments of female sex pheromone glands of the processionary moth, Thaumetopoea pityocampa, with mass-labeled fatty acids showed that (Z)-13-hexadecen-11-ynyl acetate, the main sex pheromone component, is biosynthesized from palmitic acid by the combined action of delta-11 and delta-13 desaturases. The invol...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940140106
更新日期：1990-01-01 00:00:00
abstract：:Bostrichiformia is the less known major series of Coleoptera regarding digestive physiology. The midgut of Dermestes maculatus has a cylindrical ventriculus with anterior caeca. There is no cell differentiation along the ventriculus, except for the predominance of cells undergoing apocrine secretion in the anterior re...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20153
更新日期：2007-01-01 00:00:00
abstract：:Intracellular activities of K+, H+, Mg2+, Ca2+, and Cl-, measured with ion selective microelectrodes in the oocyte and the nurse cells in ovarian follicles of Hyalophora cecropia, indicated that a Ca2+ current is a key component of the electrical potential that is maintained across the intercellular bridges connecting...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940250103
更新日期：1994-01-01 00:00:00
abstract：:Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20118
更新日期：2006-05-01 00:00:00
