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Molecular characterization and gene expression of apolipophorin III from the ghost moth, Thitarodes pui (Lepidoptera, Hepialidae).

Abstract：

:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 amino acid residues, the 171-residue mature apoLp-III has a calculated molecular mass of 18,606 Da. T. pui apoLp-III shares little sequence homologies (<36%) with other apoLp-IIIs. Phylogenetic analysis reveals that T. pui apoLp-III belongs to a distinct, early diverging lineage of lepidopteran apoLp-IIIs. Homology modeling of T. pui apoLp-III shows a bundle of five amphipathic α-helices, including a short helix 3'. T. pui apoLp-III was constitutively expressed in larval fat body at lower levels than pupal and adult fat body. Significant induction of apoLp-III expression, associated with strongest nodulation response, was observed in both sixth and eighth instar larvae challenged with Beauveria bassiana conidia at 1 hr after inoculation, compared with saline-injected controls. The inoculation experiment as well as previous field studies revealed the relative susceptibility of the sixth instar to the entomopathogenic fungus. ApoLp-III transcripts in the infected sixth and eighth instars were found to be induced highest 2- and 14.7-fold, respectively, during the first 12 hr. In late-stage infection, the infected susceptible sixth instar showed decrease in apoLp-III expression followed by production of B. bassiana hyphal bodies, whereas the infected eighth instar showed longer lasting increase in the expression. These results suggest that apoLp-III might contribute to T. pui immune response against fungal pathogens.

journal_name

Arch Insect Biochem Physiol

journal_title

Archives of insect biochemistry and physiology

authors

Sun Z,Yu J,Wu W,Zhang G

doi

10.1002/arch.20456

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

1-14

issue

eissn

0739-4462

issn

1520-6327

journal_volume

pub_type

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Molecular characterization and gene expression of apolipophorin III from the ghost moth, Thitarodes pui (Lepidoptera, Hepialidae).