Abstract:
:Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or within 24 h when lower concentrations were used (1 x 10(-9) M). Fractionation of an extract of Drosophila Kc167 cells combined with ligand overlay technique resulted in the identification of a putative azadirachtin binding complex. Identification of the members of this complex by Peptide Mass Fingerprinting (PMF) and N-terminal sequencing identified heat shock protein 60 (hsp60) as one of its components.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Robertson SL,Ni W,Dhadialla TS,Nisbet AJ,McCusker C,Ley SV,Mordue W,Mordue 'Luntz' AJdoi
10.1002/arch.20171subject
Has Abstractpub_date
2007-04-01 00:00:00pages
200-8issue
4eissn
0739-4462issn
1520-6327journal_volume
64pub_type
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journal_title:Archives of insect biochemistry and physiology
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