Abstract：
:Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulating protein functions in eukaryotic cells and for some mammalian proteins it is influenced by PGs. We posed the hypothesis that PGs influence protein phosphorylation within insect cells, which we tested with the established insect cell line, BCIRL-HzAM1. After 20, 30, or 40 min incubations in the presence of one of three PGs (at 15 μM), PGA2 , PGE1 , or PGF2α , separate sets of cells were processed for analysis by two-dimensional electrophoresis followed by tandem mass spectrometry. We recorded significant phosphorylation changes in 31 proteins, decreases in 15, and increases in 15, and one protein with increased or decreased phosphorylation, depending on PG treatment. Increasing PG exposure times led to changes in fewer proteins, 20 min incubations led to changes in 16 proteins, 30 min to changes in 13, and 40 min to changes in 2 proteins. The proteins were identified by bioinformatic analyses, including transcript description, calculated molecular weights and isoelectric points, MOlecular Weight SEarch score, total ion score, numbers of peptides, percent protein coverage, E-value, and highest peptide score. The data presented in this paper firmly support our hypothesis that PGs influence protein phosphorylation within insect cells and adds a novel PG-signaled function to insect biology.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Stanley D,Goodman CL,Ringbauer JA,Song Qdoi
10.1002/arch.21725subject
Has Abstractpub_date
2020-09-01 00:00:00pages
e21725issue
1eissn
0739-4462issn
1520-6327journal_volume
105pub_type
杂志文章abstract：:Acromelic acid, a naturally occurring kainoid, isolated from the mushroom Clitocybe acromelalga, is a weak displacer of [3H]L-glutamate binding to cockroach (Periplaneta americana) nerve cord membranes. Acromelic acid (1 mM) displaces approximately 60% of specifically bound [3H]L-glutamate. When applied by bath perfus...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940250202
更新日期：1994-01-01 00:00:00
abstract：:Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20171
更新日期：2007-04-01 00:00:00
abstract：:Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21527
更新日期：2019-02-01 00:00:00
abstract：:Insect γ-aminobutyric acid receptors (GABARs) are important molecular targets of cyclodiene and phenylpyrazole insecticides. Previously GABARs encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. In this study, we cl...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21232
更新日期：2015-04-01 00:00:00
abstract：:This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940190404
更新日期：1992-01-01 00:00:00
abstract：:Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-ind...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21393
更新日期：2017-08-01 00:00:00
abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20456
更新日期：2012-06-01 00:00:00
abstract：:Plants of the Asteraceae and Hypericaceae possess secondary compounds that induce photooxidation in insect herbivores that consume them. One of the well-established modes of action of these substances is peroxidation of membrane lipids. Some herbivores counteract these defences by avoidance of light and tissues rich i...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940290210
更新日期：1995-01-01 00:00:00
abstract：:The polyhydroxy structure of ecdysteroids provides a basis for the formation of various derivatives including the acetonides that have been frequently prepared mainly in preparative work. Acetonides can be formed between vicinal diols in cis position such as positions C-2/C-3 and C-20/C-22 in 20-hydroxyecdysone, for e...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1999)41:3<162::AID-ARCH8>3
更新日期：1999-01-01 00:00:00
abstract：:This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1999)40:4<203::AID-ARCH5>3
更新日期：1999-01-01 00:00:00
abstract：:Hydrocarbon and wax ester components of cuticular lipids of the braconid parasitoid Habrobracon hebetor Say reared at 25 degrees C on larvae of a pyralid moth have been identified by GC-MS and analyzed with respect to adult age, mating status, and diet. The hydrocarbons range in carbon number from C(21) to C(45) and c...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10078
更新日期：2003-05-01 00:00:00
abstract：:The larvae and adults of Aedes aegypti were tested for the potential to develop resistance to the synthetic pyrethroid, deltamethrin, alone or a combination of deltamethrin with the synergist, piperonyl butoxide (PBO). Although continuous larval selection for 40 generations resulted in 703-fold resistance, the resista...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10021
更新日期：2002-05-01 00:00:00
abstract：:Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. We purified GOX 23-fold by isoelectric focusing of labial gland homogenates and investigated physical and kinetic properties of the enzyme. D-glucose and 6-deoxy-D-glucose were the optimal substrates of...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(199909)42:1<99::AID-ARCH10
更新日期：1999-09-01 00:00:00
abstract：:Earlier work had shown that JH acts on the membrane of the follicle cell of Locusta migratoria, bringing about a rapid reduction in volume which can be detected in vitro by measuring the increase in optical path difference using quantitative interference microscopy. The juvenoid fenoxycarb, a phenoxyphenyl derivative,...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1996)32:3/4<613::AID-ARCH3
更新日期：1996-01-01 00:00:00
abstract：:Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期：1996-01-01 00:00:00
abstract：:The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20255
更新日期：2008-06-01 00:00:00
abstract：:To determine differential gene expression profiles in the venom gland and sac (gland/sac) of a solitary hunting wasp species, Orancistrocerus drewseni Saussure (1857), a subtractive cDNA library was constructed by suppression subtractive hybridization. A total of 498 expressed sequence tags (EST) were clustered and as...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20316
更新日期：2009-08-01 00:00:00
abstract：:Nineteen 5-phenyloxazoles (5POs) were examined for their ability to modulate adenylate cyclase by measuring cAMP produced in head membrane homogenates of fifth instar larvae of the silkworm Bombyx mori. Among the compounds tested, 5-(4-methoxyphenyl)oxazole (9) and the 2,6-dichlorophenyl congener showed the highest ac...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10058
更新日期：2003-01-01 00:00:00
abstract：:Salivary enzyme, glucose oxidase (GOX) from the caterpillar Helicoverpa zea, catalyzes the conversion of glucose to gluconic acid and hydrogen peroxide. Because hydrogen peroxide has well-known antimicrobial properties, we examined whether caterpillar labial saliva could reduce the infectivity of bacterial pathogens. ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20031
更新日期：2005-02-01 00:00:00
abstract：:Bt crop pyramids produce two or more Bt proteins active to broaden the spectrum of action and to delay the development of resistance in exposed insect populations. The cross-resistance between Bt toxins is a vital restriction factor for Bt crop pyramids, which may reduce the effect of pyramid strategy. In this review,...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章,评审
doi：10.1002/arch.21547
更新日期：2019-11-01 00:00:00
abstract：:The presence of allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata was confirmed by immunocytochemistry, bioassay, and HPLC. Immunocytochemical reactivity with monoclonal antibody against allatostatin I showed strong allatostatin immunoreactivity in the antennal hea...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200403
更新日期：1992-01-01 00:00:00
abstract：:RNA interference (RNAi) has become an integral part of mainstream research due to its versatility and ease of use. However, the potential nontarget effects associated with double-stranded RNAs (dsRNA) are poorly understood. To explore this, we used dsRNAs targeting the inhibitor of apoptosis (iap) gene from nine insec...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21683
更新日期：2020-08-01 00:00:00
abstract：:Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10062
更新日期：2002-12-01 00:00:00
abstract：:A neuronal morphological phenotype can be induced in cultured Spodoptera frugiperda insect cells (Sf21) by supplementing serum-containing media with 20-hydroxyecdysone (20-HE) and/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21249
更新日期：2015-11-01 00:00:00
abstract：:The biological activity of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was examined in vivo toward larvae and pupae of Galleriae mellonella L. (Lepidoptera: Pyralidae), and in vitro toward bacterial and fungal cultures, as well as cultured insect cells. Pupae of G. mellonella were far more susceptibl...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20100
更新日期：2006-02-01 00:00:00
abstract：:Juvenile hormone (JH) allows larval molting in response to ecdysteroids but prevents the switching of gene expression necessary for metamorphosis. I first review our efforts to isolate the nuclear receptor for JH in the larval epidermis of Manduca sexta using photoaffinity analogs and our recent findings that the mole...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1520-6327(1996)32:3/4<271::AID-ARCH2
更新日期：1996-01-01 00:00:00
abstract：:Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21595
更新日期：2019-09-01 00:00:00
abstract：:The hemocyte line BTI-EA-1174-A from the lepidopteran insect Estigmene acraea responds to bacterial lipopolysaccharide (LPS) by an enhanced phagocytic reaction and a dose-dependent increase of lysozyme release [Wittwer et al., Dev Comp Immunol 21:323 (1997)]. This paper provides evidence for a strong proteolytic activ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1998)39:3<91::AID-ARCH1>3.
更新日期：1998-01-01 00:00:00
abstract：:Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章,评审
doi：10.1002/arch.21652
更新日期：2020-03-01 00:00:00
abstract：:Coexistence or displacement of parasitoids in hosts during intrinsic competitive interactions between different parasitoid species (multiparasitism) may depend on their life history traits and behavior. Intense competition for possession of hosts may lead to the elimination of the inferior competitor through physical ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21321
更新日期：2016-06-01 00:00:00
