Knockdown of the ionotropic γ-aminobutyric acid receptor (GABAR) RDL gene decreases fipronil susceptibility of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae).

Abstract:

:Insect γ-aminobutyric acid receptors (GABARs) are important molecular targets of cyclodiene and phenylpyrazole insecticides. Previously GABARs encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. In this study, we cloned the open reading frame cDNA sequence of rdl gene from fipronil-susceptible and fipronil-resistant strains of Laodelphax striatellus (Lsrdl). Sequence analysis confirmed the presence of a previously identified resistance-conferring mutation. Different alternative splicing variants of Lsrdl were noted. Injection of dsLsrdl reduced the mRNA abundance of Lsrdl by 27-82%, and greatly decreased fipronil-induced mortality of individuals from both susceptible and resistant strains. These data indicate that Lsrdl encodes a functional RDL subunit that mediates susceptibility to fipronil. Additionally, temporal and spatial expression analysis showed that Lsrdl was expressed at higher levels in eggs, fifth-instar nymphs, and female adults than in third-instar and fourth-instar nymphs. Lsrdl was predominantly expressed in the heads of 2-day-old female adults. All these results provide useful background knowledge for better understanding of fipronil resistance related ionotropic GABA receptor rdl gene expressed variants and potential functional differences in insects.

authors

Wei Q,Wu SF,Niu CD,Yu HY,Dong YX,Gao CF

doi

10.1002/arch.21232

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

249-61

issue

4

eissn

0739-4462

issn

1520-6327

journal_volume

88

pub_type

杂志文章
  • Steady-state gradient in calcium ion activity across the intercellular bridges connecting oocytes and nurse cells in Hyalophora cecropia.

    abstract::Intracellular activities of K+, H+, Mg2+, Ca2+, and Cl-, measured with ion selective microelectrodes in the oocyte and the nurse cells in ovarian follicles of Hyalophora cecropia, indicated that a Ca2+ current is a key component of the electrical potential that is maintained across the intercellular bridges connecting...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940250103

    authors: Woodruff RI,Telfer WH

    更新日期:1994-01-01 00:00:00

  • Genomic organization of DHR38 gene in Drosophila: presence of Alu-like repeat in a translated exon and expression during embryonic development.

    abstract::The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1998)38:4<185::AID-ARCH4>3

    authors: Komonyi O,Mink M,Csiha J,Maróy P

    更新日期:1998-01-01 00:00:00

  • Antioxidant enzymes as defense mechanism against oxidative stress in midgut tissue and hemocytes of Bombyx mori larvae subjected to various stressors.

    abstract::In this study, larvae of silkworm Bombyx mori were subjected to low temperature, hypoxia, and viral infection to evaluate stressor-mediated oxidative stress (OS) and the induction of antioxidant enzymes (AOEs). Exposure to cold, hypoxia, and nuclear polyhedral virus for 24 h resulted in a significant increase in hydro...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21138

    authors: Micheal AS,Subramanyam MV

    更新日期:2013-12-01 00:00:00

  • Quantification of periviscerokinin-1 in the nervous system of the American cockroach, Periplaneta americana. An insect neuropeptide with unusual distribution.

    abstract::This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)40:4<203::AID-ARCH5>3

    authors: Wegener C,Predel R,Eckert M

    更新日期:1999-01-01 00:00:00

  • Seasonal changes in glycerol content and enzyme activities in overwintering larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis Walker.

    abstract::Enzymes associated with glycogen metabolism and glycerol synthesis in larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, were investigated over the winter in 2000-2001. Glycerol content was scarcely detected in September and October, rapidly increased in November and December, peaked in January, ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10024

    authors: Li YP,Ding L,Goto M

    更新日期:2002-06-01 00:00:00

  • Insecticidal and synergistic activity of dsRNAs targeting buprofezin-specific genes against the small brown planthopper, Laodelphax striatellus.

    abstract::The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative tra...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21739

    authors: Fang Y,Park MG,Choi JY,Park DH,Wang M,Kim HJ,Kim WJ,Je YH

    更新日期:2020-12-01 00:00:00

  • FLUID AND ION SECRETION BY MALPIGHIAN TUBULES OF LARVAL CHIRONOMIDS, Chironomus riparius: EFFECTS OF REARING SALINITY, TRANSPORT INHIBITORS, AND SEROTONIN.

    abstract::Larvae of Chironomus riparius respond to ion-poor and brackish water (IPW, BW) conditions by activating ion uptake mechanisms in the anal papillae and reducing ion absorption at the rectum, respectively. The role that the Malpighian tubules play in ion and osmoregulation under these conditions is not known in this spe...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21342

    authors: Zadeh-Tahmasebi M,Bui P,Donini A

    更新日期:2016-10-01 00:00:00

  • Purification and characterization of arginine kinase from the American cockroach (Periplaneta americana).

    abstract::The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10143

    authors: Brown AE,France RM,Grossman SH

    更新日期:2004-06-01 00:00:00

  • Increase in larval gut proteolytic activities and Bti resistance in the Dengue fever mosquito.

    abstract::The bioinsecticide Bacillus thuringiensis var. israelensis (Bti) is increasingly used worldwide for mosquito control. Although no established resistance to Bti has been described in the field so far, a resistant Aedes aegypti strain (LiTOX strain) was selected in the laboratory using field-collected leaf litter contai...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21076

    authors: Tetreau G,Stalinski R,David JP,Després L

    更新日期:2013-02-01 00:00:00

  • Comparison of three methods of parasitoid polydnavirus genomic DNA isolation to facilitate polydnavirus genomic sequencing.

    abstract::A major long-term goal of polydnavirus (PDV) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. As PDV viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20228

    authors: Rodríguez-Pérez MA,Beckage NE

    更新日期:2008-04-01 00:00:00

  • Pharmacological characterization of a Bombyx mori alpha-adrenergic-like octopamine receptor stably expressed in a mammalian cell line.

    abstract::Series of agonists and antagonists were examined for their actions on a Bombyx morialpha-adrenergic-like octopamine receptor (OAR) stably expressed in HEK-293 cells. The rank order of potency of the agonists was clonidine>naphazoline>tolazoline in Ca(2+) mobilization assays, and that of the antagonists was chlorpromaz...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20341

    authors: Huang J,Hamasaki T,Ozoe Y

    更新日期:2010-02-01 00:00:00

  • Comparative analysis of two biliproteins, BP1 and BP2, from haemolymph of cabbage white butterfly, Pieris rapae.

    abstract::Two blue-pigment binding proteins, BP1 and BP2, are present in larval and pupal haemolymph of cabbage white butterfly, Pieris rapae, and fluctuate in expression during development. Both BP1 and BP2 are found in pupal haemolymph in varying proportions as well as in adult haemolymph, while only small amounts of BP2 are ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20106

    authors: Choi CW,Nam KP,Seo DH,Choi JW,Lee CS,Kim HR,Yun CY

    更新日期:2006-04-01 00:00:00

  • Cloning and tissue distribution of two Na+/H+ exchangers from the Malpighian tubules of Aedes aegypti.

    abstract::A Na(+)/H(+) exchanger (NHE) on the apical membrane of mosquito Malpighian tubule (MT) cells is believed to participate in the blood-feeding mosquito's vital secretion of Na(+) and fluid. This study presents the molecular cloning, primary structure, and tissue distribution of two cDNAs encoding Aedes aegypti mosquito ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10057

    authors: Hart SJ,Knezetic JA,Petzel DH

    更新日期:2002-11-01 00:00:00

  • Identification of yolk platelet-associated hydrolases in the oocytes of Rhodnius prolixus.

    abstract::The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940210402

    authors: Nussenzveig RH,Oliveira PL,Masuda H

    更新日期:1992-01-01 00:00:00

  • Prostaglandins influence protein phosphorylation in established insect cell line.

    abstract::Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulat...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21725

    authors: Stanley D,Goodman CL,Ringbauer JA,Song Q

    更新日期:2020-09-01 00:00:00

  • Developmental disruption of Pseudoplusia includens and Heliothis virescens larvae by the calyx fluid and venom of Microplitis demolitor.

    abstract::Calyx fluid and venom from the braconid parasitoid Microplitis demolitor differentially affected the development of Pseudoplusia includens and Heliothis virescens. P. includens exhibited delays in larval development, supernumerary instars, and formed larval-pupal intermediates when injected with 0.01-0.10 wasp equival...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940180302

    authors: Strand MR,Dover BA

    更新日期:1991-01-01 00:00:00

  • Periodicity of sex pheromone biosynthesis, release and degradation in the lightbrown apple moth, Epiphyas postvittana (Walker).

    abstract::Pheromone titer in moths is a product of three processes occurring in or at the surface of the pheromone gland: biosynthesis, release, and intraglandular degradation, of pheromone. Changes in titers of sex pheromone, the fatty acyl pheromone analog (FAPA), and tetradecanoate, a pheromone biosynthetic intermediate, wer...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(200003)43:3<125::AID-ARCH4

    authors: Foster SP

    更新日期:2000-03-01 00:00:00

  • Inga laurina trypsin inhibitor (ILTI) obstructs Spodoptera frugiperda trypsins expressed during adaptive mechanisms against plant protease inhibitors.

    abstract::Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-ind...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21393

    authors: Machado SW,de Oliveira CFR,Zério NG,Parra JRP,Macedo MLR

    更新日期:2017-08-01 00:00:00

  • Serine protease from midgut of Bombus terrestris males.

    abstract::A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21075

    authors: Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík I

    更新日期:2013-03-01 00:00:00

  • Functional analysis of two chitinase genes during the pupation and eclosion stages of the beet armyworm Spodoptera exigua by RNA interference.

    abstract::Insect chitinases are a multigene family that is encoded by a rather large and diverse group of genes. The main function of chitinases is to digest the chitin contained in tissues such as the cuticles and gut lining during molting. In this study, we examined the role of a chitinase (SeChi) and a bacterial type chitina...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21018

    authors: Zhang D,Chen J,Yao Q,Pan Z,Chen J,Zhang W

    更新日期:2012-04-01 00:00:00

  • Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera.

    abstract::This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940190404

    authors: Gazit Y,Dunkelblum E,Ben-Aziz O,Altstein M

    更新日期:1992-01-01 00:00:00

  • Expression of a parasitism-specific protein in lepidopteran hosts of Chelonus sp.

    abstract::The hemolymph of each noctuid species successfully parasitized by Chelonus near curvimaculatus possessed a parasitism-specific protein (PSP) previously identified in host T. ni (Insect Biochem. 19:445;21:845). Expression of PSP occurred in a stage-specific manner in the stadium during which the host undergoes precocio...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940240305

    authors: Soldevila AI,Jones D

    更新日期:1993-01-01 00:00:00

  • Digestive proteinase activity in corn earworm (Helicoverpa zea) after molting and in response to lowered redox potential.

    abstract::Insect digestive proteinases are often strongly influenced by ambient physicochemical conditions, such as pH, ionic strength, and oxidation-reduction potential. Although the effects of the former two parameters are well documented, the influence of redox potential on catalytic rates of digestive enzymes is not well un...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/1520-6327(200008)44:4<151::AID-ARCH2>3.0.C

    authors: Johnson KS,Felton GW

    更新日期:2000-08-01 00:00:00

  • Farnesoic acid O-methyl transferase (FAMeT) isoforms: conserved traits and gene expression patterns related to caste differentiation in the stingless bee, Melipona scutellaris.

    abstract::Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20224

    authors: Vieira CU,Bonetti AM,Simões ZL,Maranhão AQ,Costa CS,Costa MC,Siquieroli AC,Nunes FM

    更新日期:2008-02-01 00:00:00

  • Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum.

    abstract::Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic activity of its tran...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20249

    authors: Shi M,Huang F,Chen YF,Meng XF,Chen XX

    更新日期:2009-01-01 00:00:00

  • CLONING AND EXPRESSING TRYPSIN MODULATING OOSTATIC FACTOR IN Chlorella desiccata TO CONTROL MOSQUITO LARVAE.

    abstract::The insect peptide hormone trypsin modulating oostatic factor (TMOF), a decapeptide that is synthesized by the mosquito ovary and controls the translation of the gut's trypsin mRNA was cloned and expressed in the marine alga Chlorella desiccata. To express Aedes aegypti TMOF gene (tmfA) in C. desiccata cells, two plas...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21306

    authors: Borovsky D,Sterner A,Powell CA

    更新日期:2016-01-01 00:00:00

  • Molecular analysis of the Methoprene-tolerant gene region of Drosophila melanogaster.

    abstract::Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception durin...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940300205

    authors: Turner C,Wilson TG

    更新日期:1995-01-01 00:00:00

  • Insect immunity: a genetic factor (hrtp) is essential for antibacterial peptide expression in Drosophila after infection by parasitoid wasps.

    abstract::We have used a parasitoid wasp Drosophila melanogaster system to investigate the relationship between the humoral and cellular immune responses in insects. Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was stu...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(200002)43:2<64::AID-ARCH2>

    authors: Benassi V,Coustau C,Carton Y

    更新日期:2000-02-01 00:00:00

  • Interactions between Spinacia oleracea and Bradysia impatiens: a role for phytoecdysteroids.

    abstract::Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10062

    authors: Schmelz EA,Grebenok RJ,Ohnmeiss TE,Bowers WS

    更新日期:2002-12-01 00:00:00

  • How do insect herbivores cope with the extreme oxidative stress of phototoxic host plants?

    abstract::Plants of the Asteraceae and Hypericaceae possess secondary compounds that induce photooxidation in insect herbivores that consume them. One of the well-established modes of action of these substances is peroxidation of membrane lipids. Some herbivores counteract these defences by avoidance of light and tissues rich i...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940290210

    authors: Aucoin R,Guillet G,Murray C,Philogène BJR,Arnason JT

    更新日期:1995-01-01 00:00:00