CLONING AND EXPRESSING TRYPSIN MODULATING OOSTATIC FACTOR IN Chlorella desiccata TO CONTROL MOSQUITO LARVAE.

Abstract:

:The insect peptide hormone trypsin modulating oostatic factor (TMOF), a decapeptide that is synthesized by the mosquito ovary and controls the translation of the gut's trypsin mRNA was cloned and expressed in the marine alga Chlorella desiccata. To express Aedes aegypti TMOF gene (tmfA) in C. desiccata cells, two plasmids (pYES2/TMOF and pYDB4-tmfA) were engineered with pKYLX71 DNA (5 Kb) carrying the cauliflower mosaic virus (CaMV) promoter 35S(2) and the kanamycin resistant gene (neo), as well as, a 8 Kb nitrate reductase gene (nit) from Chlorella vulgaris. Transforming C. desiccata with pYES2/TMOF and pYDB4-tmfA show that the engineered algal cells express TMOF (20 ± 4 μg ± SEM and 17 ± 3 μg ± SEM, respectively in 3 × 10(8) cells) and feeding the cells to mosquito larvae kill 75 and 60% of Ae. aegypti larvae in 4 days, respectively. Southern and Northern blots analyses show that tmfA integrated into the genome of C. desiccata by homologous recombination using the yeast 2 μ circle of replication and the nit in pYES2/TMOF and pYDB4-tmfA, respectively, and the transformed algal cells express tmfA transcript. Using these algal cells it will be possible in the future to control mosquito larvae in the marsh.

authors

Borovsky D,Sterner A,Powell CA

doi

10.1002/arch.21306

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

17-36

issue

1

eissn

0739-4462

issn

1520-6327

journal_volume

91

pub_type

杂志文章
  • Cuticular hydrocarbons and wax esters of the ectoparasitoid Habrobracon hebetor: Ontogenetic, reproductive, and nutritional effects.

    abstract::Hydrocarbon and wax ester components of cuticular lipids of the braconid parasitoid Habrobracon hebetor Say reared at 25 degrees C on larvae of a pyralid moth have been identified by GC-MS and analyzed with respect to adult age, mating status, and diet. The hydrocarbons range in carbon number from C(21) to C(45) and c...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10078

    authors: Howard RW,Baker JE

    更新日期:2003-05-01 00:00:00

  • The progress in insect cross-resistance among Bacillus thuringiensis toxins.

    abstract::Bt crop pyramids produce two or more Bt proteins active to broaden the spectrum of action and to delay the development of resistance in exposed insect populations. The cross-resistance between Bt toxins is a vital restriction factor for Bt crop pyramids, which may reduce the effect of pyramid strategy. In this review,...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章,评审

    doi:10.1002/arch.21547

    authors: Wei J,Zhang Y,An S

    更新日期:2019-11-01 00:00:00

  • Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line.

    abstract::Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules struct...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1997)34:3<313::AID-ARCH6>3

    authors: Hatt PJ,Liebon C,Morinière M,Oberlander H,Porcheron P

    更新日期:1997-01-01 00:00:00

  • Metabolism of hydrogen peroxide between diapause and non-diapause eggs of the silkworm, Bombyx mori during chilling at 5 degrees C.

    abstract::When diapause and non-diapause eggs of the same bivoltine strain of Bombyx mori were chilled at 5 degrees C for more than 30 days, the hatchability of diapause eggs increased while that of non-diapause eggs decreased, respectively. To investigate the relationship between effects of chilling on the hatchability and the...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20367

    authors: Zhao LC,Shi LG

    更新日期:2010-06-01 00:00:00

  • Actions of acromelic acid on nervous system L-glutamate receptors.

    abstract::Acromelic acid, a naturally occurring kainoid, isolated from the mushroom Clitocybe acromelalga, is a weak displacer of [3H]L-glutamate binding to cockroach (Periplaneta americana) nerve cord membranes. Acromelic acid (1 mM) displaces approximately 60% of specifically bound [3H]L-glutamate. When applied by bath perfus...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940250202

    authors: Sattelle DB,Sepúlveda MI,Shinozaki H,Ishida M

    更新日期:1994-01-01 00:00:00

  • Variation in the pH of experimental diets affects the performance of Lymantria dispar asiatica larvae and its gut microbiota.

    abstract::To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (cont...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21654

    authors: Zeng JY,Shi JH,Guo JX,Shi ZB,Zhang GC,Zhang J

    更新日期:2020-04-01 00:00:00

  • A novel type of hemocytes localizing melanization with high-spreading behavior in Mythimna separata.

    abstract::Lepidopteran larvae show a cellular response to invading foreign substances that are larger than hemocytes, for example, parasitoid eggs or larvae. This response is called hemocyte encapsulation and is often accompanied by phenoloxidase (PO)-catalyzed melanization. In the present study, we artificially transplanted en...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21173

    authors: Kato Y,Yoshida T,Miura K,Tanaka T,Nakamatsu Y,Ochiai M

    更新日期:2014-08-01 00:00:00

  • Applications of EcR gene switch technology in functional genomics.

    abstract::Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20193

    authors: Tavva VS,Palli SR,Dinkins RD,Collins GB

    更新日期:2007-07-01 00:00:00

  • Plasmatocyte spreading peptide does not induce Microplitis demolitor polydnavirus-infected plasmatocytes to spread on foreign surfaces.

    abstract::Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.

    authors: Strand MR,Clark KC,Gardiner EM

    更新日期:1999-01-01 00:00:00

  • Evidence that caterpillar labial saliva suppresses infectivity of potential bacterial pathogens.

    abstract::Salivary enzyme, glucose oxidase (GOX) from the caterpillar Helicoverpa zea, catalyzes the conversion of glucose to gluconic acid and hydrogen peroxide. Because hydrogen peroxide has well-known antimicrobial properties, we examined whether caterpillar labial saliva could reduce the infectivity of bacterial pathogens. ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20031

    authors: Musser RO,Kwon HS,Williams SA,White CJ,Romano MA,Holt SM,Bradbury S,Brown JK,Felton GW

    更新日期:2005-02-01 00:00:00

  • Isolation and characterization of bacteria-induced protein P4 from hemolymph of Manduca sexta.

    abstract::Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940150104

    authors: Ladendorff NE,Kanost MR

    更新日期:1990-01-01 00:00:00

  • Unique features of flight muscles mitochondria of honey bees (Apis mellifera L.).

    abstract::Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21595

    authors: Syromyatnikov MY,Gureev AP,Vitkalova IY,Starkov AA,Popov VN

    更新日期:2019-09-01 00:00:00

  • LARVAL X-RAY IRRADIATION INFLUENCES PROTEIN EXPRESSION IN PUPAE OF THE ORIENTAL FRUIT FLY, BACTROCERA DORSALIS.

    abstract::The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21330

    authors: Chang CL,Goodman CL,Ringbauer J,Geib SM,Stanley D

    更新日期:2016-07-01 00:00:00

  • Differential gene expression profiles in the venom gland/sac of Orancistrocerus drewseni (Hymenoptera: Eumenidae).

    abstract::To determine differential gene expression profiles in the venom gland and sac (gland/sac) of a solitary hunting wasp species, Orancistrocerus drewseni Saussure (1857), a subtractive cDNA library was constructed by suppression subtractive hybridization. A total of 498 expressed sequence tags (EST) were clustered and as...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20316

    authors: Baek JH,Woo TH,Kim CB,Park JH,Kim H,Lee S,Lee SH

    更新日期:2009-08-01 00:00:00

  • Characterization and partial cloning of ecdysteroid receptor from a cotton boll weevil embryonic cell line.

    abstract::A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1997)35:1/2<45::AID-ARCH5>

    authors: Dhadialla TS,Tzertzinis G

    更新日期:1997-01-01 00:00:00

  • Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae).

    abstract::This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20412

    authors: Mesquita-Rodrigues C,Saboia-Vahia L,Cuervo P,Levy CM,Honorio NA,Domont GB,de Jesus JB

    更新日期:2011-04-01 00:00:00

  • Cloning of a VLP-protein coding gene from a parasitoid wasp Venturia canescens.

    abstract::Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940260207

    authors: Theopold U,Krause E,Schmidt O

    更新日期:1994-01-01 00:00:00

  • Transcriptional expression of bursicon and novel bursicon-regulated genes in the house fly Musca domestica.

    abstract::Bursicon is a neuropeptide that regulates cuticle sclerotization (hardening and tanning) via a G protein-coupled receptor. It consists of two subunits, an alpha and a beta. In the present study, we investigated the transcriptional expression and in situ localization of bursicon alpha and beta in the central nerve syst...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20239

    authors: Wang S,An S,Song Q

    更新日期:2008-06-01 00:00:00

  • Remarkable similarity in body mass of a secondary hyperparasitoid Lysibia nana and its primary parasitoid host Cotesia glomerata emerging from cocoons of comparable size.

    abstract::Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20080

    authors: Harvey JA,Vet LE,Witjes LM,Bezemer TM

    更新日期:2006-03-01 00:00:00

  • Prostaglandins influence protein phosphorylation in established insect cell line.

    abstract::Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulat...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21725

    authors: Stanley D,Goodman CL,Ringbauer JA,Song Q

    更新日期:2020-09-01 00:00:00

  • Cloning and tissue distribution of two Na+/H+ exchangers from the Malpighian tubules of Aedes aegypti.

    abstract::A Na(+)/H(+) exchanger (NHE) on the apical membrane of mosquito Malpighian tubule (MT) cells is believed to participate in the blood-feeding mosquito's vital secretion of Na(+) and fluid. This study presents the molecular cloning, primary structure, and tissue distribution of two cDNAs encoding Aedes aegypti mosquito ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10057

    authors: Hart SJ,Knezetic JA,Petzel DH

    更新日期:2002-11-01 00:00:00

  • Positive and negative modulation of Bombyx mori adenylate cyclase by 5-phenyloxazoles: identification of octopamine and tyramine receptor agonists.

    abstract::Nineteen 5-phenyloxazoles (5POs) were examined for their ability to modulate adenylate cyclase by measuring cAMP produced in head membrane homogenates of fifth instar larvae of the silkworm Bombyx mori. Among the compounds tested, 5-(4-methoxyphenyl)oxazole (9) and the 2,6-dichlorophenyl congener showed the highest ac...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10058

    authors: Khan MA,Nakane T,Ohta H,Ozoe Y

    更新日期:2003-01-01 00:00:00

  • Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.

    abstract::Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promo...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21083

    authors: Tatsuke T,Lee JM,Kusakabe T,Iiyama K,Sezutsu H,Uchino K

    更新日期:2013-04-01 00:00:00

  • Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera.

    abstract::This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940190404

    authors: Gazit Y,Dunkelblum E,Ben-Aziz O,Altstein M

    更新日期:1992-01-01 00:00:00

  • Seasonal changes in glycerol content and enzyme activities in overwintering larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis Walker.

    abstract::Enzymes associated with glycogen metabolism and glycerol synthesis in larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, were investigated over the winter in 2000-2001. Glycerol content was scarcely detected in September and October, rapidly increased in November and December, peaked in January, ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10024

    authors: Li YP,Ding L,Goto M

    更新日期:2002-06-01 00:00:00

  • Neural inhibition of the corpus allatum in the last larval instar differs from that in adults in the blood-feeding insect, Rhodnius prolixus.

    abstract::An active corpus allatum (CA) in the blood feeding insect, Rhodnius prolixus, releases a substance that inhibits metamorphosis in larvae, and activates egg production in adults. In adults, transecting the nervus corporis cardiacum II's (NCCII), which are attached posteriorly to each protocerebral lobe, greatly increas...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(200005)44:1<1::AID-ARCH1>3

    authors: Chiang RG

    更新日期:2000-05-01 00:00:00

  • Depletion of autophagy-related genes ATG3 and ATG5 in Tenebrio molitor leads to decreased survivability against an intracellular pathogen, Listeria monocytogenes.

    abstract::Macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. Homologues of most of 36 originally discovered autophagy-related (ATG) genes in yeast have been characterized in higher eukaryotes inclu...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21212

    authors: Tindwa H,Jo YH,Patnaik BB,Noh MY,Kim DH,Kim I,Han YS,Lee YS,Lee BL,Kim NJ

    更新日期:2015-01-01 00:00:00

  • Starvation influences allatotropin gene expression and juvenile hormone titer in the female adult oriental armyworm, Mythimna separata.

    abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20255

    authors: Zhang L,Luo L,Jiang X

    更新日期:2008-06-01 00:00:00

  • Genes acting in longevity-related pathways in the endoparasitoid, Pteromalus puparum.

    abstract::Among insects, lifespans vary over a broad range, from the short-lived mayflies to the 17-year periodical cicadas. Generally, lifespans are determined by a phase in life, the reproductive lifespan, which varies among species. Numerous pathways, such as the insulin/insulin-like growth factor signaling pathway, the targ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21635

    authors: Xiong S,Yu K,Ye X,Fang Q,Deng Y,Xiao S,Yang L,Wang B,Wang F,Yan Z,Wang F,Song Q,Stanley DW,Ye G

    更新日期:2020-02-01 00:00:00

  • Biochemical mode of action and differential activity of new ecdysone agonists against mosquitoes and moths.

    abstract::THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20046

    authors: Palli SR,Tice CM,Margam VM,Clark AM

    更新日期:2005-04-01 00:00:00