Steady-state gradient in calcium ion activity across the intercellular bridges connecting oocytes and nurse cells in Hyalophora cecropia.

Abstract:

:Intracellular activities of K+, H+, Mg2+, Ca2+, and Cl-, measured with ion selective microelectrodes in the oocyte and the nurse cells in ovarian follicles of Hyalophora cecropia, indicated that a Ca2+ current is a key component of the electrical potential that is maintained across the intercellular bridges connecting these two cells. In vitellogenic follicles, Ca2+ activity averaged 650 nM in the oocyte and 190 nM in the nurse cells, whereas activities of the other ions studied differed between these cells by no more than 6%. Incubation in 200 microM ammonium vanadate caused a reversal of electrical potential from 8.3 mV, nurse cell negative, to 3.0 mV, oocyte negative, and at the same time the Ca2+ gradient was reversed: activities rose to an average 3.0 microM in the nurse cells and 1.6 microM in the oocyte, whereas transbridge ratios of the other cations remained at 0-3%. In immature follicles that had not yet initiated their transbridge potentials, Ca2+ activities averaged approximately 2 microM in both oocyte and nurse cells. The results suggest that vitellogenic follicles possess a vanadate-sensitive Ca2+ extrusion mechanism that is more powerful in the nurse cells than in the oocyte.

authors

Woodruff RI,Telfer WH

doi

10.1002/arch.940250103

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

9-20

issue

1

eissn

0739-4462

issn

1520-6327

journal_volume

25

pub_type

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