Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae.

abstract：:The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...

journal_title：Archives of insect biochemistry and physiology

authors： Brown AE,France RM,Grossman SH

更新日期：2004-06-01 00:00:00

abstract：:The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...

journal_title：Archives of insect biochemistry and physiology

authors： Matthews HJ,Down RE,Audsley N

更新日期：2010-11-01 00:00:00

abstract：:Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight bi...

journal_title：Archives of insect biochemistry and physiology

authors： Indrasith LS,Kindle H,Lanzrein B

更新日期：1990-01-01 00:00:00

abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...

journal_title：Archives of insect biochemistry and physiology

authors： Sun Z,Yu J,Wu W,Zhang G

更新日期：2012-06-01 00:00:00

abstract：:Molting is the result of the expression of a cascade of genes that is sequentially both up and down-regulated by the molting hormone, 20-hydroxyecdysone (20E), which is secreted as a pulse during each instar. Benzoyl hydrazine analogs of 20E act like the native molting hormone at the molecular level by binding with th...

journal_title：Archives of insect biochemistry and physiology

authors： Retnakaran A,Krell P,Feng Q,Arif B

更新日期：2003-12-01 00:00:00

abstract：:This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...

journal_title：Archives of insect biochemistry and physiology

authors： Mesquita-Rodrigues C,Saboia-Vahia L,Cuervo P,Levy CM,Honorio NA,Domont GB,de Jesus JB

更新日期：2011-04-01 00:00:00

abstract：:A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...

journal_title：Archives of insect biochemistry and physiology

authors： Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík I

更新日期：2013-03-01 00:00:00

abstract：:Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...

journal_title：Archives of insect biochemistry and physiology

authors： Aurade RM,Jayalakshmi SK,Udikeri SS,Sreeramulu K

更新日期：2012-01-01 00:00:00

abstract：:A neuronal morphological phenotype can be induced in cultured Spodoptera frugiperda insect cells (Sf21) by supplementing serum-containing media with 20-hydroxyecdysone (20-HE) and/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells...

journal_title：Archives of insect biochemistry and physiology

authors： Jenson LJ,Bloomquist JR

更新日期：2015-11-01 00:00:00

abstract：:The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male...

journal_title：Archives of insect biochemistry and physiology

authors： Chang CL,Goodman CL,Ringbauer J,Geib SM,Stanley D

更新日期：2016-07-01 00:00:00

abstract：:The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...

journal_title：Archives of insect biochemistry and physiology

authors： Nussenzveig RH,Oliveira PL,Masuda H

更新日期：1992-01-01 00:00:00

abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...

journal_title：Archives of insect biochemistry and physiology

authors： Van Antwerpen R,Law JH

更新日期：1992-01-01 00:00:00

abstract：:A cDNA clone encoding a small GTP binding protein (Brho) was isolated from an embryonic cDNA library of Bombyx mori that encoded a polypeptide with 202 amino acids sharing 60-80% similarity with the Rho1 family of GTP binding proteins. The effector site and one of the guanine nucleotide binding sites differed from oth...

journal_title：Archives of insect biochemistry and physiology

authors： Uno T,Nakasuji A,Hara W,Aizono Y

更新日期：2000-04-01 00:00:00

abstract：:Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...

journal_title：Archives of insect biochemistry and physiology

authors： Harvey JA,Vet LE,Witjes LM,Bezemer TM

更新日期：2006-03-01 00:00:00

abstract：:Alkaline phosphatase activity was histochemically localized in adult whiteflies (Bemisia tabaci B biotype, syn. B. argentifolii) with a chromogenic substrate (5-bromo-4-chloro-3-indolylphosphate) and a fluorogenic substrate (ELF-97). The greatest amount of staining was in the basal regions of adult salivary glands wit...

journal_title：Archives of insect biochemistry and physiology

authors： Funk CJ

更新日期：2001-04-01 00:00:00

abstract：:A protocol has been developed for the purification of vitellogenin from the honey bee, Apis mellifera. Purification allows for the first characterization of a vitellogenin from the large order Hymenoptera. Hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotei...

journal_title：Archives of insect biochemistry and physiology

authors： Wheeler DE,Kawooya JK

更新日期：1990-01-01 00:00:00

abstract：:This report concerns the effect of heat shock on host-pathogen interaction in Galleria mellonella infected with Bacillus thuringiensis. We show enhanced activity against Gram-positive bacteria in the hemolymph of larvae pre-exposed to heat shock before infection with B. thuringiensis. Heat shock influenced the protein...

journal_title：Archives of insect biochemistry and physiology

authors： Taszłow P,Wojda I

更新日期：2015-02-01 00:00:00

abstract：:Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promo...

journal_title：Archives of insect biochemistry and physiology

authors： Tatsuke T,Lee JM,Kusakabe T,Iiyama K,Sezutsu H,Uchino K

更新日期：2013-04-01 00:00:00

abstract：:The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...

journal_title：Archives of insect biochemistry and physiology

authors： Komonyi O,Mink M,Csiha J,Maróy P

更新日期：1998-01-01 00:00:00

abstract：:Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception durin...

journal_title：Archives of insect biochemistry and physiology

authors： Turner C,Wilson TG

更新日期：1995-01-01 00:00:00

abstract：:Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...

journal_title：Archives of insect biochemistry and physiology

authors： Syromyatnikov MY,Gureev AP,Vitkalova IY,Starkov AA,Popov VN

更新日期：2019-09-01 00:00:00

abstract：:Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...

journal_title：Archives of insect biochemistry and physiology

authors： Ladendorff NE,Kanost MR

更新日期：1990-01-01 00:00:00

abstract：:The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as t...

journal_title：Archives of insect biochemistry and physiology

authors： Girardie J,Chaabihi H,Fournier B,Lagueux M,Girardie A

更新日期：1997-01-01 00:00:00

abstract：:Aphid, a short germband insect, displays an embryogenesis different from that of long germband insect species. Furthermore, the development of its parthenogenetic and viviparous embryo is different from that of the embryo resulting from sexual reproduction. To better understand the genetic regulation of this type of e...

journal_title：Archives of insect biochemistry and physiology

authors： Mao J,Liu C,Zeng F

更新日期：2013-12-01 00:00:00

abstract：:An active corpus allatum (CA) in the blood feeding insect, Rhodnius prolixus, releases a substance that inhibits metamorphosis in larvae, and activates egg production in adults. In adults, transecting the nervus corporis cardiacum II's (NCCII), which are attached posteriorly to each protocerebral lobe, greatly increas...

journal_title：Archives of insect biochemistry and physiology

authors： Chiang RG

更新日期：2000-05-01 00:00:00

abstract：:Immunoreactivity against peptides of the allatostatin family having a typical YXFGL-NH2 C-terminus has been localized in different areas of the central nervous system, stomatogastric nervous system and gut of the cockroach Blattella germanica. In the protocerebrum, the most characteristic immunoreactive perikarya are ...

journal_title：Archives of insect biochemistry and physiology

authors： Maestro JL,Bellés X,Piulachs MD,Thorpe A,Duve H

更新日期：1998-01-01 00:00:00

abstract：:Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...

journal_title：Archives of insect biochemistry and physiology

authors： Schmelz EA,Grebenok RJ,Ohnmeiss TE,Bowers WS

更新日期：2002-12-01 00:00:00

abstract：:Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...

journal_title：Archives of insect biochemistry and physiology

authors： Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo K

更新日期：2015-09-01 00:00:00

abstract：:This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...

journal_title：Archives of insect biochemistry and physiology

authors： Wegener C,Predel R,Eckert M

更新日期：1999-01-01 00:00:00

abstract：:V-ATPases are ubiquitous proton pumps found in eukaryotes, and are important in regulating the pH of cell compartments and in creating membrane potentials. The V-ATPase creates a proton gradient that is used as an energy source for the transport of other ions. The 16-kDa proteolipid is the proton-translocating subunit...

journal_title：Archives of insect biochemistry and physiology

authors： Holmes SP,Frazier SK,Pietrantonio PV

更新日期：2000-11-01 00:00:00