Abstract：
:The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effects on mortality, growth, and fecundity compared with control insects. The analogue, δR³δR⁵Manse-AS, had an estimated LC₅₀ of 0.31 µg/µl diet and was more potent than Manse-AS (estimated LC₅₀ of 0.58 µg/µl diet). At a dose of 0.35 µg δR³δR⁵Manse-AS/µl diet, 76% of the aphids were dead after 6 days and all were dead after 10 days. In comparison, three times the dose of Manse-AS was required to achieve 74% mortality after 8 days and 98% mortality after 16 days. The degradation of both peptides by extracts prepared from the gut of M. persicae was investigated. The estimated half-life of Manse-AS, when incubated with the gut extract from M. persicae, was 31 min. Degradation was due to a cathepsin L-like cysteine protease, carboxypeptidase-like activity, endoprotease activity with glutamine specificity, pyroglutamate aminopeptidase activity, and possibly trypsin-like proteases. The half-life of the δR³δR⁵ Manse-AS analogue was enhanced (73 min) with the D-isomers of R appearing to prevent cleavage around the R residues by cathepsin L-like cysteine proteases or from trypsin-like proteases. The greater stability of the analogue may explain its increased potency in M. persicae. This work demonstrates the potential use of Manse-AS and analogues, with greater resistance to enzymatic attack, in aphid control strategies.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Matthews HJ,Down RE,Audsley Ndoi
10.1002/arch.20376subject
Has Abstractpub_date
2010-11-01 00:00:00pages
139-57issue
3eissn
0739-4462issn
1520-6327journal_volume
75pub_type
杂志文章abstract：:Pheromone titer in moths is a product of three processes occurring in or at the surface of the pheromone gland: biosynthesis, release, and intraglandular degradation, of pheromone. Changes in titers of sex pheromone, the fatty acyl pheromone analog (FAPA), and tetradecanoate, a pheromone biosynthetic intermediate, wer...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(200003)43:3<125::AID-ARCH4
更新日期：2000-03-01 00:00:00
abstract：:Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, ha...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.1023
更新日期：2001-03-01 00:00:00
abstract：:Larvae of Sesamia nonagrioides developing under long day (LD) conditions pupate in the 5th or 6th instar, whereas under the short day (SD) conditions, they undergo several supernumerary larval molts and are regarded as diapausing. The development in early larval instars occurs in the LD larvae at a moderate and in the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20181
更新日期：2007-06-01 00:00:00
abstract：:The effects of tebufenozide and methoxyfenozide on vitellogenin (Vg) synthesis/release in the fat body, translocation in hemolymph, uptake by the ovary, and the expression of the ecdysone receptor (EcR) and its heterodimer partner, ultraspiracle protein (USP) in fat body, were investigated in Cydia pomonella. The resu...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10073
更新日期：2003-03-01 00:00:00
abstract：:Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21332
更新日期：2016-07-01 00:00:00
abstract：:In this study, larvae of silkworm Bombyx mori were subjected to low temperature, hypoxia, and viral infection to evaluate stressor-mediated oxidative stress (OS) and the induction of antioxidant enzymes (AOEs). Exposure to cold, hypoxia, and nuclear polyhedral virus for 24 h resulted in a significant increase in hydro...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21138
更新日期：2013-12-01 00:00:00
abstract：:At 22°C and under a long-day photoperiod of L:D 16:8, all the last fifth instar Loxostege sticticalis larvae undergo prepupal stage and pupate without diapause. Under a short-day photoperiod of L:D 12:12, in contrast, they all enter diapause with approximately 36 days diapause maintenance and then terminate diapause s...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20429
更新日期：2011-07-01 00:00:00
abstract：:Neosurugatoxin (NSTX), a neurotoxin isolated from the Japanese ivory mollusc Babylonia japonica, is a potent neuronal nicotinic acetylcholine receptor (nAChR) antagonist. Hitherto, NSTX has been found to block only neuronal nAChRs that are insensitive to alpha-Bgt. Here, we report for the first time that NSTX blocks a...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940230403
更新日期：1993-01-01 00:00:00
abstract：:Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20171
更新日期：2007-04-01 00:00:00
abstract：:Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章,评审
doi：10.1002/arch.940290204
更新日期：1995-01-01 00:00:00
abstract：:The toxicity of potassium ferrocyanide (PFC) and protective effects of 2,4-dinitrophenol (DNP) under PFC treatment were tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with PFC at concentrations of 1.0 mM and mixtures with DNP in concentrations of 0.50 and 1.25 mM, eithe...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21134
更新日期：2013-11-01 00:00:00
abstract：:Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期：1996-01-01 00:00:00
abstract：:Plants of the Asteraceae and Hypericaceae possess secondary compounds that induce photooxidation in insect herbivores that consume them. One of the well-established modes of action of these substances is peroxidation of membrane lipids. Some herbivores counteract these defences by avoidance of light and tissues rich i...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940290210
更新日期：1995-01-01 00:00:00
abstract：:3,4-Dihydroxyphenylalanine, 5-6-dihydroxyindole, and N-acetylarterenone were detected by electrochemical methods in the hemolymph of immune reactive larvae of Drosophila melanogaster following parasitization by the wasp Leptopilina boulardi. Determinations of the catechols were made after separation by reverse phase, ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200303
更新日期：1992-01-01 00:00:00
abstract：:Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940150104
更新日期：1990-01-01 00:00:00
abstract：:Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid and two other C20 polyunsaturated fatty acids. Among other actions in invertebrates, PGs act in ovarian development, renal functions, immunity, hemocyte migration, and gene/protein expression. Reversible phosphorylation is a major mechanism of regulat...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21725
更新日期：2020-09-01 00:00:00
abstract：:Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.
更新日期：1999-01-01 00:00:00
abstract：:The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940210402
更新日期：1992-01-01 00:00:00
abstract：:Insect digestive proteinases are often strongly influenced by ambient physicochemical conditions, such as pH, ionic strength, and oxidation-reduction potential. Although the effects of the former two parameters are well documented, the influence of redox potential on catalytic rates of digestive enzymes is not well un...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/1520-6327(200008)44:4<151::AID-ARCH2>3.0.C
更新日期：2000-08-01 00:00:00
abstract：:Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20224
更新日期：2008-02-01 00:00:00
abstract：:A protocol has been developed for the purification of vitellogenin from the honey bee, Apis mellifera. Purification allows for the first characterization of a vitellogenin from the large order Hymenoptera. Hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotei...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940140405
更新日期：1990-01-01 00:00:00
abstract：:Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20118
更新日期：2006-05-01 00:00:00
abstract：:The gene encoding the Spodoptera frugiperda allatostatin type-A peptide family (Y/FXFGL-amides) was isolated from S. frugiperda brain cDNA. The gene encodes a precursor of 231 amino acids containing nine (or ten) Y/FXFGL-a peptides that are tandemly arranged in three blocks. The comparison of the Spofr-AST A precursor...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20003
更新日期：2004-07-01 00:00:00
abstract：:The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1998)38:4<185::AID-ARCH4>3
更新日期：1998-01-01 00:00:00
abstract：:A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was una...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21221
更新日期：2015-05-01 00:00:00
abstract：:Enzymes associated with glycogen metabolism and glycerol synthesis in larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, were investigated over the winter in 2000-2001. Glycerol content was scarcely detected in September and October, rapidly increased in November and December, peaked in January, ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10024
更新日期：2002-06-01 00:00:00
abstract：:Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules struct...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1997)34:3<313::AID-ARCH6>3
更新日期：1997-01-01 00:00:00
abstract：:cDNAs encoding two different epoxide hydrolases (nCfEH1 and nCfEH2) were cloned from a cDNA library prepared from the wandering larval stage of the cat flea, Ctenocephalides felis. Predicted translations of the open reading frames indicated the clones encoded proteins of 464 (CfEH1) and 465 (CfEH2) amino acids. These ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10044
更新日期：2002-08-01 00:00:00
abstract：:The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20255
更新日期：2008-06-01 00:00:00
abstract：:The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21073
更新日期：2013-02-01 00:00:00
