Abstract:
:A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These bands showed a proteolytic activity in zymography assay. Midgut enzymes showed optimum proteolytic activity at pH 9 and 35°C using N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenyl-alanine 4-nitroanilide as a substrate. The Michaelis constant (Km) and maximum reaction rate (Vmax) were 0.55±0.042 mM and 0.714±0.056 μmol p-nitroalanine produced min(-1) mg protein(-1) , respectively. Inhibition was affected by trypsin inhibitor, but not by phenylmethylsulfonyl fluoride and N-tosyl-L-phenylalanine chloromethyl ketone, which indicated the trypsin-like but not chymotrypsin-like specificity. The identity of the serine protease was confirmed by nanoliquid-tandem mass spectrometry. Eleven unique peptides of the B. terrestris serine protease were found. It shows high homology to a previously reported B. ignitus serine protease covering more than 65% of the protein amino acid sequence.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík Idoi
10.1002/arch.21075subject
Has Abstractpub_date
2013-03-01 00:00:00pages
117-28issue
3eissn
0739-4462issn
1520-6327journal_volume
82pub_type
杂志文章abstract::At 22°C and under a long-day photoperiod of L:D 16:8, all the last fifth instar Loxostege sticticalis larvae undergo prepupal stage and pupate without diapause. Under a short-day photoperiod of L:D 12:12, in contrast, they all enter diapause with approximately 36 days diapause maintenance and then terminate diapause s...
journal_title:Archives of insect biochemistry and physiology
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doi:10.1002/arch.21163
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journal_title:Archives of insect biochemistry and physiology
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abstract::The toxic manipulator of oogenesis (TomO) protein has been identified in the wMel strain of Wolbachia that symbioses with the vinegar fly Drosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21471
更新日期:2018-07-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章,评审
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.1063
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20367
更新日期:2010-06-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940140405
更新日期:1990-01-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10062
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pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3
更新日期:1998-01-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21306
更新日期:2016-01-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940300205
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更新日期:2009-01-01 00:00:00
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更新日期:2000-08-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20137
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abstract::The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...
journal_title:Archives of insect biochemistry and physiology
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