Abstract：
:Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception during late larval development. The molecular cloning of Met will be a step toward understanding this gene and possibly identifying a preadult role(s) for JH. Molecular cloning was initiated using the technique of transposon-tagging with a transposable P element. P-element insertional alleles of Met were generated, and genomic libraries were constructed from two of these alleles. From these libraries P-element-bearing clones were isolated that in situ hybridized to the cytogenetic region where Met had been previously localized by genetic methods. Two of the alleles were shown to have complete P-elements inserted in similar, but not identical, locations in the predicted cytogenetic region where Met is located. A late-larval cDNA library was screened to identify transcriptional units in this region, and clones were recovered with homology to a DNA fragment abutting the P-element insertion site. These clones may represent Met cDNA molecules.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Turner C,Wilson TGdoi
10.1002/arch.940300205subject
Has Abstractpub_date
1995-01-01 00:00:00pages
133-47issue
2-3eissn
0739-4462issn
1520-6327journal_volume
30pub_type
杂志文章abstract：:Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20079
更新日期：2005-09-01 00:00:00
abstract：:Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940170208
更新日期：1991-01-01 00:00:00
abstract：:Insect γ-aminobutyric acid receptors (GABARs) are important molecular targets of cyclodiene and phenylpyrazole insecticides. Previously GABARs encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. In this study, we cl...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21232
更新日期：2015-04-01 00:00:00
abstract：:cDNAs encoding two different epoxide hydrolases (nCfEH1 and nCfEH2) were cloned from a cDNA library prepared from the wandering larval stage of the cat flea, Ctenocephalides felis. Predicted translations of the open reading frames indicated the clones encoded proteins of 464 (CfEH1) and 465 (CfEH2) amino acids. These ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10044
更新日期：2002-08-01 00:00:00
abstract：:Acromelic acid, a naturally occurring kainoid, isolated from the mushroom Clitocybe acromelalga, is a weak displacer of [3H]L-glutamate binding to cockroach (Periplaneta americana) nerve cord membranes. Acromelic acid (1 mM) displaces approximately 60% of specifically bound [3H]L-glutamate. When applied by bath perfus...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940250202
更新日期：1994-01-01 00:00:00
abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200102
更新日期：1992-01-01 00:00:00
abstract：:A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21075
更新日期：2013-03-01 00:00:00
abstract：:THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20046
更新日期：2005-04-01 00:00:00
abstract：:The polyhydroxy structure of ecdysteroids provides a basis for the formation of various derivatives including the acetonides that have been frequently prepared mainly in preparative work. Acetonides can be formed between vicinal diols in cis position such as positions C-2/C-3 and C-20/C-22 in 20-hydroxyecdysone, for e...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1999)41:3<162::AID-ARCH8>3
更新日期：1999-01-01 00:00:00
abstract：:Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20193
更新日期：2007-07-01 00:00:00
abstract：:Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21243
更新日期：2015-09-01 00:00:00
abstract：:Neosurugatoxin (NSTX), a neurotoxin isolated from the Japanese ivory mollusc Babylonia japonica, is a potent neuronal nicotinic acetylcholine receptor (nAChR) antagonist. Hitherto, NSTX has been found to block only neuronal nAChRs that are insensitive to alpha-Bgt. Here, we report for the first time that NSTX blocks a...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940230403
更新日期：1993-01-01 00:00:00
abstract：:Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20069
更新日期：2005-12-01 00:00:00
abstract：:Calyx fluid and venom from the braconid parasitoid Microplitis demolitor differentially affected the development of Pseudoplusia includens and Heliothis virescens. P. includens exhibited delays in larval development, supernumerary instars, and formed larval-pupal intermediates when injected with 0.01-0.10 wasp equival...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940180302
更新日期：1991-01-01 00:00:00
abstract：:A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1997)35:1/2<45::AID-ARCH5>
更新日期：1997-01-01 00:00:00
abstract：:A major long-term goal of polydnavirus (PDV) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. As PDV viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20228
更新日期：2008-04-01 00:00:00
abstract：:Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the obser...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10119
更新日期：2004-01-01 00:00:00
abstract：:Nineteen 5-phenyloxazoles (5POs) were examined for their ability to modulate adenylate cyclase by measuring cAMP produced in head membrane homogenates of fifth instar larvae of the silkworm Bombyx mori. Among the compounds tested, 5-(4-methoxyphenyl)oxazole (9) and the 2,6-dichlorophenyl congener showed the highest ac...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10058
更新日期：2003-01-01 00:00:00
abstract：:The biological activity of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was examined in vivo toward larvae and pupae of Galleriae mellonella L. (Lepidoptera: Pyralidae), and in vitro toward bacterial and fungal cultures, as well as cultured insect cells. Pupae of G. mellonella were far more susceptibl...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20100
更新日期：2006-02-01 00:00:00
abstract：:Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940150104
更新日期：1990-01-01 00:00:00
abstract：:Molting in crustaceans is induced by ecdysteroids as in insects. The ecdysteroid titre in hemolymph is negatively regulated by molt-inhibiting hormone (MIH) that inhibits the secretion of ecdysteroids from the Y-organ, an ecdysteroid-producing gland of crustaceans, whereas little is known about the molecular mechanism...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20335
更新日期：2009-12-01 00:00:00
abstract：:Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20174
更新日期：2007-05-01 00:00:00
abstract：:When diapause and non-diapause eggs of the same bivoltine strain of Bombyx mori were chilled at 5 degrees C for more than 30 days, the hatchability of diapause eggs increased while that of non-diapause eggs decreased, respectively. To investigate the relationship between effects of chilling on the hatchability and the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20367
更新日期：2010-06-01 00:00:00
abstract：:There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20332
更新日期：2010-01-01 00:00:00
abstract：:Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21595
更新日期：2019-09-01 00:00:00
abstract：:Enzymes associated with glycogen metabolism and glycerol synthesis in larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, were investigated over the winter in 2000-2001. Glycerol content was scarcely detected in September and October, rapidly increased in November and December, peaked in January, ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10024
更新日期：2002-06-01 00:00:00
abstract：:Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20080
更新日期：2006-03-01 00:00:00
abstract：:In S. exigua, ingestion of Cry1Ac reduces larval growth, shortens lifespan, and decreases copulation and oviposition of the adults. Cadherin-like protein SeCad1b in S. exigua has recently been published. Here, we tested whether SeCad1b mediates the negative effects of Cry1Ac. We identified three potential Cry toxin bi...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21163
更新日期：2014-05-01 00:00:00
abstract：:Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreove...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20386
更新日期：2010-10-01 00:00:00
abstract：:Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940260207
更新日期：1994-01-01 00:00:00
