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Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura arctica (Onychiuridae: Collembola).

Abstract：

:The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H₂O₂), or dehydration, is well documented. In this respect, we measured the amount of H₂O₂ and antioxidant enzyme activities (superoxide dismutases: copper, zinc--CuZnSOD and manganese containing--MnSOD, and catalase--CAT), as the regulatory components determining H₂O₂ concentrations, in Arctic springtails incubated at 5 °C (control) versus -2 °C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in Drosophila, we measured the levels of the active physiological form of ecdysone--20-hydroxyecdysone (20-HE). Significantly elevated H₂O₂ and 20-HE levels were observed in M. arctica incubated at -2 °C, supporting a link between ecdysone, H₂O₂, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at -2 °C versus 5 °C, suggesting reduced H₂O₂ breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 °C (controls) versus -2 °C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at -2 °C, perhaps an additional source of H₂O₂. Our results suggest that H₂O₂ and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.

journal_name

Arch Insect Biochem Physiol

journal_title

Archives of insect biochemistry and physiology

authors

Grubor-Lajšić G,Petri ET,Kojić D,Purać J,Popović ZD,Worland RM,Clark MS,Mojović M,Blagojević DP

doi

10.1002/arch.21073

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

59-70

issue

eissn

0739-4462

issn

1520-6327

journal_volume

pub_type

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Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura arctica (Onychiuridae: Collembola).