Abstract：
:A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was unaltered over a wide range of temperature, pH, and in the presence of dithiothreitol. Larval midgut proteases were unable to digest PDTI for up to 12 h of incubation. Dixon and Lineweaver-Burk double reciprocal plots analysis revealed a competitive inhibition mechanism and a Ki of ∼3.9 × 10(-8) M. Lethal dose (0.50% w/w) and dosage for weight reduction by 50% (0.25% w/w) were determined. PDTI showed a dose-dependent effect on mean larval weight and a series of nutritional disturbances. In artificial diet at 0.25% w/w PDTI, the efficiency of conversion of ingested food, of digested food, relative growth rate, and growth index declined, whereas approximate digestibility, relative consumption rate, metabolic cost, consumption index, and total developmental period were increased in larvae. This is the first report of antifeedant and antimetabolic activities of PDTI on midgut proteases of H. armigera.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Pandey PK,Singh D,Jamal Fdoi
10.1002/arch.21221subject
Has Abstractpub_date
2015-05-01 00:00:00pages
18-34issue
1eissn
0739-4462issn
1520-6327journal_volume
89pub_type
杂志文章abstract：:This report concerns the effect of heat shock on host-pathogen interaction in Galleria mellonella infected with Bacillus thuringiensis. We show enhanced activity against Gram-positive bacteria in the hemolymph of larvae pre-exposed to heat shock before infection with B. thuringiensis. Heat shock influenced the protein...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21208
更新日期：2015-02-01 00:00:00
abstract：:Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10062
更新日期：2002-12-01 00:00:00
abstract：:Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20079
更新日期：2005-09-01 00:00:00
abstract：:Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期：1996-01-01 00:00:00
abstract：:Coexistence or displacement of parasitoids in hosts during intrinsic competitive interactions between different parasitoid species (multiparasitism) may depend on their life history traits and behavior. Intense competition for possession of hosts may lead to the elimination of the inferior competitor through physical ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21321
更新日期：2016-06-01 00:00:00
abstract：:A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1997)35:1/2<45::AID-ARCH5>
更新日期：1997-01-01 00:00:00
abstract：:When diapause and non-diapause eggs of the same bivoltine strain of Bombyx mori were chilled at 5 degrees C for more than 30 days, the hatchability of diapause eggs increased while that of non-diapause eggs decreased, respectively. To investigate the relationship between effects of chilling on the hatchability and the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20367
更新日期：2010-06-01 00:00:00
abstract：:The actions of acetylcholine and cholinergic ligands have been studied using dorsal midline neurones from the rnetathoracic ganglion of the cockroach Periplaneta americana.Both nicotine and oxotremorine depolarized dorsal midline neuronal cell bodies.Dose-response curves for nicotine and oxotremorine saturated at diff...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940210406
更新日期：1992-01-01 00:00:00
abstract：:The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10143
更新日期：2004-06-01 00:00:00
abstract：:Aphid, a short germband insect, displays an embryogenesis different from that of long germband insect species. Furthermore, the development of its parthenogenetic and viviparous embryo is different from that of the embryo resulting from sexual reproduction. To better understand the genetic regulation of this type of e...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21137
更新日期：2013-12-01 00:00:00
abstract：:Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章,评审
doi：10.1002/arch.940290204
更新日期：1995-01-01 00:00:00
abstract：:We exposed midgut cells from primary cultures of Heliothis virescens larvae to cell-free previously used medium, the Vaughn X and HyQ SFtrade mark media used for serum-free culture of insect cell lines which do not support H. virescens midgut cells, and to toxin from Bacillus thuringiensis. A statistically significant...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/1520-6327(200009)45:1<12::AID-ARCH2>3.0.CO
更新日期：2000-09-01 00:00:00
abstract：:Plants of the Asteraceae and Hypericaceae possess secondary compounds that induce photooxidation in insect herbivores that consume them. One of the well-established modes of action of these substances is peroxidation of membrane lipids. Some herbivores counteract these defences by avoidance of light and tissues rich i...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940290210
更新日期：1995-01-01 00:00:00
abstract：:We have used a parasitoid wasp Drosophila melanogaster system to investigate the relationship between the humoral and cellular immune responses in insects. Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was stu...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(200002)43:2<64::AID-ARCH2>
更新日期：2000-02-01 00:00:00
abstract：:The role of angiotensin converting enzyme (ACE, peptidyl dipeptidase A) in metamorphic- and reproductive-related events in the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae) was studied by using the selective ACE inhibitor captopril. Although oral administration of captopril had no effect on ...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20023
更新日期：2004-11-01 00:00:00
abstract：:The presence of allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata was confirmed by immunocytochemistry, bioassay, and HPLC. Immunocytochemical reactivity with monoclonal antibody against allatostatin I showed strong allatostatin immunoreactivity in the antennal hea...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200403
更新日期：1992-01-01 00:00:00
abstract：:Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20171
更新日期：2007-04-01 00:00:00
abstract：:The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as t...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1997)36:1<11::AID-ARCH2>3.
更新日期：1997-01-01 00:00:00
abstract：:The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20255
更新日期：2008-06-01 00:00:00
abstract：:Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20174
更新日期：2007-05-01 00:00:00
abstract：:The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G p...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20379
更新日期：2010-09-01 00:00:00
abstract：:THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20046
更新日期：2005-04-01 00:00:00
abstract：:This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1520-6327(1999)40:4<203::AID-ARCH5>3
更新日期：1999-01-01 00:00:00
abstract：:In vitro catabolism of juvenile hormone (JH) in haemolymph of adult female Cydia pomonella was ascribed mainly to juvenile hormone esterase (JHE) activity. No significant differences were noted between virgin and mated females 0-96 h post-emergence. Changes in JHE activity did not appear dependent upon fluctuations in...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.10007
更新日期：2002-01-01 00:00:00
abstract：:Calyx fluid and venom from the braconid parasitoid Microplitis demolitor differentially affected the development of Pseudoplusia includens and Heliothis virescens. P. includens exhibited delays in larval development, supernumerary instars, and formed larval-pupal intermediates when injected with 0.01-0.10 wasp equival...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940180302
更新日期：1991-01-01 00:00:00
abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.940200102
更新日期：1992-01-01 00:00:00
abstract：:Larvae of Sesamia nonagrioides developing under long day (LD) conditions pupate in the 5th or 6th instar, whereas under the short day (SD) conditions, they undergo several supernumerary larval molts and are regarded as diapausing. The development in early larval instars occurs in the LD larvae at a moderate and in the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20181
更新日期：2007-06-01 00:00:00
abstract：:Bostrichiformia is the less known major series of Coleoptera regarding digestive physiology. The midgut of Dermestes maculatus has a cylindrical ventriculus with anterior caeca. There is no cell differentiation along the ventriculus, except for the predominance of cells undergoing apocrine secretion in the anterior re...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20153
更新日期：2007-01-01 00:00:00
abstract：:Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.21332
更新日期：2016-07-01 00:00:00
abstract：:We cloned a full-length tyrosine hydroxylase cDNA from the integument of the diamondback moth, Plutella xylostella. In the phylogenetic tree, tyrosine hydroxylase (PxTH) clustered with the other lepidopteran THs. Serine residues in the PxTH sequence, namely Ser(24), Ser(31), Ser(35), Ser(53), and Ser(65), were predict...
journal_title：Archives of insect biochemistry and physiology
pub_type： 杂志文章
doi：10.1002/arch.20384
更新日期：2010-10-01 00:00:00
