Abstract:
:The actions of acetylcholine and cholinergic ligands have been studied using dorsal midline neurones from the rnetathoracic ganglion of the cockroach Periplaneta americana.Both nicotine and oxotremorine depolarized dorsal midline neuronal cell bodies.Dose-response curves for nicotine and oxotremorine saturated at different levels. Nicotine-induced depolarizations were completely or partially blocked by mecamylamine, d-tubocurarine, strychnine, and bicuculline, but were insensitive to alpha-bungarotoxin(100 nM), atropine (100 micronM),Scopolamine (10 micronM), and pirenzepine (50 micronM). Following pretreatment with collagenase, the dorsal midline neurones were sensitive to high doses of alpha-bungarotoxin (3 micronM). Oxotremorine-induced depolarizations were blocked by scopolamine (10 micronM) atropine (100 micronM), and pirenzepine (50 micronM) and were insensitive to mecamylamine (10 micronM) and d-tubocurarine (100 micronM). The results indicate the coexistence of at least two distinct acetylcholine receptors on dorsal midline neuronal cell bodies in the cockroach metathoracic ganglion.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Bai D,Erdbrugger H,Breer H,Sattelle DBdoi
10.1002/arch.940210406subject
Has Abstractpub_date
1992-01-01 00:00:00pages
289-301issue
4eissn
0739-4462issn
1520-6327journal_volume
21pub_type
杂志文章abstract::A protocol has been developed for the purification of vitellogenin from the honey bee, Apis mellifera. Purification allows for the first characterization of a vitellogenin from the large order Hymenoptera. Hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotei...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940140405
更新日期:1990-01-01 00:00:00
abstract::The gene encoding the Spodoptera frugiperda allatostatin type-A peptide family (Y/FXFGL-amides) was isolated from S. frugiperda brain cDNA. The gene encodes a precursor of 231 amino acids containing nine (or ten) Y/FXFGL-a peptides that are tandemly arranged in three blocks. The comparison of the Spofr-AST A precursor...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20003
更新日期:2004-07-01 00:00:00
abstract::Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10062
更新日期:2002-12-01 00:00:00
abstract::The role of angiotensin converting enzyme (ACE, peptidyl dipeptidase A) in metamorphic- and reproductive-related events in the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae) was studied by using the selective ACE inhibitor captopril. Although oral administration of captopril had no effect on ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20023
更新日期:2004-11-01 00:00:00
abstract::Aphid, a short germband insect, displays an embryogenesis different from that of long germband insect species. Furthermore, the development of its parthenogenetic and viviparous embryo is different from that of the embryo resulting from sexual reproduction. To better understand the genetic regulation of this type of e...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21137
更新日期:2013-12-01 00:00:00
abstract::Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20080
更新日期:2006-03-01 00:00:00
abstract::The Colorado potato beetle (Leptinotarsa decemlineata [Say]) is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lac...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21642
更新日期:2020-01-01 00:00:00
abstract::Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940150104
更新日期:1990-01-01 00:00:00
abstract::This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20412
更新日期:2011-04-01 00:00:00
abstract::The toxic manipulator of oogenesis (TomO) protein has been identified in the wMel strain of Wolbachia that symbioses with the vinegar fly Drosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21471
更新日期:2018-07-01 00:00:00
abstract::Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期:1996-01-01 00:00:00
abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20255
更新日期:2008-06-01 00:00:00
abstract::The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940210402
更新日期:1992-01-01 00:00:00
abstract::Pteromalus puparum is a gregarious pupal endoparasitoid with a wide host range. It deposits eggs into pierid and papilionid butterfly pupae. Glutathione S-transferases (GSTs) are a family of multifunctional detoxification enzymes that act in xenobiotic metabolism in insects. Insect genome projects have facilitated ide...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21634
更新日期:2020-02-01 00:00:00
abstract::Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21332
更新日期:2016-07-01 00:00:00
abstract::A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was una...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21221
更新日期:2015-05-01 00:00:00
abstract::A P-element mobility excision assay was used to determine if non-drosophilid insects could support P gene vector function. Present studies included the testing of Muscids, Sphaerocerids, and Phorids, none of which were able to support P mobility. A new excision indicator plasmid was developed allowing the detection an...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940220306
更新日期:1993-01-01 00:00:00
abstract::Lipid transfer particle (LTP) is present in hemolymph of the tobacco hornworm Manduca sexta. Biosynthesis of LTP, occurrence in hemolymph, and the role of LTP-apoproteins in the lipid transfer reaction were investigated using antibodies specific for LTP or for each of the apoproteins. In vitro protein synthesis follow...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)31:1<39::AID-ARCH3>3.
更新日期:1996-01-01 00:00:00
abstract::Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20069
更新日期:2005-12-01 00:00:00
abstract::A cDNA clone encoding a small GTP binding protein (Brho) was isolated from an embryonic cDNA library of Bombyx mori that encoded a polypeptide with 202 amino acids sharing 60-80% similarity with the Rho1 family of GTP binding proteins. The effector site and one of the guanine nucleotide binding sites differed from oth...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(200004)43:4<165::AID-ARCH2
更新日期:2000-04-01 00:00:00
abstract::Bostrichiformia is the less known major series of Coleoptera regarding digestive physiology. The midgut of Dermestes maculatus has a cylindrical ventriculus with anterior caeca. There is no cell differentiation along the ventriculus, except for the predominance of cells undergoing apocrine secretion in the anterior re...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20153
更新日期:2007-01-01 00:00:00
abstract::THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20046
更新日期:2005-04-01 00:00:00
abstract::The relatively little-investigated entomopathogen Conidiobolus coronatus secretes several proteinases into culture broth. Using a combination of ion-exchange and size-exclusion chromatography, we purified to homogeneity a serine proteinase of Mr 30,000-32,000, as ascertained by SDS-PAGE. The purified enzyme showed sub...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20134
更新日期:2006-08-01 00:00:00
abstract::This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1999)40:4<203::AID-ARCH5>3
更新日期:1999-01-01 00:00:00
abstract::The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viabl...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3
更新日期:1998-01-01 00:00:00
abstract::We cloned a full-length tyrosine hydroxylase cDNA from the integument of the diamondback moth, Plutella xylostella. In the phylogenetic tree, tyrosine hydroxylase (PxTH) clustered with the other lepidopteran THs. Serine residues in the PxTH sequence, namely Ser(24), Ser(31), Ser(35), Ser(53), and Ser(65), were predict...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20384
更新日期:2010-10-01 00:00:00
abstract::Chitin synthase catalyzes chitin synthesis in the exoskeleton, tracheal system and gut during insect development. A chitin synthase 1 (CfCHS1) cDNA was identified and cloned from the spruce budworm, Choristoneura fumiferana. The CfCHS1 cDNA is 5,300 bp in length and codes a 1,564-amino acid protein with a molecular ma...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20404
更新日期:2011-02-01 00:00:00
abstract::Molting in crustaceans is induced by ecdysteroids as in insects. The ecdysteroid titre in hemolymph is negatively regulated by molt-inhibiting hormone (MIH) that inhibits the secretion of ecdysteroids from the Y-organ, an ecdysteroid-producing gland of crustaceans, whereas little is known about the molecular mechanism...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20335
更新日期:2009-12-01 00:00:00
abstract::There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20332
更新日期:2010-01-01 00:00:00
abstract::In this study, larvae of silkworm Bombyx mori were subjected to low temperature, hypoxia, and viral infection to evaluate stressor-mediated oxidative stress (OS) and the induction of antioxidant enzymes (AOEs). Exposure to cold, hypoxia, and nuclear polyhedral virus for 24 h resulted in a significant increase in hydro...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21138
更新日期:2013-12-01 00:00:00