Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae).

abstract：:RNA interference (RNAi) has become an integral part of mainstream research due to its versatility and ease of use. However, the potential nontarget effects associated with double-stranded RNAs (dsRNA) are poorly understood. To explore this, we used dsRNAs targeting the inhibitor of apoptosis (iap) gene from nine insec...

journal_title：Archives of insect biochemistry and physiology

authors： Chereddy SCRR,Gurusamy D,Howell JL,Palli SR

更新日期：2020-08-01 00:00:00

abstract：:Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...

journal_title：Archives of insect biochemistry and physiology

authors： Puttaraju HP,Prakash BM

更新日期：2005-12-01 00:00:00

abstract：:A protocol has been developed for the purification of vitellogenin from the honey bee, Apis mellifera. Purification allows for the first characterization of a vitellogenin from the large order Hymenoptera. Hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotei...

journal_title：Archives of insect biochemistry and physiology

authors： Wheeler DE,Kawooya JK

更新日期：1990-01-01 00:00:00

abstract：:Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the...

journal_title：Archives of insect biochemistry and physiology

authors： Vignesvaran K,Alias Z

更新日期：2016-07-01 00:00:00

abstract：:Intracellular activities of K+, H+, Mg2+, Ca2+, and Cl-, measured with ion selective microelectrodes in the oocyte and the nurse cells in ovarian follicles of Hyalophora cecropia, indicated that a Ca2+ current is a key component of the electrical potential that is maintained across the intercellular bridges connecting...

journal_title：Archives of insect biochemistry and physiology

authors： Woodruff RI,Telfer WH

更新日期：1994-01-01 00:00:00

abstract：:An in vitro bioassay suitable for routine use to investigate hypertrehalosemic hormone (HTH)-dependent trehalose biosynthesis was developed for the cockroach fat body. Blaberus discoidalis fat bodies were isolated and divided so that eight matched pieces from a single tissue could be compared for multiple control and ...

journal_title：Archives of insect biochemistry and physiology

authors： Keeley LL,Sowa SM,Hesson AS

更新日期：1995-01-01 00:00:00

abstract：:Bt crop pyramids produce two or more Bt proteins active to broaden the spectrum of action and to delay the development of resistance in exposed insect populations. The cross-resistance between Bt toxins is a vital restriction factor for Bt crop pyramids, which may reduce the effect of pyramid strategy. In this review,...

journal_title：Archives of insect biochemistry and physiology

authors： Wei J,Zhang Y,An S

更新日期：2019-11-01 00:00:00

abstract：:Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...

journal_title：Archives of insect biochemistry and physiology

authors： Frantsevich L,Gorb S

更新日期：2006-05-01 00:00:00

abstract：:Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...

journal_title：Archives of insect biochemistry and physiology

authors： Berenbaum M

更新日期：1995-01-01 00:00:00

abstract：:Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...

journal_title：Archives of insect biochemistry and physiology

authors： Aurade RM,Jayalakshmi SK,Udikeri SS,Sreeramulu K

更新日期：2012-01-01 00:00:00

abstract：:Lipid transfer particle (LTP) is present in hemolymph of the tobacco hornworm Manduca sexta. Biosynthesis of LTP, occurrence in hemolymph, and the role of LTP-apoproteins in the lipid transfer reaction were investigated using antibodies specific for LTP or for each of the apoproteins. In vitro protein synthesis follow...

journal_title：Archives of insect biochemistry and physiology

authors： Van Heusden MC,Yepiz-Plascencia GM,Walker AM,Law JH

更新日期：1996-01-01 00:00:00

abstract：:Molting in crustaceans is induced by ecdysteroids as in insects. The ecdysteroid titre in hemolymph is negatively regulated by molt-inhibiting hormone (MIH) that inhibits the secretion of ecdysteroids from the Y-organ, an ecdysteroid-producing gland of crustaceans, whereas little is known about the molecular mechanism...

journal_title：Archives of insect biochemistry and physiology

authors： Asazuma H,Nagata S,Nagasawa H

更新日期：2009-12-01 00:00:00

abstract：:Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...

journal_title：Archives of insect biochemistry and physiology

authors： Martinez T,Wheeler D

更新日期：1991-01-01 00:00:00

abstract：:Two blue-pigment binding proteins, BP1 and BP2, are present in larval and pupal haemolymph of cabbage white butterfly, Pieris rapae, and fluctuate in expression during development. Both BP1 and BP2 are found in pupal haemolymph in varying proportions as well as in adult haemolymph, while only small amounts of BP2 are ...

journal_title：Archives of insect biochemistry and physiology

authors： Choi CW,Nam KP,Seo DH,Choi JW,Lee CS,Kim HR,Yun CY

更新日期：2006-04-01 00:00:00

abstract：:Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight bi...

journal_title：Archives of insect biochemistry and physiology

authors： Indrasith LS,Kindle H,Lanzrein B

更新日期：1990-01-01 00:00:00

abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...

journal_title：Archives of insect biochemistry and physiology

authors： Van Antwerpen R,Law JH

更新日期：1992-01-01 00:00:00

abstract：:Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic activity of its tran...

journal_title：Archives of insect biochemistry and physiology

authors： Shi M,Huang F,Chen YF,Meng XF,Chen XX

更新日期：2009-01-01 00:00:00

abstract：:Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward ...

journal_title：Archives of insect biochemistry and physiology

authors： Tsoumani KT,Meccariello A,Mathiopoulos KD,Papathanos PA

更新日期：2020-03-01 00:00:00

abstract：:We have employed the proteomic approach in combination with mass spectrometry to study the immune response of honey bee workers at different developmental stages. Analysis of the hemolymph proteins of noninfected, mock-infected and immune-challenged individuals by polyacrylamide gel electrophoresis showed differences ...

journal_title：Archives of insect biochemistry and physiology

authors： Randolt K,Gimple O,Geissendörfer J,Reinders J,Prusko C,Mueller MJ,Albert S,Tautz J,Beier H

更新日期：2008-12-01 00:00:00

abstract：:Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promo...

journal_title：Archives of insect biochemistry and physiology

authors： Tatsuke T,Lee JM,Kusakabe T,Iiyama K,Sezutsu H,Uchino K

更新日期：2013-04-01 00:00:00

abstract：:A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanoga...

journal_title：Archives of insect biochemistry and physiology

authors： Sonoda S,Tsumuki H

更新日期：2010-01-01 00:00:00

abstract：:Octopamine (OCT)/tyramine (TYR) analogues, mainly including p- and beta-substituted phenylethylamines, were prepared as probes for the ligand-binding site(s) of adenylate cyclase-coupled OCT or TYR receptors, and were examined for their effects on cAMP production in the head membranes of Bombyx mori larvae. Small stru...

journal_title：Archives of insect biochemistry and physiology

authors： Aoyama M,Nakane T,Ono T,Khan MA,Ohta H,Ozoe Y

更新日期：2001-05-01 00:00:00

abstract：:To determine differential gene expression profiles in the venom gland and sac (gland/sac) of a solitary hunting wasp species, Orancistrocerus drewseni Saussure (1857), a subtractive cDNA library was constructed by suppression subtractive hybridization. A total of 498 expressed sequence tags (EST) were clustered and as...

journal_title：Archives of insect biochemistry and physiology

authors： Baek JH,Woo TH,Kim CB,Park JH,Kim H,Lee S,Lee SH

更新日期：2009-08-01 00:00:00

abstract：:The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as t...

journal_title：Archives of insect biochemistry and physiology

authors： Girardie J,Chaabihi H,Fournier B,Lagueux M,Girardie A

更新日期：1997-01-01 00:00:00

abstract：:A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...

journal_title：Archives of insect biochemistry and physiology

authors： Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík I

更新日期：2013-03-01 00:00:00

abstract：:Series of agonists and antagonists were examined for their actions on a Bombyx morialpha-adrenergic-like octopamine receptor (OAR) stably expressed in HEK-293 cells. The rank order of potency of the agonists was clonidine>naphazoline>tolazoline in Ca(2+) mobilization assays, and that of the antagonists was chlorpromaz...

journal_title：Archives of insect biochemistry and physiology

authors： Huang J,Hamasaki T,Ozoe Y

更新日期：2010-02-01 00:00:00

abstract：:Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...

journal_title：Archives of insect biochemistry and physiology

authors： Schmelz EA,Grebenok RJ,Ohnmeiss TE,Bowers WS

更新日期：2002-12-01 00:00:00

abstract：:A Na(+)/H(+) exchanger (NHE) on the apical membrane of mosquito Malpighian tubule (MT) cells is believed to participate in the blood-feeding mosquito's vital secretion of Na(+) and fluid. This study presents the molecular cloning, primary structure, and tissue distribution of two cDNAs encoding Aedes aegypti mosquito ...

journal_title：Archives of insect biochemistry and physiology

authors： Hart SJ,Knezetic JA,Petzel DH

更新日期：2002-11-01 00:00:00

abstract：:Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception durin...

journal_title：Archives of insect biochemistry and physiology

authors： Turner C,Wilson TG

更新日期：1995-01-01 00:00:00

abstract：:In this study, we have analyzed the changes of the ovarian nutritional resources in Dipetalogaster maxima at representative days of the reproductive cycle: previtellogenesis, vitellogenesis, as well as fasting-induced early and late atresia. As expected, the amounts of ovarian lipids, proteins, and glycogen increased ...

journal_title：Archives of insect biochemistry and physiology

authors： Leyria J,Fruttero LL,Aguirre SA,Canavoso LE

更新日期：2014-11-01 00:00:00