Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.

Abstract:

:Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline.

authors

Tatsuke T,Lee JM,Kusakabe T,Iiyama K,Sezutsu H,Uchino K

doi

10.1002/arch.21083

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

173-82

issue

4

eissn

0739-4462

issn

1520-6327

journal_volume

82

pub_type

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