Insect cell stimulation by LPS requires the activity of cell-released proteases.

Abstract:

:The hemocyte line BTI-EA-1174-A from the lepidopteran insect Estigmene acraea responds to bacterial lipopolysaccharide (LPS) by an enhanced phagocytic reaction and a dose-dependent increase of lysozyme release [Wittwer et al., Dev Comp Immunol 21:323 (1997)]. This paper provides evidence for a strong proteolytic activity in cell culture supernatants occurring after addition of LPS (1 mg/ml). The proteolysis is caused by cell-released proteases and seems to be necessary for cell activation. Its inhibition by alpha 2-macroglobulin results in a dose-dependent reduction in cellular response strength. Phagocytic reactions, as well as lysozyme release, are lowered to about half in the presence of 0.0001 mg/ml alpha 2-macroglobulin. A nearly complete abolishment of activation was achieved with final concentrations of 1.0 mg/ml alpha 2-macroglobulin. The data presented allow us to conclude that the LPS-triggered proteolytic activity is an important part of the activation process; it occurs outside of the cells and delivers immune response activating factors.

authors

Wittwer D,Wiesner A

doi

10.1002/(SICI)1520-6327(1998)39:3<91::AID-ARCH1>3.

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

91-7

issue

3

eissn

0739-4462

issn

1520-6327

pii

10.1002/(SICI)1520-6327(1998)39:3<91::AID-ARCH1>3.

journal_volume

39

pub_type

杂志文章
  • Local expression and distribution of a storage protein in the ovary of Hyphantria cunea.

    abstract::Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study, the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and im...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.1063

    authors: Cheon HM,Kim HJ,Chung DH,Kim MO,Park JS,Yun CY,Seo SJ

    更新日期:2001-11-01 00:00:00

  • TWO INNEXINS OF Spodoptera litura INFLUENCES HEMICHANNEL AND GAP JUNCTION FUNCTIONS IN CELLULAR IMMUNE RESPONSES.

    abstract::Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21243

    authors: Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo K

    更新日期:2015-09-01 00:00:00

  • HPLC method for the analysis of acetonides of ecdysteroids providing structural information on different vicinal diols

    abstract::The polyhydroxy structure of ecdysteroids provides a basis for the formation of various derivatives including the acetonides that have been frequently prepared mainly in preparative work. Acetonides can be formed between vicinal diols in cis position such as positions C-2/C-3 and C-20/C-22 in 20-hydroxyecdysone, for e...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)41:3<162::AID-ARCH8>3

    authors: Kayser H,Eilinger P

    更新日期:1999-01-01 00:00:00

  • Free radical generation during the activation of hemolymph prepared from the homopteran Dactylopius coccus.

    abstract::Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20174

    authors: García-Gil De Muñoz F,Lanz-Mendoza H,Hernández-Hernández FC

    更新日期:2007-05-01 00:00:00

  • Plasmatocyte spreading peptide does not induce Microplitis demolitor polydnavirus-infected plasmatocytes to spread on foreign surfaces.

    abstract::Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.

    authors: Strand MR,Clark KC,Gardiner EM

    更新日期:1999-01-01 00:00:00

  • Starvation influences allatotropin gene expression and juvenile hormone titer in the female adult oriental armyworm, Mythimna separata.

    abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20255

    authors: Zhang L,Luo L,Jiang X

    更新日期:2008-06-01 00:00:00

  • Neosurugatoxin blocks an alpha-bungarotoxin-sensitive neuronal nicotinic acetylcholine receptor.

    abstract::Neosurugatoxin (NSTX), a neurotoxin isolated from the Japanese ivory mollusc Babylonia japonica, is a potent neuronal nicotinic acetylcholine receptor (nAChR) antagonist. Hitherto, NSTX has been found to block only neuronal nAChRs that are insensitive to alpha-Bgt. Here, we report for the first time that NSTX blocks a...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940230403

    authors: Bai D,Sattelle DB

    更新日期:1993-01-01 00:00:00

  • Unique features of flight muscles mitochondria of honey bees (Apis mellifera L.).

    abstract::Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21595

    authors: Syromyatnikov MY,Gureev AP,Vitkalova IY,Starkov AA,Popov VN

    更新日期:2019-09-01 00:00:00

  • Purification and characterization of arginine kinase from the American cockroach (Periplaneta americana).

    abstract::The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10143

    authors: Brown AE,France RM,Grossman SH

    更新日期:2004-06-01 00:00:00

  • Genes acting in longevity-related pathways in the endoparasitoid, Pteromalus puparum.

    abstract::Among insects, lifespans vary over a broad range, from the short-lived mayflies to the 17-year periodical cicadas. Generally, lifespans are determined by a phase in life, the reproductive lifespan, which varies among species. Numerous pathways, such as the insulin/insulin-like growth factor signaling pathway, the targ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21635

    authors: Xiong S,Yu K,Ye X,Fang Q,Deng Y,Xiao S,Yang L,Wang B,Wang F,Yan Z,Wang F,Song Q,Stanley DW,Ye G

    更新日期:2020-02-01 00:00:00

  • Courtship dances in the flies of the genus Lispe (Diptera: Muscidae): from the fly's viewpoint.

    abstract::Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20118

    authors: Frantsevich L,Gorb S

    更新日期:2006-05-01 00:00:00

  • Effects of ecdysteroid agonist RH-2485 reveal interactions between ecdysteroids and juvenile hormones in the development of Sesamia nonagrioides.

    abstract::Larvae of Sesamia nonagrioides developing under long day (LD) conditions pupate in the 5th or 6th instar, whereas under the short day (SD) conditions, they undergo several supernumerary larval molts and are regarded as diapausing. The development in early larval instars occurs in the LD larvae at a moderate and in the...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20181

    authors: Eizaguirre M,López C,Schafellner C,Sehnal F

    更新日期:2007-06-01 00:00:00

  • RNA interference-mediated knockdown of eye coloration genes in the western tarnished plant bug (Lygus hesperus Knight).

    abstract::Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21527

    authors: Brent CS,Hull JJ

    更新日期:2019-02-01 00:00:00

  • Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

    abstract::Apoptosis is a process of programmed cell death that is regulated by genes independently. The Bm30kc6 gene is a kind of small molecular lipoprotein about 30 kDa, expressed highly in the late stage of the silkworm hemolymph. Our study showed that overexpression of Bm30kc6 could decrease caspase-3 activation. Meanwhile,...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21741

    authors: Xiao Y,Li LL,Bibi A,Zhang N,Chen T,Mo Y,Yue W,Miao Y

    更新日期:2020-11-01 00:00:00

  • Juvenile hormone catabolism and oviposition in the codling moth, Cydia pomonella, as functions of age, mating status, and hormone treatment.

    abstract::In vitro catabolism of juvenile hormone (JH) in haemolymph of adult female Cydia pomonella was ascribed mainly to juvenile hormone esterase (JHE) activity. No significant differences were noted between virgin and mated females 0-96 h post-emergence. Changes in JHE activity did not appear dependent upon fluctuations in...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10007

    authors: Cole TJ,Ramaswamy SB,Srinivasan A,Dorn S

    更新日期:2002-01-01 00:00:00

  • AccERK2, a map kinase gene from Apis cerana cerana, plays roles in stress responses, developmental processes, and the nervous system.

    abstract::Extracellular signal-regulated kinase (ERK), a mitogen-activated protein kinase (MAPK), plays roles in a variety of cellular responses. However, limited information is available on the relationship between ERKs and environmental stresses. In this report, an ERK gene, AccERK2, was cloned and characterized from Apis cer...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21011

    authors: Li Y,Zhang L,Kang M,Guo X,Baohua Xu

    更新日期:2012-03-01 00:00:00

  • Applications of EcR gene switch technology in functional genomics.

    abstract::Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20193

    authors: Tavva VS,Palli SR,Dinkins RD,Collins GB

    更新日期:2007-07-01 00:00:00

  • Salivary glucose oxidase: multifunctional roles for helicoverpa zea?

    abstract::Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. We purified GOX 23-fold by isoelectric focusing of labial gland homogenates and investigated physical and kinetic properties of the enzyme. D-glucose and 6-deoxy-D-glucose were the optimal substrates of...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(199909)42:1<99::AID-ARCH10

    authors: Eichenseer H,Mathews MC,Bi JL,Murphy JB,Felton GW

    更新日期:1999-09-01 00:00:00

  • Effect of ecdysone agonists on vitellogenesis and the expression of EcR and USP in codling moth (Cydia pomonella).

    abstract::The effects of tebufenozide and methoxyfenozide on vitellogenin (Vg) synthesis/release in the fat body, translocation in hemolymph, uptake by the ovary, and the expression of the ecdysone receptor (EcR) and its heterodimer partner, ultraspiracle protein (USP) in fat body, were investigated in Cydia pomonella. The resu...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10073

    authors: Sun X,Song Q,Barrett B

    更新日期:2003-03-01 00:00:00

  • Pharmacological characterization of a Bombyx mori alpha-adrenergic-like octopamine receptor stably expressed in a mammalian cell line.

    abstract::Series of agonists and antagonists were examined for their actions on a Bombyx morialpha-adrenergic-like octopamine receptor (OAR) stably expressed in HEK-293 cells. The rank order of potency of the agonists was clonidine>naphazoline>tolazoline in Ca(2+) mobilization assays, and that of the antagonists was chlorpromaz...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20341

    authors: Huang J,Hamasaki T,Ozoe Y

    更新日期:2010-02-01 00:00:00

  • Use of the glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol as a probe to investigate hypertrehalosemic hormone-mediated change in carbohydrate and lipid metabolism in the cockroach, Periplaneta americana.

    abstract::The glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol (DIA) was used to investigate the action of hypertrehalosemic hormone (HTH) on carbohydrate, neutral lipid, and phospholipid metabolism in the hemolymph and fat body of the cockroach, Periplaneta americana. DIA blocked the hypertrehalosemic and hyperglycemic...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20152

    authors: Oguri E,Steele JE

    更新日期:2006-12-01 00:00:00

  • Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi-resistant cotton leafworm.

    abstract::Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, ha...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.1023

    authors: Smagghe G,Carton B,Decombel L,Tirry L

    更新日期:2001-03-01 00:00:00

  • Changes in the hemolymph protein profiles in Galleria mellonella infected with Bacillus thuringiensis involve apolipophorin III. The effect of heat shock.

    abstract::This report concerns the effect of heat shock on host-pathogen interaction in Galleria mellonella infected with Bacillus thuringiensis. We show enhanced activity against Gram-positive bacteria in the hemolymph of larvae pre-exposed to heat shock before infection with B. thuringiensis. Heat shock influenced the protein...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21208

    authors: Taszłow P,Wojda I

    更新日期:2015-02-01 00:00:00

  • In vivo and in vitro activity of venom from the endoparasitic wasp Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae).

    abstract::The biological activity of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) was examined in vivo toward larvae and pupae of Galleriae mellonella L. (Lepidoptera: Pyralidae), and in vitro toward bacterial and fungal cultures, as well as cultured insect cells. Pupae of G. mellonella were far more susceptibl...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20100

    authors: Ergin E,Uçkan F,Rivers DB,Sak O

    更新日期:2006-02-01 00:00:00

  • Differential expression of transcripts with potential relevance to chlorantraniliprole response in the Colorado potato beetle, Leptinotarsa decemlineata.

    abstract::The Colorado potato beetle (Leptinotarsa decemlineata [Say]) is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lac...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21642

    authors: Dumas P,Sambou M,Gaudet JD,Morin MD,Moffat CE,Boquel S,Jr Morin P

    更新日期:2020-01-01 00:00:00

  • Manduca sexta lipid transfer particle: synthesis by fat body and occurrence in hemolymph.

    abstract::Lipid transfer particle (LTP) is present in hemolymph of the tobacco hornworm Manduca sexta. Biosynthesis of LTP, occurrence in hemolymph, and the role of LTP-apoproteins in the lipid transfer reaction were investigated using antibodies specific for LTP or for each of the apoproteins. In vitro protein synthesis follow...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1996)31:1<39::AID-ARCH3>3.

    authors: Van Heusden MC,Yepiz-Plascencia GM,Walker AM,Law JH

    更新日期:1996-01-01 00:00:00

  • A functional analysis of the P-element gene-transfer vector in insects.

    abstract::A P-element mobility excision assay was used to determine if non-drosophilid insects could support P gene vector function. Present studies included the testing of Muscids, Sphaerocerids, and Phorids, none of which were able to support P mobility. A new excision indicator plasmid was developed allowing the detection an...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940220306

    authors: Handler AM,Gomez SP,O'Brochta DA

    更新日期:1993-01-01 00:00:00

  • Identification of vitellogenin in the ant, Camponotus festinatus: changes in hemolymph proteins and fat body development in workers.

    abstract::Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940170208

    authors: Martinez T,Wheeler D

    更新日期:1991-01-01 00:00:00

  • CLONING, EXPRESSION, AND FUNCTIONAL ANALYSIS OF THREE ODORANT-BINDING PROTEINS OF THE ORIENTAL FRUIT MOTH, Grapholita molesta (BUSCK) (LEPIDOPTERA: TORTRICIDAE).

    abstract::Odorant-binding proteins (OBPs) act in insect olfactory processes. OBPs are expressed in the olfactory organs and serve in binding and transport of hydrophobic odorants through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In this study, three OBP genes were cloned from the antennal t...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21309

    authors: Li GW,Zhang Y,Li YP,Wu JX,Xu XL

    更新日期:2016-02-01 00:00:00

  • Effect of the synergist, piperonyl butoxide, on the development of deltamethrin resistance in yellow fever mosquito, Aedes aegypti L. (Diptera: Culicidae).

    abstract::The larvae and adults of Aedes aegypti were tested for the potential to develop resistance to the synthetic pyrethroid, deltamethrin, alone or a combination of deltamethrin with the synergist, piperonyl butoxide (PBO). Although continuous larval selection for 40 generations resulted in 703-fold resistance, the resista...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10021

    authors: Kumar S,Thomas A,Sahgal A,Verma A,Samuel T,Pillai MK

    更新日期:2002-05-01 00:00:00