Abstract:
:The hemocyte line BTI-EA-1174-A from the lepidopteran insect Estigmene acraea responds to bacterial lipopolysaccharide (LPS) by an enhanced phagocytic reaction and a dose-dependent increase of lysozyme release [Wittwer et al., Dev Comp Immunol 21:323 (1997)]. This paper provides evidence for a strong proteolytic activity in cell culture supernatants occurring after addition of LPS (1 mg/ml). The proteolysis is caused by cell-released proteases and seems to be necessary for cell activation. Its inhibition by alpha 2-macroglobulin results in a dose-dependent reduction in cellular response strength. Phagocytic reactions, as well as lysozyme release, are lowered to about half in the presence of 0.0001 mg/ml alpha 2-macroglobulin. A nearly complete abolishment of activation was achieved with final concentrations of 1.0 mg/ml alpha 2-macroglobulin. The data presented allow us to conclude that the LPS-triggered proteolytic activity is an important part of the activation process; it occurs outside of the cells and delivers immune response activating factors.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Wittwer D,Wiesner Adoi
10.1002/(SICI)1520-6327(1998)39:3<91::AID-ARCH1>3.subject
Has Abstractpub_date
1998-01-01 00:00:00pages
91-7issue
3eissn
0739-4462issn
1520-6327pii
10.1002/(SICI)1520-6327(1998)39:3<91::AID-ARCH1>3.journal_volume
39pub_type
杂志文章abstract::Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study, the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and im...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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journal_title:Archives of insect biochemistry and physiology
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pub_type: 杂志文章
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journal_title:Archives of insect biochemistry and physiology
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journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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