Applications of EcR gene switch technology in functional genomics.

abstract：:Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...

journal_title：Archives of insect biochemistry and physiology

authors： Aurade RM,Jayalakshmi SK,Udikeri SS,Sreeramulu K

更新日期：2012-01-01 00:00:00

abstract：:Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study, the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and im...

journal_title：Archives of insect biochemistry and physiology

authors： Cheon HM,Kim HJ,Chung DH,Kim MO,Park JS,Yun CY,Seo SJ

更新日期：2001-11-01 00:00:00

abstract：:The role of angiotensin converting enzyme (ACE, peptidyl dipeptidase A) in metamorphic- and reproductive-related events in the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae) was studied by using the selective ACE inhibitor captopril. Although oral administration of captopril had no effect on ...

journal_title：Archives of insect biochemistry and physiology

authors： Vercruysse L,Gelman D,Raes E,Hooghe B,Vermeirssen V,Van Camp J,Smagghe G

更新日期：2004-11-01 00:00:00

abstract：:Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...

journal_title：Archives of insect biochemistry and physiology

authors： Strand MR,Clark KC,Gardiner EM

更新日期：1999-01-01 00:00:00

abstract：:The effects of tebufenozide and methoxyfenozide on vitellogenin (Vg) synthesis/release in the fat body, translocation in hemolymph, uptake by the ovary, and the expression of the ecdysone receptor (EcR) and its heterodimer partner, ultraspiracle protein (USP) in fat body, were investigated in Cydia pomonella. The resu...

journal_title：Archives of insect biochemistry and physiology

authors： Sun X,Song Q,Barrett B

更新日期：2003-03-01 00:00:00

abstract：:An active corpus allatum (CA) in the blood feeding insect, Rhodnius prolixus, releases a substance that inhibits metamorphosis in larvae, and activates egg production in adults. In adults, transecting the nervus corporis cardiacum II's (NCCII), which are attached posteriorly to each protocerebral lobe, greatly increas...

journal_title：Archives of insect biochemistry and physiology

authors： Chiang RG

更新日期：2000-05-01 00:00:00

abstract：:Pteromalus puparum is a gregarious pupal endoparasitoid with a wide host range. It deposits eggs into pierid and papilionid butterfly pupae. Glutathione S-transferases (GSTs) are a family of multifunctional detoxification enzymes that act in xenobiotic metabolism in insects. Insect genome projects have facilitated ide...

journal_title：Archives of insect biochemistry and physiology

authors： Xu G,Teng ZW,Gu GX,Guo L,Wang F,Xiao S,Wang JL,Wang BB,Fang Q,Wang F,Song QS,Stanley D,Ye GY

更新日期：2020-02-01 00:00:00

abstract：:The gene encoding the Spodoptera frugiperda allatostatin type-A peptide family (Y/FXFGL-amides) was isolated from S. frugiperda brain cDNA. The gene encodes a precursor of 231 amino acids containing nine (or ten) Y/FXFGL-a peptides that are tandemly arranged in three blocks. The comparison of the Spofr-AST A precursor...

journal_title：Archives of insect biochemistry and physiology

authors： Abdel-Latief M,Meyering-Vos M,Hoffmann KH

更新日期：2004-07-01 00:00:00

abstract：:The relatively little-investigated entomopathogen Conidiobolus coronatus secretes several proteinases into culture broth. Using a combination of ion-exchange and size-exclusion chromatography, we purified to homogeneity a serine proteinase of Mr 30,000-32,000, as ascertained by SDS-PAGE. The purified enzyme showed sub...

journal_title：Archives of insect biochemistry and physiology

authors： Bania J,Samborski J,Bogus M,Polanowski A

更新日期：2006-08-01 00:00:00

abstract：:This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...

journal_title：Archives of insect biochemistry and physiology

authors： Gazit Y,Dunkelblum E,Ben-Aziz O,Altstein M

更新日期：1992-01-01 00:00:00

abstract：:The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

journal_title：Archives of insect biochemistry and physiology

authors： Zhang L,Luo L,Jiang X

更新日期：2008-06-01 00:00:00

abstract：:Chitin synthase catalyzes chitin synthesis in the exoskeleton, tracheal system and gut during insect development. A chitin synthase 1 (CfCHS1) cDNA was identified and cloned from the spruce budworm, Choristoneura fumiferana. The CfCHS1 cDNA is 5,300 bp in length and codes a 1,564-amino acid protein with a molecular ma...

journal_title：Archives of insect biochemistry and physiology

authors： Ampasala DR,Zheng S,Zhang D,Ladd T,Doucet D,Krell PJ,Retnakaran A,Feng Q

更新日期：2011-02-01 00:00:00

abstract：:Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, ha...

journal_title：Archives of insect biochemistry and physiology

authors： Smagghe G,Carton B,Decombel L,Tirry L

更新日期：2001-03-01 00:00:00

abstract：:We have employed the proteomic approach in combination with mass spectrometry to study the immune response of honey bee workers at different developmental stages. Analysis of the hemolymph proteins of noninfected, mock-infected and immune-challenged individuals by polyacrylamide gel electrophoresis showed differences ...

journal_title：Archives of insect biochemistry and physiology

authors： Randolt K,Gimple O,Geissendörfer J,Reinders J,Prusko C,Mueller MJ,Albert S,Tautz J,Beier H

更新日期：2008-12-01 00:00:00

abstract：:A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...

journal_title：Archives of insect biochemistry and physiology

authors： Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík I

更新日期：2013-03-01 00:00:00

abstract：:There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for...

journal_title：Archives of insect biochemistry and physiology

authors： Lwalaba D,Hoffmann KH,Woodring J

更新日期：2010-01-01 00:00:00

abstract：:Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the propertie...

journal_title：Archives of insect biochemistry and physiology

authors： Syromyatnikov MY,Gureev AP,Vitkalova IY,Starkov AA,Popov VN

更新日期：2019-09-01 00:00:00

abstract：:V-ATPases are ubiquitous proton pumps found in eukaryotes, and are important in regulating the pH of cell compartments and in creating membrane potentials. The V-ATPase creates a proton gradient that is used as an energy source for the transport of other ions. The 16-kDa proteolipid is the proton-translocating subunit...

journal_title：Archives of insect biochemistry and physiology

authors： Holmes SP,Frazier SK,Pietrantonio PV

更新日期：2000-11-01 00:00:00

abstract：:Molting is the result of the expression of a cascade of genes that is sequentially both up and down-regulated by the molting hormone, 20-hydroxyecdysone (20E), which is secreted as a pulse during each instar. Benzoyl hydrazine analogs of 20E act like the native molting hormone at the molecular level by binding with th...

journal_title：Archives of insect biochemistry and physiology

authors： Retnakaran A,Krell P,Feng Q,Arif B

更新日期：2003-12-01 00:00:00

abstract：:The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viabl...

journal_title：Archives of insect biochemistry and physiology

authors： Komiya K,Agui N,Mitsuhashi J

更新日期：1998-01-01 00:00:00

abstract：:Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves...

journal_title：Archives of insect biochemistry and physiology

authors： Barbehenn R,Dodick T,Poopat U,Spencer B

更新日期：2005-09-01 00:00:00

abstract：:The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as t...

journal_title：Archives of insect biochemistry and physiology

authors： Girardie J,Chaabihi H,Fournier B,Lagueux M,Girardie A

更新日期：1997-01-01 00:00:00

abstract：:Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...

journal_title：Archives of insect biochemistry and physiology

authors： Berenbaum M

更新日期：1995-01-01 00:00:00

abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...

journal_title：Archives of insect biochemistry and physiology

authors： Sun Z,Yu J,Wu W,Zhang G

更新日期：2012-06-01 00:00:00

abstract：:The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G p...

journal_title：Archives of insect biochemistry and physiology

authors： Cheng Y,Luo L,Jiang X,Zhang L,Niu C

更新日期：2010-09-01 00:00:00

abstract：:In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...

journal_title：Archives of insect biochemistry and physiology

authors： Van Antwerpen R,Law JH

更新日期：1992-01-01 00:00:00

abstract：:Rab proteins play fundamental roles in the regulation of membrane traffic. Previously, from the brain of Bombyx mori we isolated two cDNA clones (BRab1 and BRab14), each of which encoded a different member of Rab-protein family and was expressed in Escherichia coli and purified using an affinity chromatography. In thi...

journal_title：Archives of insect biochemistry and physiology

authors： Uno T,Nakao A,Katsurauma C

更新日期：2004-10-01 00:00:00

abstract：:Destruxins have been implicated in the infection process by entomopathogenic fungi and have been also found to be highly toxic when applied topically or ingested by different insect species. To gain insight into the mechanism of action of this toxin on insect internal organs, we have evaluated the effects of destruxin...

journal_title：Archives of insect biochemistry and physiology

authors： Ruiz-Sanchez E,O'Donnell MJ

更新日期：2012-07-01 00:00:00

abstract：:Odorant-binding proteins (OBPs) act in insect olfactory processes. OBPs are expressed in the olfactory organs and serve in binding and transport of hydrophobic odorants through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In this study, three OBP genes were cloned from the antennal t...

journal_title：Archives of insect biochemistry and physiology

authors： Li GW,Zhang Y,Li YP,Wu JX,Xu XL

更新日期：2016-02-01 00:00:00

abstract：:Hydrocarbon and wax ester components of cuticular lipids of the braconid parasitoid Habrobracon hebetor Say reared at 25 degrees C on larvae of a pyralid moth have been identified by GC-MS and analyzed with respect to adult age, mating status, and diet. The hydrocarbons range in carbon number from C(21) to C(45) and c...

journal_title：Archives of insect biochemistry and physiology

authors： Howard RW,Baker JE

更新日期：2003-05-01 00:00:00