Abstract:
:The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viable for relatively long periods, and to continue to secrete ecdysteroids. Secretion of ecdysteroid by the PG in vitro became marked when the PG was taken from last instar larvae older than 2 days after the last molt. PGs cultured in any of the media secreted ecdysteroid only within the first 2 h after placing them in culture, however, in the MGM-450 medium, the PGs secreted ecdysteroid even after 5 days of culture.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Komiya K,Agui N,Mitsuhashi Jdoi
10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3subject
Has Abstractpub_date
1998-01-01 00:00:00pages
147-54issue
3eissn
0739-4462issn
1520-6327pii
10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3journal_volume
38pub_type
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