Abstract:
:Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still unclear. In this article, we reported a steady-state level of Inxs (SpliInxs) in hemocytes of Spodoptera litura, which formed nonfunctional hemichannels on the cell surface to maintain normal metabolism. We also reported that two innnexins (SpliInx2 and SpliInx3) were expressed significantly higher in hemocytes compared to other tissues, suggesting that they play important roles in hemocytes. Amino acid analysis found that two cysteine residues in two extracellular loops provided the capability for SpliInx2 and SpliInx3 hemichannels to dock into gap junctions. Western blotting demonstrated that both extracellular and intracellular loops of SpliInx3 and the extracellular loops of SpliInx2 might undergo posttranslational modification during the formation of a steady-state hemichannel. During hemichannel formation, SpliInx2 presented as one isoform, while SpliInx3 presented as three isoforms. These results provide fundamental knowledge for further study of how steady-state levels of SpliInxs are dynamically adjusted to perform cellular immune responses under immune challenge.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo Kdoi
10.1002/arch.21243subject
Has Abstractpub_date
2015-09-01 00:00:00pages
43-57issue
1eissn
0739-4462issn
1520-6327journal_volume
90pub_type
杂志文章abstract::Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期:1996-01-01 00:00:00
abstract::Immunoreactivity against peptides of the allatostatin family having a typical YXFGL-NH2 C-terminus has been localized in different areas of the central nervous system, stomatogastric nervous system and gut of the cockroach Blattella germanica. In the protocerebrum, the most characteristic immunoreactive perikarya are ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)37:4<269::AID-ARCH2>3
更新日期:1998-01-01 00:00:00
abstract::Insect digestive proteinases are often strongly influenced by ambient physicochemical conditions, such as pH, ionic strength, and oxidation-reduction potential. Although the effects of the former two parameters are well documented, the influence of redox potential on catalytic rates of digestive enzymes is not well un...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/1520-6327(200008)44:4<151::AID-ARCH2>3.0.C
更新日期:2000-08-01 00:00:00
abstract::The insect peptide hormone trypsin modulating oostatic factor (TMOF), a decapeptide that is synthesized by the mosquito ovary and controls the translation of the gut's trypsin mRNA was cloned and expressed in the marine alga Chlorella desiccata. To express Aedes aegypti TMOF gene (tmfA) in C. desiccata cells, two plas...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21306
更新日期:2016-01-01 00:00:00
abstract::Earlier work had shown that JH acts on the membrane of the follicle cell of Locusta migratoria, bringing about a rapid reduction in volume which can be detected in vitro by measuring the increase in optical path difference using quantitative interference microscopy. The juvenoid fenoxycarb, a phenoxyphenyl derivative,...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)32:3/4<613::AID-ARCH3
更新日期:1996-01-01 00:00:00
abstract::Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章,评审
doi:10.1002/arch.21652
更新日期:2020-03-01 00:00:00
abstract::Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20174
更新日期:2007-05-01 00:00:00
abstract::The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10143
更新日期:2004-06-01 00:00:00
abstract::Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940170208
更新日期:1991-01-01 00:00:00
abstract::Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21004
更新日期:2012-01-01 00:00:00
abstract::Adult functions of juvenile hormone (JH) have been described for Drosophila melanogaster and other dipteran insects, but preadult function for this hormone remains largely unknown in this order of insects. We have identified a mutation of Drosophila, Methoprene-tolerant (Met), which appears to alter JH reception durin...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940300205
更新日期:1995-01-01 00:00:00
abstract::The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21073
更新日期:2013-02-01 00:00:00
abstract::Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20064
更新日期:2005-07-01 00:00:00
abstract::A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was una...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21221
更新日期:2015-05-01 00:00:00
abstract::Juvenile hormone (JH) allows larval molting in response to ecdysteroids but prevents the switching of gene expression necessary for metamorphosis. I first review our efforts to isolate the nuclear receptor for JH in the larval epidermis of Manduca sexta using photoaffinity analogs and our recent findings that the mole...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1520-6327(1996)32:3/4<271::AID-ARCH2
更新日期:1996-01-01 00:00:00
abstract::Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.
更新日期:1999-01-01 00:00:00
abstract::Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20069
更新日期:2005-12-01 00:00:00
abstract::Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20224
更新日期:2008-02-01 00:00:00
abstract::Among insects, lifespans vary over a broad range, from the short-lived mayflies to the 17-year periodical cicadas. Generally, lifespans are determined by a phase in life, the reproductive lifespan, which varies among species. Numerous pathways, such as the insulin/insulin-like growth factor signaling pathway, the targ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21635
更新日期:2020-02-01 00:00:00
abstract::RNA interference (RNAi) has become an integral part of mainstream research due to its versatility and ease of use. However, the potential nontarget effects associated with double-stranded RNAs (dsRNA) are poorly understood. To explore this, we used dsRNAs targeting the inhibitor of apoptosis (iap) gene from nine insec...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21683
更新日期:2020-08-01 00:00:00
abstract::cDNAs encoding two different epoxide hydrolases (nCfEH1 and nCfEH2) were cloned from a cDNA library prepared from the wandering larval stage of the cat flea, Ctenocephalides felis. Predicted translations of the open reading frames indicated the clones encoded proteins of 464 (CfEH1) and 465 (CfEH2) amino acids. These ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10044
更新日期:2002-08-01 00:00:00
abstract::Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20080
更新日期:2006-03-01 00:00:00
abstract::Peroxides are formed in the midgut fluids of caterpillars when ingested tannins and other phenolic compounds oxidize. If these peroxides broke down in the presence of redox-active metal ions, they would form damaging free radicals (Fenton-type reactions). Elemental iron is present in relatively large amounts in leaves...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20079
更新日期:2005-09-01 00:00:00
abstract::The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative tra...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21739
更新日期:2020-12-01 00:00:00
abstract::A neuronal morphological phenotype can be induced in cultured Spodoptera frugiperda insect cells (Sf21) by supplementing serum-containing media with 20-hydroxyecdysone (20-HE) and/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21249
更新日期:2015-11-01 00:00:00
abstract::The presence of allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata was confirmed by immunocytochemistry, bioassay, and HPLC. Immunocytochemical reactivity with monoclonal antibody against allatostatin I showed strong allatostatin immunoreactivity in the antennal hea...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940200403
更新日期:1992-01-01 00:00:00
abstract::The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20376
更新日期:2010-11-01 00:00:00
abstract::An active corpus allatum (CA) in the blood feeding insect, Rhodnius prolixus, releases a substance that inhibits metamorphosis in larvae, and activates egg production in adults. In adults, transecting the nervus corporis cardiacum II's (NCCII), which are attached posteriorly to each protocerebral lobe, greatly increas...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(200005)44:1<1::AID-ARCH1>3
更新日期:2000-05-01 00:00:00
abstract::A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1997)35:1/2<45::AID-ARCH5>
更新日期:1997-01-01 00:00:00
abstract::Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20171
更新日期:2007-04-01 00:00:00