Allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata.

Abstract:

:The presence of allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata was confirmed by immunocytochemistry, bioassay, and HPLC. Immunocytochemical reactivity with monoclonal antibody against allatostatin I showed strong allatostatin immunoreactivity in the antennal heart nerve which innervates this muscle with varicosities along the muscle fibers and in the insertion of the muscle on the pulsatile ampullae. Bioassay of Sep-Pak purified muscle extract demonstrated inhibition of juvenile hormone synthesis by corpora allata in vitro. A dose-response curve showed maximum inhibition of juvenile hormone synthesis was achieved with 10-20 pulsatile organ muscle eq/corpora allata, and 50% inhibition achieved with an estimated 2.6 pulsatile organ muscle eq. Two successive HPLC separations of the Sep-Pak purified extract yielded bioactive fractions corresponding to the elution times of the five known allatostatins.

authors

Woodhead AP,Stoltzman CA,Stay B

doi

10.1002/arch.940200403

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

253-63

issue

4

eissn

0739-4462

issn

1520-6327

journal_volume

20

pub_type

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