Stimulation of midgut stem cell proliferation by Manduca sexta alpha-arylphorin.

Abstract:

:Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the observed stem cell proliferation. SDS-PAGE analysis of the protein results in a single band of 77 kDa. Sequences of tryptic peptides from this protein are identical to internal sequences of the storage hexamer alpha-arylphorin. The alpha-arylphorin isolated by our procedure represents a small fraction of the total arylphorin present in the fat body extract. However, it alone seems responsible for the stimulation of mitotic activity in H. virescens midgut stem cells.

authors

Blackburn MB,Loeb MJ,Clark E,Jaffe H

doi

10.1002/arch.10119

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

26-32

issue

1

eissn

0739-4462

issn

1520-6327

journal_volume

55

pub_type

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