Abstract:
:Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-induced trypsins are also inhibited by other PIs, which we posed as the hypothesis that Inga laurina trypsin inhibitor (ILTI) inhibits PI-induced trypsins in S. frugiperda. In the process of testing our hypothesis, we compared its properties with those of selected PIs, soybean Kunitz trypsin inhibitor (SKTI), Inga vera trypsin inhibitor (IVTI), Adenanthera pavonina trypsin inhibitor (ApTI), and Entada acaciifolia trypsin inhibitor (EATI). We report that ILTI is more effective in inhibiting the induced S. frugiperda trypsins than SKTI and the other PIs, which supports our hypothesis. ILTI may be more appropriate than SKTI for studies regarding adaptive mechanisms to dietary PIs.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Machado SW,de Oliveira CFR,Zério NG,Parra JRP,Macedo MLRdoi
10.1002/arch.21393subject
Has Abstractpub_date
2017-08-01 00:00:00issue
4eissn
0739-4462issn
1520-6327journal_volume
95pub_type
杂志文章abstract::A cell line derived from the embryos of the cotton boll weevil, Anthonomus grandis (BRL-AG-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20E). The cells respond to 10(-6) M 20E by inhibition of cell growth and enhanced production of some secreted proteins. Crude nuclear extracts ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1997)35:1/2<45::AID-ARCH5>
更新日期:1997-01-01 00:00:00
abstract::Lysibia nana is a solitary, secondary idiobiont hyperparasitoid that attacks newly cocooned pre-pupae and pupae of several closely related gregarious endoparasitoids in the genus Cotesia, including C. glomerata. Prior to oviposition, the female wasp injects paralysing venom into the host, thus preventing further devel...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20080
更新日期:2006-03-01 00:00:00
abstract::Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules struct...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1997)34:3<313::AID-ARCH6>3
更新日期:1997-01-01 00:00:00
abstract::Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20456
更新日期:2012-06-01 00:00:00
abstract::We have used a parasitoid wasp Drosophila melanogaster system to investigate the relationship between the humoral and cellular immune responses in insects. Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was stu...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(200002)43:2<64::AID-ARCH2>
更新日期:2000-02-01 00:00:00
abstract::The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G p...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20379
更新日期:2010-09-01 00:00:00
abstract::Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)33:2<83::AID-ARCH1>3.
更新日期:1996-01-01 00:00:00
abstract::A Na(+)/H(+) exchanger (NHE) on the apical membrane of mosquito Malpighian tubule (MT) cells is believed to participate in the blood-feeding mosquito's vital secretion of Na(+) and fluid. This study presents the molecular cloning, primary structure, and tissue distribution of two cDNAs encoding Aedes aegypti mosquito ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10057
更新日期:2002-11-01 00:00:00
abstract::This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20412
更新日期:2011-04-01 00:00:00
abstract::The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10143
更新日期:2004-06-01 00:00:00
abstract::The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effect...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20376
更新日期:2010-11-01 00:00:00
abstract::Many studies have documented the involvement of eicosanoids in insect cellular immune responses to bacteria. The use of the fungal pathogen Beauveria bassiana as a nodulation elicitor, with inhibition of phospholipase A(2) by dexamethasone, extends the principle to fungi. This study also provides the first evidence of...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10049
更新日期:2002-09-01 00:00:00
abstract::The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)38:4<185::AID-ARCH4>3
更新日期:1998-01-01 00:00:00
abstract::Salivary enzyme, glucose oxidase (GOX) from the caterpillar Helicoverpa zea, catalyzes the conversion of glucose to gluconic acid and hydrogen peroxide. Because hydrogen peroxide has well-known antimicrobial properties, we examined whether caterpillar labial saliva could reduce the infectivity of bacterial pathogens. ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20031
更新日期:2005-02-01 00:00:00
abstract::Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章,评审
doi:10.1002/arch.940290204
更新日期:1995-01-01 00:00:00
abstract::THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20046
更新日期:2005-04-01 00:00:00
abstract::In the hawkmoth Manduca sexta high density lipophorin from adult insects (HDLp-A) delivers lipids to developing oocytes. During this lipid delivery HDLp-A is taken up by the oocyte and converted to a very high density lipophorin (VHDLp), which is stored in protein storage granules (yolk bodies). A membrane-free lysate...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940200102
更新日期:1992-01-01 00:00:00
abstract::Earlier work had shown that JH acts on the membrane of the follicle cell of Locusta migratoria, bringing about a rapid reduction in volume which can be detected in vitro by measuring the increase in optical path difference using quantitative interference microscopy. The juvenoid fenoxycarb, a phenoxyphenyl derivative,...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1996)32:3/4<613::AID-ARCH3
更新日期:1996-01-01 00:00:00
abstract::Insect eye coloration arises from the accumulation of various pigments. A number of genes that function in the biosynthesis (vermilion, cinnabar, and cardinal) and importation (karmoisin, white, scarlet, and brown) of these pigments, and their precursors, have been identified in diverse species and used as markers for...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21527
更新日期:2019-02-01 00:00:00
abstract::Enzymes associated with glycogen metabolism and glycerol synthesis in larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis, were investigated over the winter in 2000-2001. Glycerol content was scarcely detected in September and October, rapidly increased in November and December, peaked in January, ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10024
更新日期:2002-06-01 00:00:00
abstract::The bioinsecticide Bacillus thuringiensis var. israelensis (Bti) is increasingly used worldwide for mosquito control. Although no established resistance to Bti has been described in the field so far, a resistant Aedes aegypti strain (LiTOX strain) was selected in the laboratory using field-collected leaf litter contai...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21076
更新日期:2013-02-01 00:00:00
abstract::Rab proteins play fundamental roles in the regulation of membrane traffic. Previously, from the brain of Bombyx mori we isolated two cDNA clones (BRab1 and BRab14), each of which encoded a different member of Rab-protein family and was expressed in Escherichia coli and purified using an affinity chromatography. In thi...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20014
更新日期:2004-10-01 00:00:00
abstract::Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940260207
更新日期:1994-01-01 00:00:00
abstract::Macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. Homologues of most of 36 originally discovered autophagy-related (ATG) genes in yeast have been characterized in higher eukaryotes inclu...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21212
更新日期:2015-01-01 00:00:00
abstract::A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanoga...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20339
更新日期:2010-01-01 00:00:00
abstract::Superoxide anion (O(-) (2)) and nitric oxide (NO) generation in Dactylopius coccus hemolymph obtained by perfusion and activated with zymosan was studied. Activated hemolymph reduced 3-[4,5 dimethylthiazolil-2]-2,5-diphenyl tetrazolium bromide. This reduction was prevented by superoxide dismutase (SOD) indicating O(-)...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20174
更新日期:2007-05-01 00:00:00
abstract::Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940170208
更新日期:1991-01-01 00:00:00
abstract::Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20193
更新日期:2007-07-01 00:00:00
abstract::Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20118
更新日期:2006-05-01 00:00:00
abstract::Extracellular signal-regulated kinase (ERK), a mitogen-activated protein kinase (MAPK), plays roles in a variety of cellular responses. However, limited information is available on the relationship between ERKs and environmental stresses. In this report, an ERK gene, AccERK2, was cloned and characterized from Apis cer...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21011
更新日期:2012-03-01 00:00:00