Depletion of autophagy-related genes ATG3 and ATG5 in Tenebrio molitor leads to decreased survivability against an intracellular pathogen, Listeria monocytogenes.

Abstract:

:Macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. Homologues of most of 36 originally discovered autophagy-related (ATG) genes in yeast have been characterized in higher eukaryotes including insects. In this study, the homologues of ATG3 (TmATG3) and ATG5 (TmATG5) were isolated from the coleopteran beetle, Tenebrio molitor by expressed sequence tag and RNAseq approaches. The cDNA of TmATG3 and TmATG5 comprise open-reading frame sizes of 963 and 792 bp encoding polypeptides of 320 and 263 amino acid residues, respectively. TmATG3 and TmATG5 mRNA are expressed in all developmental stages, and mainly in fat body and hemocytes of larvae. TmATG3 and TmATG5 showed an overall sequence identity of 58-95% to other insect Atg proteins. There exist clear one-to-one orthologs of TmATG3 and TmATG5 in Tribolium and that they clustered together in the gene tree. Depletion of TmATG3 and TmATG5 by RNA interference led to a significant reduction in survival ability of T. molitor larvae against an intracellular pathogen, Listeria monocytogenes. Six days post-Listeria challenge, the survival rate in the dsEGFP-injected (where EGFP is enhanced green fluorescent protein) control larvae was significantly higher (55%) compared to 4 and 3% for TmATG3 and TmATG5 double-stranded RNA injected larvae, respectively. These data suggested that TmATG3 and TmATG5 may play putative role in mediating autophagy-based clearance of Listeria in T. molitor model.

authors

Tindwa H,Jo YH,Patnaik BB,Noh MY,Kim DH,Kim I,Han YS,Lee YS,Lee BL,Kim NJ

doi

10.1002/arch.21212

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

85-99

issue

1

eissn

0739-4462

issn

1520-6327

journal_volume

88

pub_type

杂志文章
  • Neural inhibition of the corpus allatum in the last larval instar differs from that in adults in the blood-feeding insect, Rhodnius prolixus.

    abstract::An active corpus allatum (CA) in the blood feeding insect, Rhodnius prolixus, releases a substance that inhibits metamorphosis in larvae, and activates egg production in adults. In adults, transecting the nervus corporis cardiacum II's (NCCII), which are attached posteriorly to each protocerebral lobe, greatly increas...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(200005)44:1<1::AID-ARCH1>3

    authors: Chiang RG

    更新日期:2000-05-01 00:00:00

  • TWO INNEXINS OF Spodoptera litura INFLUENCES HEMICHANNEL AND GAP JUNCTION FUNCTIONS IN CELLULAR IMMUNE RESPONSES.

    abstract::Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21243

    authors: Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo K

    更新日期:2015-09-01 00:00:00

  • Biochemical mode of action and differential activity of new ecdysone agonists against mosquitoes and moths.

    abstract::THQ (1-aroyl-4-(arylamino)-1,2,3,4-tetrahydroquinoline) compounds were identified by FMC Corporation in cell-based assays that used ecdysone receptors from Drosophila melanogaster, Heliothis virescens, or Plodia interpunctata. THQ compounds showed weak insecticidal activity against H. virescens and, therefore, were no...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20046

    authors: Palli SR,Tice CM,Margam VM,Clark AM

    更新日期:2005-04-01 00:00:00

  • Starvation influences allatotropin gene expression and juvenile hormone titer in the female adult oriental armyworm, Mythimna separata.

    abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20255

    authors: Zhang L,Luo L,Jiang X

    更新日期:2008-06-01 00:00:00

  • Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae).

    abstract::This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20412

    authors: Mesquita-Rodrigues C,Saboia-Vahia L,Cuervo P,Levy CM,Honorio NA,Domont GB,de Jesus JB

    更新日期:2011-04-01 00:00:00

  • LARVAL X-RAY IRRADIATION INFLUENCES PROTEIN EXPRESSION IN PUPAE OF THE ORIENTAL FRUIT FLY, BACTROCERA DORSALIS.

    abstract::The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21330

    authors: Chang CL,Goodman CL,Ringbauer J,Geib SM,Stanley D

    更新日期:2016-07-01 00:00:00

  • Apoptosis in cultured midgut cells from heliothis virescens larvae exposed to various conditions.

    abstract::We exposed midgut cells from primary cultures of Heliothis virescens larvae to cell-free previously used medium, the Vaughn X and HyQ SFtrade mark media used for serum-free culture of insect cell lines which do not support H. virescens midgut cells, and to toxin from Bacillus thuringiensis. A statistically significant...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/1520-6327(200009)45:1<12::AID-ARCH2>3.0.CO

    authors: Loeb MJ,Hakim RS,Martin P,Narang N,Goto S,Takeda M

    更新日期:2000-09-01 00:00:00

  • Effect of the synergist, piperonyl butoxide, on the development of deltamethrin resistance in yellow fever mosquito, Aedes aegypti L. (Diptera: Culicidae).

    abstract::The larvae and adults of Aedes aegypti were tested for the potential to develop resistance to the synthetic pyrethroid, deltamethrin, alone or a combination of deltamethrin with the synergist, piperonyl butoxide (PBO). Although continuous larval selection for 40 generations resulted in 703-fold resistance, the resista...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10021

    authors: Kumar S,Thomas A,Sahgal A,Verma A,Samuel T,Pillai MK

    更新日期:2002-05-01 00:00:00

  • Phototoxicity of plant secondary metabolites: insect and mammalian perspectives.

    abstract::Phototoxic chemicals produced by plants have been studied in a number of contexts, most notably as protective agents against mammalian and insect herbivores. Although there are commonalities in the responses of these two groups of herbivores to plant phototoxins, there are differences as well. Whereas a greater range ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章,评审

    doi:10.1002/arch.940290204

    authors: Berenbaum M

    更新日期:1995-01-01 00:00:00

  • Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.

    abstract::The southern green stink bug (SGSB, Nezara viridula) is an emerging polyphagous pest in many regions of the world. RNA interference (RNAi) is a valuable method for understanding gene function and holds great potential for pest management. However, RNAi efficiency is variable among insects and the differences in transp...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21692

    authors: Gurusamy D,Howell JL,Chereddy SCRR,Koo J,Palli SR

    更新日期:2020-08-01 00:00:00

  • Sex pheromone biosynthesis in the processionary moth Thaumetopoea pityocampa by delta-13 desaturation.

    abstract::In vivo treatments of female sex pheromone glands of the processionary moth, Thaumetopoea pityocampa, with mass-labeled fatty acids showed that (Z)-13-hexadecen-11-ynyl acetate, the main sex pheromone component, is biosynthesized from palmitic acid by the combined action of delta-11 and delta-13 desaturases. The invol...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940140106

    authors: Arsequell G,Fabriàs G,Camps F

    更新日期:1990-01-01 00:00:00

  • Inhibitory effect of molt-inhibiting hormone on phantom expression in the Y-organ of the kuruma prawn, Marsupenaeus japonicus.

    abstract::Molting in crustaceans is induced by ecdysteroids as in insects. The ecdysteroid titre in hemolymph is negatively regulated by molt-inhibiting hormone (MIH) that inhibits the secretion of ecdysteroids from the Y-organ, an ecdysteroid-producing gland of crustaceans, whereas little is known about the molecular mechanism...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20335

    authors: Asazuma H,Nagata S,Nagasawa H

    更新日期:2009-12-01 00:00:00

  • Effects of ecdysteroid agonist RH-2485 reveal interactions between ecdysteroids and juvenile hormones in the development of Sesamia nonagrioides.

    abstract::Larvae of Sesamia nonagrioides developing under long day (LD) conditions pupate in the 5th or 6th instar, whereas under the short day (SD) conditions, they undergo several supernumerary larval molts and are regarded as diapausing. The development in early larval instars occurs in the LD larvae at a moderate and in the...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20181

    authors: Eizaguirre M,López C,Schafellner C,Sehnal F

    更新日期:2007-06-01 00:00:00

  • A novel type of hemocytes localizing melanization with high-spreading behavior in Mythimna separata.

    abstract::Lepidopteran larvae show a cellular response to invading foreign substances that are larger than hemocytes, for example, parasitoid eggs or larvae. This response is called hemocyte encapsulation and is often accompanied by phenoloxidase (PO)-catalyzed melanization. In the present study, we artificially transplanted en...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21173

    authors: Kato Y,Yoshida T,Miura K,Tanaka T,Nakamatsu Y,Ochiai M

    更新日期:2014-08-01 00:00:00

  • Effects of the juvenile hormone analog pyriproxyfen on German cockroach, Blattella germanica (L.), tergal gland development and production of tergal gland secretion proteins.

    abstract::Male German cockroaches possess secretory glands that secrete fluid into a pair of transverse depressions on the seventh and eighth abdominal tergites. We investigated the effects of altered juvenoid titer during the first part of the last instar on tergal gland secretory tissue development and the production of terga...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20137

    authors: Saltzmann KA,Saltzmann KD,Neal JJ,Scharf ME,Bennett GW

    更新日期:2006-09-01 00:00:00

  • Modulation of P-glycoprotein ATPase of Helicoverpa armigera by cholesterol: effects on ATPase activity and interaction of insecticides.

    abstract::Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to b...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21004

    authors: Aurade RM,Jayalakshmi SK,Udikeri SS,Sreeramulu K

    更新日期:2012-01-01 00:00:00

  • Identification of yolk platelet-associated hydrolases in the oocytes of Rhodnius prolixus.

    abstract::The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940210402

    authors: Nussenzveig RH,Oliveira PL,Masuda H

    更新日期:1992-01-01 00:00:00

  • Juvenile hormone changes associated with diapause induction, maintenance, and termination in the beet webworm, Loxostege sticticalis (Lepidoptera: Pyralidae).

    abstract::At 22°C and under a long-day photoperiod of L:D 16:8, all the last fifth instar Loxostege sticticalis larvae undergo prepupal stage and pupate without diapause. Under a short-day photoperiod of L:D 12:12, in contrast, they all enter diapause with approximately 36 days diapause maintenance and then terminate diapause s...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20429

    authors: Jiang X,Huang S,Luo L

    更新日期:2011-07-01 00:00:00

  • Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

    abstract::Apoptosis is a process of programmed cell death that is regulated by genes independently. The Bm30kc6 gene is a kind of small molecular lipoprotein about 30 kDa, expressed highly in the late stage of the silkworm hemolymph. Our study showed that overexpression of Bm30kc6 could decrease caspase-3 activation. Meanwhile,...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21741

    authors: Xiao Y,Li LL,Bibi A,Zhang N,Chen T,Mo Y,Yue W,Miao Y

    更新日期:2020-11-01 00:00:00

  • Cloning of the 16-kDa V-ATPase proteolipid subunit from the red imported fire ant Solenopsis invicta buren (Hymenoptera: Formicidae).

    abstract::V-ATPases are ubiquitous proton pumps found in eukaryotes, and are important in regulating the pH of cell compartments and in creating membrane potentials. The V-ATPase creates a proton gradient that is used as an energy source for the transport of other ions. The 16-kDa proteolipid is the proton-translocating subunit...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/1520-6327(200011)45:3<109::AID-ARCH2>3.0.C

    authors: Holmes SP,Frazier SK,Pietrantonio PV

    更新日期:2000-11-01 00:00:00

  • CLONING, EXPRESSION, AND FUNCTIONAL ANALYSIS OF THREE ODORANT-BINDING PROTEINS OF THE ORIENTAL FRUIT MOTH, Grapholita molesta (BUSCK) (LEPIDOPTERA: TORTRICIDAE).

    abstract::Odorant-binding proteins (OBPs) act in insect olfactory processes. OBPs are expressed in the olfactory organs and serve in binding and transport of hydrophobic odorants through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In this study, three OBP genes were cloned from the antennal t...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21309

    authors: Li GW,Zhang Y,Li YP,Wu JX,Xu XL

    更新日期:2016-02-01 00:00:00

  • Effect of ecdysone agonists on vitellogenesis and the expression of EcR and USP in codling moth (Cydia pomonella).

    abstract::The effects of tebufenozide and methoxyfenozide on vitellogenin (Vg) synthesis/release in the fat body, translocation in hemolymph, uptake by the ovary, and the expression of the ecdysone receptor (EcR) and its heterodimer partner, ultraspiracle protein (USP) in fat body, were investigated in Cydia pomonella. The resu...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10073

    authors: Sun X,Song Q,Barrett B

    更新日期:2003-03-01 00:00:00

  • Comparative analysis of two biliproteins, BP1 and BP2, from haemolymph of cabbage white butterfly, Pieris rapae.

    abstract::Two blue-pigment binding proteins, BP1 and BP2, are present in larval and pupal haemolymph of cabbage white butterfly, Pieris rapae, and fluctuate in expression during development. Both BP1 and BP2 are found in pupal haemolymph in varying proportions as well as in adult haemolymph, while only small amounts of BP2 are ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20106

    authors: Choi CW,Nam KP,Seo DH,Choi JW,Lee CS,Kim HR,Yun CY

    更新日期:2006-04-01 00:00:00

  • Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera.

    abstract::This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940190404

    authors: Gazit Y,Dunkelblum E,Ben-Aziz O,Altstein M

    更新日期:1992-01-01 00:00:00

  • Use of the glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol as a probe to investigate hypertrehalosemic hormone-mediated change in carbohydrate and lipid metabolism in the cockroach, Periplaneta americana.

    abstract::The glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol (DIA) was used to investigate the action of hypertrehalosemic hormone (HTH) on carbohydrate, neutral lipid, and phospholipid metabolism in the hemolymph and fat body of the cockroach, Periplaneta americana. DIA blocked the hypertrehalosemic and hyperglycemic...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20152

    authors: Oguri E,Steele JE

    更新日期:2006-12-01 00:00:00

  • Effect of culture medium on the in vitro secretion activity of prothoracic glands from Pseudaletia separata.

    abstract::The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viabl...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3

    authors: Komiya K,Agui N,Mitsuhashi J

    更新日期:1998-01-01 00:00:00

  • Juvenile hormone: the status of its "status quo" action.

    abstract::Juvenile hormone (JH) allows larval molting in response to ecdysteroids but prevents the switching of gene expression necessary for metamorphosis. I first review our efforts to isolate the nuclear receptor for JH in the larval epidermis of Manduca sexta using photoaffinity analogs and our recent findings that the mole...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1520-6327(1996)32:3/4<271::AID-ARCH2

    authors: Riddiford LM

    更新日期:1996-01-01 00:00:00

  • Stimulation of midgut stem cell proliferation by Manduca sexta alpha-arylphorin.

    abstract::Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the obser...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10119

    authors: Blackburn MB,Loeb MJ,Clark E,Jaffe H

    更新日期:2004-01-01 00:00:00

  • RNA interference-mediated knockdown and virus-induced suppression of Troponin C gene adversely affect the behavior or fitness of the green rice leafhopper, Nephotettix cincticeps.

    abstract::The green rice leafhopper, Nephotettix cincticeps, is a major rice pest in Southeast Asia and Southern China. Novel control strategies must be explored to control the rice pest. Behavior or fitness regulation of insect by modulating the Troponin C (TnC) may be a novel strategy in the comprehensive management of the in...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21438

    authors: Lan H,Hong X,Huang R,Lin X,Li Q,Li K,Zhou T

    更新日期:2018-02-01 00:00:00

  • Plasmatocyte spreading peptide does not induce Microplitis demolitor polydnavirus-infected plasmatocytes to spread on foreign surfaces.

    abstract::Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.

    authors: Strand MR,Clark KC,Gardiner EM

    更新日期:1999-01-01 00:00:00