Abstract:
:Bostrichiformia is the less known major series of Coleoptera regarding digestive physiology. The midgut of Dermestes maculatus has a cylindrical ventriculus with anterior caeca. There is no cell differentiation along the ventriculus, except for the predominance of cells undergoing apocrine secretion in the anterior region. Apocrine secretion affects a larger extension and a greater number of cells in caeca than in ventriculus. Ventricular cells putatively secrete digestive enzymes, whereas caecal cells are supposed to secrete peritrophic gel (PG) glycoproteins. Feeding larvae with dyes showed that caeca are water-absorbing, whereas the posterior ventriculus is water-secreting. Midgut dissection revealed a PG and a peritrophic membrane (PM) covering the contents in anterior and posterior ventriculus, respectively. This was confirmed by in situ chitin detection with FITC-WGA conjugates. Ion-exchange chromatography of midgut homogenates, associated with enzymatic assays with natural and synthetic substrates and specific inhibitors, showed that trypsin and chymotrypsin are the major proteinases, cysteine proteinase is absent, and aspartic proteinase probably is negligible. Amylase and trypsin occur in contents and decrease along the ventriculus; the contrary is true for cell-membrane-bound aminopeptidase. Maltase is cell-membrane-bound and predominates in anterior and middle midgut. Digestive enzyme activities in hindgut are negligible. This, together with dye data, indicates that enzymes are recovered from inside PM by a posterior-anterior flux of fluid outside PM before being excreted. The combined results suggest that protein digestion starts in anterior midgut and ends in the surface of posterior midgut cells. All glycogen digestion takes place in anterior midgut.
journal_name
Arch Insect Biochem Physioljournal_title
Archives of insect biochemistry and physiologyauthors
Caldeira W,Dias AB,Terra WR,Ribeiro AFdoi
10.1002/arch.20153subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1-18issue
1eissn
0739-4462issn
1520-6327journal_volume
64pub_type
杂志文章abstract::Nineteen 5-phenyloxazoles (5POs) were examined for their ability to modulate adenylate cyclase by measuring cAMP produced in head membrane homogenates of fifth instar larvae of the silkworm Bombyx mori. Among the compounds tested, 5-(4-methoxyphenyl)oxazole (9) and the 2,6-dichlorophenyl congener showed the highest ac...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10058
更新日期:2003-01-01 00:00:00
abstract::In vivo treatments of female sex pheromone glands of the processionary moth, Thaumetopoea pityocampa, with mass-labeled fatty acids showed that (Z)-13-hexadecen-11-ynyl acetate, the main sex pheromone component, is biosynthesized from palmitic acid by the combined action of delta-11 and delta-13 desaturases. The invol...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940140106
更新日期:1990-01-01 00:00:00
abstract::Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study, the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and im...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.1063
更新日期:2001-11-01 00:00:00
abstract::A major long-term goal of polydnavirus (PDV) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. As PDV viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20228
更新日期:2008-04-01 00:00:00
abstract::The southern green stink bug (SGSB, Nezara viridula) is an emerging polyphagous pest in many regions of the world. RNA interference (RNAi) is a valuable method for understanding gene function and holds great potential for pest management. However, RNAi efficiency is variable among insects and the differences in transp...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21692
更新日期:2020-08-01 00:00:00
abstract::Bursicon is a neuropeptide that regulates cuticle sclerotization (hardening and tanning) via a G protein-coupled receptor. It consists of two subunits, an alpha and a beta. In the present study, we investigated the transcriptional expression and in situ localization of bursicon alpha and beta in the central nerve syst...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20239
更新日期:2008-06-01 00:00:00
abstract::Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20224
更新日期:2008-02-01 00:00:00
abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20255
更新日期:2008-06-01 00:00:00
abstract::Macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. Homologues of most of 36 originally discovered autophagy-related (ATG) genes in yeast have been characterized in higher eukaryotes inclu...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21212
更新日期:2015-01-01 00:00:00
abstract::The hemolymph of each noctuid species successfully parasitized by Chelonus near curvimaculatus possessed a parasitism-specific protein (PSP) previously identified in host T. ni (Insect Biochem. 19:445;21:845). Expression of PSP occurred in a stage-specific manner in the stadium during which the host undergoes precocio...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940240305
更新日期:1993-01-01 00:00:00
abstract::The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21330
更新日期:2016-07-01 00:00:00
abstract::Vitellogenin has been identified in the ant Camponotus festinatus, both in queens and workers. In the workers, it is already present before adult eclosion in low concentrations (less than 1 microgram/microliter hemolymph). Vitellogenin and vitellin are immunologically identical and are composed of a single type of apo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940170208
更新日期:1991-01-01 00:00:00
abstract::A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was una...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21221
更新日期:2015-05-01 00:00:00
abstract::The Colorado potato beetle (Leptinotarsa decemlineata [Say]) is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lac...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21642
更新日期:2020-01-01 00:00:00
abstract::Plant produced insect molting hormones, termed phytoecdysteroids (PEs), are thought to function as plant defenses against insects by acting as either feeding deterrents or through developmental disruption. In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following r...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.10062
更新日期:2002-12-01 00:00:00
abstract::Two predatory fly species, Lispe consanguinea Loew, 1858 and L. tentaculata DeGeer, 1776, inhabit the supralittoral zone at the shore of a fresh-water reservoir. Both species look alike and possess similar "badges," reflective concave silvery scales on the face. Flies occupy different lek habitats. Males of the first ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20118
更新日期:2006-05-01 00:00:00
abstract::Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940150104
更新日期:1990-01-01 00:00:00
abstract::Recent studies have demonstrated that interaction between various agrochemicals and soil microorganisms may either slow down or enhance processes of degradation. Soil disinfestation is employed for the control of soil-borne pathogens and weeds. Soil application of such broad-spectrum biocides, as well as some more sel...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940220312
更新日期:1993-01-01 00:00:00
abstract::The toxic manipulator of oogenesis (TomO) protein has been identified in the wMel strain of Wolbachia that symbioses with the vinegar fly Drosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21471
更新日期:2018-07-01 00:00:00
abstract::Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules struct...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1997)34:3<313::AID-ARCH6>3
更新日期:1997-01-01 00:00:00
abstract::Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promo...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21083
更新日期:2013-04-01 00:00:00
abstract::V-ATPases are ubiquitous proton pumps found in eukaryotes, and are important in regulating the pH of cell compartments and in creating membrane potentials. The V-ATPase creates a proton gradient that is used as an energy source for the transport of other ions. The 16-kDa proteolipid is the proton-translocating subunit...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/1520-6327(200011)45:3<109::AID-ARCH2>3.0.C
更新日期:2000-11-01 00:00:00
abstract::The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)38:4<185::AID-ARCH4>3
更新日期:1998-01-01 00:00:00
abstract::To determine differential gene expression profiles in the venom gland and sac (gland/sac) of a solitary hunting wasp species, Orancistrocerus drewseni Saussure (1857), a subtractive cDNA library was constructed by suppression subtractive hybridization. A total of 498 expressed sequence tags (EST) were clustered and as...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20316
更新日期:2009-08-01 00:00:00
abstract::Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight bi...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940150403
更新日期:1990-01-01 00:00:00
abstract::Pteromalus puparum is a gregarious pupal endoparasitoid with a wide host range. It deposits eggs into pierid and papilionid butterfly pupae. Glutathione S-transferases (GSTs) are a family of multifunctional detoxification enzymes that act in xenobiotic metabolism in insects. Insect genome projects have facilitated ide...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.21634
更新日期:2020-02-01 00:00:00
abstract::When diapause and non-diapause eggs of the same bivoltine strain of Bombyx mori were chilled at 5 degrees C for more than 30 days, the hatchability of diapause eggs increased while that of non-diapause eggs decreased, respectively. To investigate the relationship between effects of chilling on the hatchability and the...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.20367
更新日期:2010-06-01 00:00:00
abstract::An in vitro bioassay suitable for routine use to investigate hypertrehalosemic hormone (HTH)-dependent trehalose biosynthesis was developed for the cockroach fat body. Blaberus discoidalis fat bodies were isolated and divided so that eight matched pieces from a single tissue could be compared for multiple control and ...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/arch.940280402
更新日期:1995-01-01 00:00:00
abstract::The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viabl...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1998)38:3<147::AID-ARCH5>3
更新日期:1998-01-01 00:00:00
abstract::The polyhydroxy structure of ecdysteroids provides a basis for the formation of various derivatives including the acetonides that have been frequently prepared mainly in preparative work. Acetonides can be formed between vicinal diols in cis position such as positions C-2/C-3 and C-20/C-22 in 20-hydroxyecdysone, for e...
journal_title:Archives of insect biochemistry and physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1520-6327(1999)41:3<162::AID-ARCH8>3
更新日期:1999-01-01 00:00:00