A cadherin-like protein from the beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is a putative Cry1Ac receptor.

Abstract:

:In S. exigua, ingestion of Cry1Ac reduces larval growth, shortens lifespan, and decreases copulation and oviposition of the adults. Cadherin-like protein SeCad1b in S. exigua has recently been published. Here, we tested whether SeCad1b mediates the negative effects of Cry1Ac. We identified three potential Cry toxin binding regions in SeCad1b, i.e., (879) EIAIQITDTNN(889) , (1357) SLLTVTI(1363) , and (1436) GVISLNFQ(1443) . We expressed and purified a truncated cadherin, rSeCad1bp, and its interspecific homologue, rHaBtRp, from H. armigera that contain the putative toxin binding regions. Using a toxin overlay assay, we found that rSeCad1bp specifically binds to biotinylated Cry1Ac in a dose-dependent manner. We also discovered that an addition of rSeCad1bp and rHaBtRp enhances the suppression of larval growth by Cry1Ac, although rSeCad1bp is less suppressive than rHaBtRp. Finally, RNA interference-mediated knockdown of SeCad1b reduced approximately 80% of the target gene and significantly alleviated the negative effect of CrylAc on larval growth. We infer that the  S. exigua SeCad1b is a functional receptor of Cry1Ac.

authors

Chen RR,Ren XL,Han ZJ,Mu LL,Li GQ,Ma Y,Cui JJ

doi

10.1002/arch.21163

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

58-71

issue

1

eissn

0739-4462

issn

1520-6327

journal_volume

86

pub_type

杂志文章
  • Comparative analysis of two biliproteins, BP1 and BP2, from haemolymph of cabbage white butterfly, Pieris rapae.

    abstract::Two blue-pigment binding proteins, BP1 and BP2, are present in larval and pupal haemolymph of cabbage white butterfly, Pieris rapae, and fluctuate in expression during development. Both BP1 and BP2 are found in pupal haemolymph in varying proportions as well as in adult haemolymph, while only small amounts of BP2 are ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20106

    authors: Choi CW,Nam KP,Seo DH,Choi JW,Lee CS,Kim HR,Yun CY

    更新日期:2006-04-01 00:00:00

  • Purification and characterization of arginine kinase from the American cockroach (Periplaneta americana).

    abstract::The isolation and characterization of homogeneous arginine kinase from the cockroach is reported. The purification protocol produces 6.6 mg of pure enzyme from 6.8 g of whole cockroach. The purified enzyme cross-reacts with a heterologous antibody and monoclonal antibody against arginine kinase from the shrimp. Both a...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.10143

    authors: Brown AE,France RM,Grossman SH

    更新日期:2004-06-01 00:00:00

  • Genes acting in longevity-related pathways in the endoparasitoid, Pteromalus puparum.

    abstract::Among insects, lifespans vary over a broad range, from the short-lived mayflies to the 17-year periodical cicadas. Generally, lifespans are determined by a phase in life, the reproductive lifespan, which varies among species. Numerous pathways, such as the insulin/insulin-like growth factor signaling pathway, the targ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21635

    authors: Xiong S,Yu K,Ye X,Fang Q,Deng Y,Xiao S,Yang L,Wang B,Wang F,Yan Z,Wang F,Song Q,Stanley DW,Ye G

    更新日期:2020-02-01 00:00:00

  • Enhancing Nanos expression via the bacterial TomO protein is a conserved strategy used by the symbiont Wolbachia to fuel germ stem cell maintenance in infected Drosophila females.

    abstract::The toxic manipulator of oogenesis (TomO) protein has been identified in the wMel strain of Wolbachia that symbioses with the vinegar fly Drosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21471

    authors: Ote M,Yamamoto D

    更新日期:2018-07-01 00:00:00

  • LARVAL X-RAY IRRADIATION INFLUENCES PROTEIN EXPRESSION IN PUPAE OF THE ORIENTAL FRUIT FLY, BACTROCERA DORSALIS.

    abstract::The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21330

    authors: Chang CL,Goodman CL,Ringbauer J,Geib SM,Stanley D

    更新日期:2016-07-01 00:00:00

  • Isolation and characterization of bacteria-induced protein P4 from hemolymph of Manduca sexta.

    abstract::Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940150104

    authors: Ladendorff NE,Kanost MR

    更新日期:1990-01-01 00:00:00

  • Identification of a putative azadirachtin-binding complex from Drosophila Kc167 cells.

    abstract::Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or wit...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20171

    authors: Robertson SL,Ni W,Dhadialla TS,Nisbet AJ,McCusker C,Ley SV,Mordue W,Mordue 'Luntz' AJ

    更新日期:2007-04-01 00:00:00

  • Plasmatocyte spreading peptide does not induce Microplitis demolitor polydnavirus-infected plasmatocytes to spread on foreign surfaces.

    abstract::Capsule formation by the moth Pseudopulsia includens requires that plasmatocytes change from being nonadhesive cells in circulation to strongly adhesive cells capable of attaching to the foreign target and one another. This change in adhesive state is induced by Plasmatocyte Spreading Peptide (PSP1); a 23 amino acid p...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)40:1<41::AID-ARCH5>3.

    authors: Strand MR,Clark KC,Gardiner EM

    更新日期:1999-01-01 00:00:00

  • Molecular characterization of the ebony gene from the American cockroach, Periplaneta americana.

    abstract::Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20064

    authors: Blenau W,Baumann A

    更新日期:2005-07-01 00:00:00

  • Double-stranded RNAs targeting inhibitor of apoptosis gene show no significant cross-species activity.

    abstract::RNA interference (RNAi) has become an integral part of mainstream research due to its versatility and ease of use. However, the potential nontarget effects associated with double-stranded RNAs (dsRNA) are poorly understood. To explore this, we used dsRNAs targeting the inhibitor of apoptosis (iap) gene from nine insec...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21683

    authors: Chereddy SCRR,Gurusamy D,Howell JL,Palli SR

    更新日期:2020-08-01 00:00:00

  • TWO INNEXINS OF Spodoptera litura INFLUENCES HEMICHANNEL AND GAP JUNCTION FUNCTIONS IN CELLULAR IMMUNE RESPONSES.

    abstract::Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21243

    authors: Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo K

    更新日期:2015-09-01 00:00:00

  • Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual.

    abstract::Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章,评审

    doi:10.1002/arch.21652

    authors: Tsoumani KT,Meccariello A,Mathiopoulos KD,Papathanos PA

    更新日期:2020-03-01 00:00:00

  • Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae.

    abstract::A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanoga...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20339

    authors: Sonoda S,Tsumuki H

    更新日期:2010-01-01 00:00:00

  • Genomic organization of DHR38 gene in Drosophila: presence of Alu-like repeat in a translated exon and expression during embryonic development.

    abstract::The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1998)38:4<185::AID-ARCH4>3

    authors: Komonyi O,Mink M,Csiha J,Maróy P

    更新日期:1998-01-01 00:00:00

  • Serine protease from midgut of Bombus terrestris males.

    abstract::A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These b...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21075

    authors: Brabcová J,Kindl J,Valterová I,Pichová I,Zarevúcka M,Brabcová J,Jágr M,Mikšík I

    更新日期:2013-03-01 00:00:00

  • Farnesoic acid O-methyl transferase (FAMeT) isoforms: conserved traits and gene expression patterns related to caste differentiation in the stingless bee, Melipona scutellaris.

    abstract::Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20224

    authors: Vieira CU,Bonetti AM,Simões ZL,Maranhão AQ,Costa CS,Costa MC,Siquieroli AC,Nunes FM

    更新日期:2008-02-01 00:00:00

  • Starvation influences allatotropin gene expression and juvenile hormone titer in the female adult oriental armyworm, Mythimna separata.

    abstract::The first day of adult life is the sensitive stage for shifting migrants into the resident morphs of the oriental armyworm (OAW), Mythimna separata (Walker). The juvenile hormone (JH) titer, expression of the allatotropin (AT) gene, and their relationship were investigated in adult female migrants starved in the sensi...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20255

    authors: Zhang L,Luo L,Jiang X

    更新日期:2008-06-01 00:00:00

  • Digestive proteinase activity in corn earworm (Helicoverpa zea) after molting and in response to lowered redox potential.

    abstract::Insect digestive proteinases are often strongly influenced by ambient physicochemical conditions, such as pH, ionic strength, and oxidation-reduction potential. Although the effects of the former two parameters are well documented, the influence of redox potential on catalytic rates of digestive enzymes is not well un...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/1520-6327(200008)44:4<151::AID-ARCH2>3.0.C

    authors: Johnson KS,Felton GW

    更新日期:2000-08-01 00:00:00

  • Inga laurina trypsin inhibitor (ILTI) obstructs Spodoptera frugiperda trypsins expressed during adaptive mechanisms against plant protease inhibitors.

    abstract::Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-ind...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21393

    authors: Machado SW,de Oliveira CFR,Zério NG,Parra JRP,Macedo MLR

    更新日期:2017-08-01 00:00:00

  • Phosphorylation of Rab proteins from the brain of Bombyx mori.

    abstract::Rab proteins play fundamental roles in the regulation of membrane traffic. Previously, from the brain of Bombyx mori we isolated two cDNA clones (BRab1 and BRab14), each of which encoded a different member of Rab-protein family and was expressed in Escherichia coli and purified using an affinity chromatography. In thi...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20014

    authors: Uno T,Nakao A,Katsurauma C

    更新日期:2004-10-01 00:00:00

  • Cloning of the 16-kDa V-ATPase proteolipid subunit from the red imported fire ant Solenopsis invicta buren (Hymenoptera: Formicidae).

    abstract::V-ATPases are ubiquitous proton pumps found in eukaryotes, and are important in regulating the pH of cell compartments and in creating membrane potentials. The V-ATPase creates a proton gradient that is used as an energy source for the transport of other ions. The 16-kDa proteolipid is the proton-translocating subunit...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/1520-6327(200011)45:3<109::AID-ARCH2>3.0.C

    authors: Holmes SP,Frazier SK,Pietrantonio PV

    更新日期:2000-11-01 00:00:00

  • HPLC method for the analysis of acetonides of ecdysteroids providing structural information on different vicinal diols

    abstract::The polyhydroxy structure of ecdysteroids provides a basis for the formation of various derivatives including the acetonides that have been frequently prepared mainly in preparative work. Acetonides can be formed between vicinal diols in cis position such as positions C-2/C-3 and C-20/C-22 in 20-hydroxyecdysone, for e...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1999)41:3<162::AID-ARCH8>3

    authors: Kayser H,Eilinger P

    更新日期:1999-01-01 00:00:00

  • Molecular characterization, immunohistochemical localization and expression of a ribosomal protein L17 gene from Apis cerana cerana.

    abstract::Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreove...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.20386

    authors: Meng F,Zhang L,Kang M,Guo X,Xu B

    更新日期:2010-10-01 00:00:00

  • Expression of a parasitism-specific protein in lepidopteran hosts of Chelonus sp.

    abstract::The hemolymph of each noctuid species successfully parasitized by Chelonus near curvimaculatus possessed a parasitism-specific protein (PSP) previously identified in host T. ni (Insect Biochem. 19:445;21:845). Expression of PSP occurred in a stage-specific manner in the stadium during which the host undergoes precocio...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940240305

    authors: Soldevila AI,Jones D

    更新日期:1993-01-01 00:00:00

  • Delayed and enhanced biodegradation of soil-applied diphenamid, carbendazim, and aldicarb.

    abstract::Recent studies have demonstrated that interaction between various agrochemicals and soil microorganisms may either slow down or enhance processes of degradation. Soil disinfestation is employed for the control of soil-borne pathogens and weeds. Soil application of such broad-spectrum biocides, as well as some more sel...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940220312

    authors: Aharonson N,Katan J

    更新日期:1993-01-01 00:00:00

  • Molecular cloning of a cDNA for a small GTP binding protein, BRho, from the embryo of Bombyx mori and its characterization after expression and purification.

    abstract::A cDNA clone encoding a small GTP binding protein (Brho) was isolated from an embryonic cDNA library of Bombyx mori that encoded a polypeptide with 202 amino acids sharing 60-80% similarity with the Rho1 family of GTP binding proteins. The effector site and one of the guanine nucleotide binding sites differed from oth...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(200004)43:4<165::AID-ARCH2

    authors: Uno T,Nakasuji A,Hara W,Aizono Y

    更新日期:2000-04-01 00:00:00

  • Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line.

    abstract::Ecdysteroids, or molting hormones, have been proven to be key differentiation regulators for epidermal cells in the postembryonic development of arthropods. Regulators of cell proliferation, however, remain largely unknown. To date, no diffusible insect peptidic growth factors have been characterized. Molecules struct...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1520-6327(1997)34:3<313::AID-ARCH6>3

    authors: Hatt PJ,Liebon C,Morinière M,Oberlander H,Porcheron P

    更新日期:1997-01-01 00:00:00

  • Cloning of a VLP-protein coding gene from a parasitoid wasp Venturia canescens.

    abstract::Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.940260207

    authors: Theopold U,Krause E,Schmidt O

    更新日期:1994-01-01 00:00:00

  • Insecticidal and synergistic activity of dsRNAs targeting buprofezin-specific genes against the small brown planthopper, Laodelphax striatellus.

    abstract::The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative tra...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.21739

    authors: Fang Y,Park MG,Choi JY,Park DH,Wang M,Kim HJ,Kim WJ,Je YH

    更新日期:2020-12-01 00:00:00

  • Alkaline phosphatase activity in whitefly salivary glands and saliva.

    abstract::Alkaline phosphatase activity was histochemically localized in adult whiteflies (Bemisia tabaci B biotype, syn. B. argentifolii) with a chromogenic substrate (5-bromo-4-chloro-3-indolylphosphate) and a fluorogenic substrate (ELF-97). The greatest amount of staining was in the basal regions of adult salivary glands wit...

    journal_title:Archives of insect biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1002/arch.1026

    authors: Funk CJ

    更新日期:2001-04-01 00:00:00