Alkaline phosphatase activity in whitefly salivary glands and saliva.

abstract：:Insect cellular immune responses include encapsulation, nodule formation, and phagocytosis. Hemichannels and gap junctions are involved in these cellular actions. Innexins (Inxs: analogous to the vertebrate connexins) form hemichannels and gap junctions, but the molecular mechanisms underlying their biology is still u...

journal_title：Archives of insect biochemistry and physiology

authors： Pang Z,Li M,Yu D,Yan Z,Liu X,Ji X,Yang Y,Hu J,Luo K

更新日期：2015-09-01 00:00:00

abstract：:Acromelic acid, a naturally occurring kainoid, isolated from the mushroom Clitocybe acromelalga, is a weak displacer of [3H]L-glutamate binding to cockroach (Periplaneta americana) nerve cord membranes. Acromelic acid (1 mM) displaces approximately 60% of specifically bound [3H]L-glutamate. When applied by bath perfus...

journal_title：Archives of insect biochemistry and physiology

authors： Sattelle DB,Sepúlveda MI,Shinozaki H,Ishida M

更新日期：1994-01-01 00:00:00

abstract：:The glucosidase inhibitor 1,4-dideoxy-1,4-imino-D-arabinitol (DIA) was used to investigate the action of hypertrehalosemic hormone (HTH) on carbohydrate, neutral lipid, and phospholipid metabolism in the hemolymph and fat body of the cockroach, Periplaneta americana. DIA blocked the hypertrehalosemic and hyperglycemic...

journal_title：Archives of insect biochemistry and physiology

authors： Oguri E,Steele JE

更新日期：2006-12-01 00:00:00

abstract：:A protocol has been developed for the purification of vitellogenin from the honey bee, Apis mellifera. Purification allows for the first characterization of a vitellogenin from the large order Hymenoptera. Hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotei...

journal_title：Archives of insect biochemistry and physiology

authors： Wheeler DE,Kawooya JK

更新日期：1990-01-01 00:00:00

abstract：:The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alka...

journal_title：Archives of insect biochemistry and physiology

authors： Nussenzveig RH,Oliveira PL,Masuda H

更新日期：1992-01-01 00:00:00

abstract：:Pheromone titer in moths is a product of three processes occurring in or at the surface of the pheromone gland: biosynthesis, release, and intraglandular degradation, of pheromone. Changes in titers of sex pheromone, the fatty acyl pheromone analog (FAPA), and tetradecanoate, a pheromone biosynthetic intermediate, wer...

journal_title：Archives of insect biochemistry and physiology

authors： Foster SP

更新日期：2000-03-01 00:00:00

abstract：:Apolipophorin III (apoLp-III) functions in lipid transport and immune activation in insects. We cloned a cDNA encoding putative apoLp-III from larvae of Thitarodes pui, a host species of Ophiocordyceps sinensis, with great economic importance in the Tibetan Plateau. Excluding a putative signal peptide of the first 20 ...

journal_title：Archives of insect biochemistry and physiology

authors： Sun Z,Yu J,Wu W,Zhang G

更新日期：2012-06-01 00:00:00

abstract：:The recombinant lambda clone 4-2 containing the genomic region of the Drosophila hormone receptor 38 (DHR 38) gene, homologous to mammalian neuronal growth factor I-B (NGFI-B), was isolated by radioactive labelled oligonucleotide hybridization. The nucleotide sequence of the genomic clone revealed three exons that enc...

journal_title：Archives of insect biochemistry and physiology

authors： Komonyi O,Mink M,Csiha J,Maróy P

更新日期：1998-01-01 00:00:00

abstract：:In previous investigations, we have determined that organophosphate resistance in the western corn rootworm, Diabrotica virgifera virgifera, is at least partially attributed to a group of non-specific carboxylesterases referred to as group II. Antiserum raised against a purified 66-kDa group II esterase is specific fo...

journal_title：Archives of insect biochemistry and physiology

authors： Zhou X,Scharf ME,Meinke LJ,Chandler LD,Siegfried BD

更新日期：2005-03-01 00:00:00

abstract：:Binding sites for vitellogenin were solubilized and analyzed either with a filter assay or with ligand blotting. We tested sodium dodecylsulfate (SDS), Chaps, octyl-beta-D-glucoside, and sodium deoxycholate and found SDS and sodium deoxycholate to be most effective in solubilizing both high and low molecular weight bi...

journal_title：Archives of insect biochemistry and physiology

authors： Indrasith LS,Kindle H,Lanzrein B

更新日期：1990-01-01 00:00:00

abstract：:This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HP...

journal_title：Archives of insect biochemistry and physiology

authors： Wegener C,Predel R,Eckert M

更新日期：1999-01-01 00:00:00

abstract：:This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN), and the use of this antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: a) the content of endogenous PBAN in head extracts of male...

journal_title：Archives of insect biochemistry and physiology

authors： Gazit Y,Dunkelblum E,Ben-Aziz O,Altstein M

更新日期：1992-01-01 00:00:00

abstract：:Insects synthesize several types of hemolymph proteins in response to bacterial infection. The objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of Manduca sexta after injection of bacteria. The protein, isolated by cation exchange and gel filtration chromatography from he...

journal_title：Archives of insect biochemistry and physiology

authors： Ladendorff NE,Kanost MR

更新日期：1990-01-01 00:00:00

abstract：:Many arthropods harbour endosymbiotic bacteria of the genus Wolbachia. These endosymbionts are transmitted vertically from one generation to the next and are obligatory in several Dipterans that have been studied to date. These bacteria induce an array of reproductive isolation mechanisms that are implicated in pest m...

journal_title：Archives of insect biochemistry and physiology

authors： Puttaraju HP,Prakash BM

更新日期：2005-12-01 00:00:00

abstract：:There is a basal level of enzyme activity for trypsin, aminopeptidase, amylase, and lipase in the gut of unfed larval (L6) Spodoptera frugiperda. Trypsin activity does not decrease with non-feeding, possibly because of the low protein levels in plants along with high amino acid requirements for growth and storage (for...

journal_title：Archives of insect biochemistry and physiology

authors： Lwalaba D,Hoffmann KH,Woodring J

更新日期：2010-01-01 00:00:00

abstract：:Genetic engineering of plants using transgenic technology is targeted to enhance agronomic performance or improved quality traits in a wide variety of plant species, and has become a fundamental tool for basic research in plant biotechnology. Constitutive promoters are presently the primary means used to express trans...

journal_title：Archives of insect biochemistry and physiology

authors： Tavva VS,Palli SR,Dinkins RD,Collins GB

更新日期：2007-07-01 00:00:00

abstract：:Two molecular forms of juvenile hormone binding proteins were identified in the larval hemolymph of Bombyx mori by photoaffinity labeling. One form having an Mr of 33 kDa was present constantly in the hemolymph of the third to the fifth instar larvae while the other form having an Mr of 35 kDa was detected in the hemo...

journal_title：Archives of insect biochemistry and physiology

authors： Park CH,Kim HR

更新日期：1996-01-01 00:00:00

abstract：:We have used a parasitoid wasp Drosophila melanogaster system to investigate the relationship between the humoral and cellular immune responses in insects. Expression of the gene encoding diptericin, an antibacterial peptide in various D. melanogaster strains parasitized by several species of parasitoid wasps, was stu...

journal_title：Archives of insect biochemistry and physiology

authors： Benassi V,Coustau C,Carton Y

更新日期：2000-02-01 00:00:00

abstract：:Lepidopteran larvae show a cellular response to invading foreign substances that are larger than hemocytes, for example, parasitoid eggs or larvae. This response is called hemocyte encapsulation and is often accompanied by phenoloxidase (PO)-catalyzed melanization. In the present study, we artificially transplanted en...

journal_title：Archives of insect biochemistry and physiology

authors： Kato Y,Yoshida T,Miura K,Tanaka T,Nakamatsu Y,Ochiai M

更新日期：2014-08-01 00:00:00

abstract：:The relatively little-investigated entomopathogen Conidiobolus coronatus secretes several proteinases into culture broth. Using a combination of ion-exchange and size-exclusion chromatography, we purified to homogeneity a serine proteinase of Mr 30,000-32,000, as ascertained by SDS-PAGE. The purified enzyme showed sub...

journal_title：Archives of insect biochemistry and physiology

authors： Bania J,Samborski J,Bogus M,Polanowski A

更新日期：2006-08-01 00:00:00

abstract：:The effects of tebufenozide and methoxyfenozide on vitellogenin (Vg) synthesis/release in the fat body, translocation in hemolymph, uptake by the ovary, and the expression of the ecdysone receptor (EcR) and its heterodimer partner, ultraspiracle protein (USP) in fat body, were investigated in Cydia pomonella. The resu...

journal_title：Archives of insect biochemistry and physiology

authors： Sun X,Song Q,Barrett B

更新日期：2003-03-01 00:00:00

abstract：:The toxicity of potassium ferrocyanide (PFC) and protective effects of 2,4-dinitrophenol (DNP) under PFC treatment were tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with PFC at concentrations of 1.0 mM and mixtures with DNP in concentrations of 0.50 and 1.25 mM, eithe...

journal_title：Archives of insect biochemistry and physiology

authors： Lozinsky OV,Lushchak OV,Lushchak VI

更新日期：2013-11-01 00:00:00

abstract：:Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreove...

journal_title：Archives of insect biochemistry and physiology

authors： Meng F,Zhang L,Kang M,Guo X,Xu B

更新日期：2010-10-01 00:00:00

abstract：:A neuronal morphological phenotype can be induced in cultured Spodoptera frugiperda insect cells (Sf21) by supplementing serum-containing media with 20-hydroxyecdysone (20-HE) and/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells...

journal_title：Archives of insect biochemistry and physiology

authors： Jenson LJ,Bloomquist JR

更新日期：2015-11-01 00:00:00

abstract：:This study reports the biochemical characterization and comparative analyses of highly active serine proteases in the larval and pupal developmental stages of Aedes aegypti (Linnaeus) using substrate-SDS-PAGE. Zymographic analysis of larval stadia detected proteolytic activity in 6-8 bands with apparent molecular mass...

journal_title：Archives of insect biochemistry and physiology

authors： Mesquita-Rodrigues C,Saboia-Vahia L,Cuervo P,Levy CM,Honorio NA,Domont GB,de Jesus JB

更新日期：2011-04-01 00:00:00

abstract：:Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we ...

journal_title：Archives of insect biochemistry and physiology

authors： Theopold U,Krause E,Schmidt O

更新日期：1994-01-01 00:00:00

abstract：:Antioxidant system, which is composed of multiple gene families, plays a major role in reducing oxidative damage and xenobiotic detoxification in all living organisms. We identified 50 silkworm antioxidant genes from nine gene families based on the assembled genome sequence. A comparative analysis of the antioxidant g...

journal_title：Archives of insect biochemistry and physiology

authors： Shi GQ,Yu QY,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viabl...

journal_title：Archives of insect biochemistry and physiology

authors： Komiya K,Agui N,Mitsuhashi J

更新日期：1998-01-01 00:00:00

abstract：:Farnesoic acid O-methyl transferase (FAMeT) is the enzyme that catalyzes the formation of methyl farnesoate (MF) from farnesoic acid (FA) in the biosynthetic pathway of juvenile hormone (JH). This work reports the cloning, sequencing, and expression of FAMeT gene from the stingless bee Melipona scutellaris (MsFAMeT). ...

journal_title：Archives of insect biochemistry and physiology

authors： Vieira CU,Bonetti AM,Simões ZL,Maranhão AQ,Costa CS,Costa MC,Siquieroli AC,Nunes FM

更新日期：2008-02-01 00:00:00

abstract：:Plant protease inhibitors (PIs) are elements of a common plant defense mechanism induced in response to herbivores. The fall armyworm, Spodoptera frugiperda, a highly polyphagous lepidopteran pest, responds to various PIs in its diet by expressing genes encoding trypsins. This raises the question of whether the PI-ind...

journal_title：Archives of insect biochemistry and physiology

authors： Machado SW,de Oliveira CFR,Zério NG,Parra JRP,Macedo MLR

更新日期：2017-08-01 00:00:00