Picrotoxin is a CYP1A1 inducer in rainbow trout hepatocytes.


:Picrotoxin is a CYP2B inducer in mammals (1). However, in primary cultures of rainbow trout hepatocytes we showed CYP1A1 gene expression to be strongly induced as characterized by 7-ethoxyresorufin-O-deethylase activity, immunoblots of CYP1A1 protein, and Northern blots of CYP1A1 mRNA. Induction of CYP1A1 in these cells appeared to be via the aromatic hydrocarbon (Ah) receptor as indicated by increased levels of Ah receptor in the nuclear extracts of picrotoxin-treated cells. Picrotoxin induction of CYP1A1 was blocked by actinomycin D. Superinduction of CYP1A1 mRNA occurred in cells treated with cycloheximide. Co-incubation of hepatocytes with picrotoxin and a saturating concentration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) resulted in synergistic increases in CYP1A1 gene expression. These results suggest that picrotoxin induction of CYP1A1 is regulated at the level of transcription and may involve an alternative mechanism(s) to that of TCDD.


Sadar MD,Ash R,Andersson TB




Has Abstract


1995-09-25 00:00:00














  • Photoaffinity labeling studies of the rat renal sodium bile salt cotransport system.

    abstract::The uptake of a photolabile taurocholate derivative, (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oyl)-2-aminoethanesulfonate, 7,7-azo-TC, into rat renal brush-border membrane vesicles was stimulated by Na+ and inhibited by taurocholate indicating an interaction with the Na+/bile salt cotransport system. Irra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Burckhardt G,Kramer W,Kurz G,Wilson FA

    更新日期:1987-03-30 00:00:00

  • Metyrapone modulation of tyrosine aminotransferase induction by dexamethasone in cultured hepatocytes.

    abstract::Metyrapone, an inhibitor of cytochrome P-450-dependent monooxygenases, enhanced the induction of tyrosine aminotransferase by dexamethasone in primary cultures of hepatocytes, while it had no effect on the basal level of the enzyme activity in the absence of the hormone. The amplification of the hormonal induction of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tongiani R,Piazzolla S,Paolicchi A

    更新日期:1990-01-30 00:00:00

  • Activation of εPKC reduces reperfusion arrhythmias and improves recovery from ischemia: optical mapping of activation patterns in the isolated guinea-pig heart.

    abstract:UNLABELLED:Pervious biochemical and hemodynamic studies have highlighted the important role of εPKC in cardioprotection during ischemic preconditioning. However, little is known about the electrophysiological consequences of εPKC modulation in ischemic hearts. Membrane permeable peptide εPKC selective activator and inh...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Restivo M,Kozhevnikov DO,Qu YS,Yue Y,Mochly-Rosen D,El-Sherif N,Boutjdir M

    更新日期:2012-09-21 00:00:00

  • The effect of gastric inhibitory polypeptide on intestinal glucose absorption and intestinal motility in mice.

    abstract::Gastric inhibitory polypeptide (GIP) is released from the small intestine upon meal ingestion and increases insulin secretion from pancreatic β cells. Although the GIP receptor is known to be expressed in small intestine, the effects of GIP in small intestine are not fully understood. This study was designed to clarif...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogawa E,Hosokawa M,Harada N,Yamane S,Hamasaki A,Toyoda K,Fujimoto S,Fujita Y,Fukuda K,Tsukiyama K,Yamada Y,Seino Y,Inagaki N

    更新日期:2011-01-07 00:00:00

  • Oxidized low-density lipoprotein stimulates nitric oxide release by rabbit aortic endothelial cells.

    abstract::Incubation of rabbit aortic endothelial cells (RAEC) with oxidized low-density lipoprotein (LDL) for 16 h resulted in stimulation of calcium uptake and increased release of nitric oxide (NO) by these cells. Accompanying inhibition of protein tyrosine phosphatase (PTP) activity in these cells was also observed. Convers...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fries DM,Penha RG,D'Amico EA,Abdalla DS,Monteiro HP

    更新日期:1995-02-06 00:00:00

  • A novel Ca2+/calmodulin-dependent protein kinase lacking autophosphorylation activity in the rabbit heart.

    abstract::We report the discovery, semi-purification and characterization of a novel Ca2+/calmodulin-dependent protein kinase (peak I kinase) using syntide 2 as a substrate from the rabbit heart. In the study of dependence of peak I kinase on the concentration of calmodulin, half-maximal activation was obtained at approx. 2.0 x...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Uemura A,Okazaki K,Takesue H,Matsubara T,Hidaka H

    更新日期:1995-06-15 00:00:00

  • A novel siRNA validation system for functional screening and identification of effective RNAi probes in mammalian cells.

    abstract::Small interfering RNAs (siRNAs) have become the most powerful and widely used gene silencing reagents for reverse functional genomics and molecular therapeutics. The key challenge for achieving effective gene silencing in particular for the purpose of the therapeutics is primarily dependent on the effectiveness and sp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hung CF,Lu KC,Cheng TL,Wu RH,Huang LY,Teng CF,Chang WT

    更新日期:2006-08-04 00:00:00

  • Circular dichroism and Fourier-transform infrared spectroscopic studies on T-cell epitopic peptide fragments of influenza virus hemagglutinin.

    abstract::Epitopic peptides representing the C-terminal (HA1) region of cleaved hemagglutinin of influenza virus from different serotypes were synthesized. Circular dichroism and Fourier-transform infrared spectroscopic data showed that peptides HS2 and HS3 have a predominantly alpha-helical conformation in trifluoroethanol. Re...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Holly S,Majer Z,Tóth GK,Váradi G,Rajnavölgyi E,Laczkó I,Hollósi M

    更新日期:1993-06-30 00:00:00

  • Characterization of in vivo phosphorylation of activin type II receptor.

    abstract::An antiserum was generated against a C-terminal peptide of the mouse activin type II receptor (ActR-II), which specifically immunoprecipitates ActR-II protein transiently expressed in COS-1 cells. Autophosphorylation activity of the ActR-II was examined in [32P]orthophosphate labeled COS-1 cells. The immunoprecipitate...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ichijo H,Yamashita H,ten Dijke P,Eto Y,Heldin CH,Miyazono K

    更新日期:1993-08-16 00:00:00

  • Transcription factor Yin Yang 1 stimulates poly(ADP-ribosyl)ation and DNA repair.

    abstract::Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of ADP-ribose polymers from NAD(+). The function of PARP-1 is related to important nuclear processes including DNA repair and transcription. Previous studies demonstrated a specific physical interaction between PARP-1 and the trans...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Oei SL,Shi Y

    更新日期:2001-06-08 00:00:00

  • Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment.

    abstract::Impaired activity of NK (natural killer) cells has been proposed as a mechanism contributing to viral persistence and chronic infection in hepatitis C (HCV) infection. We aimed to assess the impact of HCV infection on NK cells regarding frequency, subset distribution, and cytotoxic and cytokine secretion functions, as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dessouki O,Kamiya Y,Nagahama H,Tanaka M,Suzu S,Sasaki Y,Okada S

    更新日期:2010-03-05 00:00:00

  • CTCF deficiency causes expansion of the sensory domain in the mouse cochlea.

    abstract::The cochlea in the mammalian inner ear is a sensitive and sharply organized sound-detecting structure. The proper specification of neurosensory-competent domain in the otic epithelium is required for the formation of mature neuronal and sensory domains. Genetic studies have provided many insights into inner ear develo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ma JH,Kim HP,Shin JO

    更新日期:2019-05-14 00:00:00

  • MicroRNA-21 promotes osteogenesis of bone marrow mesenchymal stem cells via the Smad7-Smad1/5/8-Runx2 pathway.

    abstract::Bone marrow mesenchymal stem cells (BMMSCs) are pluripotent stem cells, and the osteogenic differentiation of BMMSCs has been drawing attention for a long time. Bone formation is regulated by numerous molecular and cellular signaling pathways, and the differentiation of BMMSCs is controlled by a well-defined genetic p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li X,Guo L,Liu Y,Su Y,Xie Y,Du J,Zhou J,Ding G,Wang H,Bai Y,Liu Y

    更新日期:2017-11-18 00:00:00

  • A zinc finger protein TZF is a novel corepressor of androgen receptor.

    abstract::Steroid hormones control the transcriptional activity of target genes mediated by intracellular nuclear receptors, and these transcriptional activities are modulated by the combination with coactivators and corepressors. We found in this study that testicular zinc finger protein (TZF) that was a nuclear protein with a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ishizuka M,Kawate H,Takayanagi R,Ohshima H,Tao RH,Hagiwara H

    更新日期:2005-06-17 00:00:00

  • Wnt-independent activation of beta-catenin mediated by a Dkk1-Fz5 fusion protein.

    abstract::An XWnt8-Fz5 fusion protein synergizes with LRP6 to potently activate beta-catenin-dependent signaling. Here, we generated a fusion in which XWnt8 was fused to the N-terminus of LRP6 and show it synergizes with both Fz4 and Fz5 to potently transactivate beta-catenin-dependent Wnt signaling. Based on this, we hypothesi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Holmen SL,Robertson SA,Zylstra CR,Williams BO

    更新日期:2005-03-11 00:00:00

  • Direct inhibition of the expression of cyclin D1 gene by sodium butyrate.

    abstract::In the mouse fibroblasts BP-A31 as well as in the human epidermoid carcinoma cells KB-3-1, both cyclin D1 mRNA and protein contents decreased rapidly during incubation with sodium butyrate. The decrease of cyclin D1 mRNA was not prevented by cycloheximide indicating that protein synthesis is not required for the inhib...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lallemand F,Courilleau D,Sabbah M,Redeuilh G,Mester J

    更新日期:1996-12-04 00:00:00

  • Protein kinase activity of rat brain hexokinase.

    abstract::Hexokinase 1 (HK1) purified from rat brain exhibits protein kinase activity, including autophosphorylation and phosphorylation of histone H2A. This protein kinase activity is observed only in the absence of the HK1 carbohydrate substrate, glucose. Analysis of the ATP-binding domains of the mammalian HK1 protein sequen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Adams V,Griffin LD,Gelb BD,McCabe ER

    更新日期:1991-06-28 00:00:00

  • Hypoxia-induced transcriptional activation of vascular endothelial growth factor is inhibited by serum.

    abstract::Expression of vascular endothelial growth factor (VEGF) by cultured vascular smooth muscle cells was analyzed. Serum and hypoxia had nearly additive actions on VEGF mRNA expression. The function of the VEGF promoter in smooth muscle cells was analyzed using transient luciferase reporter assays. Serum and hypoxia stimu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: D'Angelo G,Ladoux A,Frelin C

    更新日期:2000-01-07 00:00:00

  • Polymorphisms of TNFalpha, IL1beta, and IL1RN genes in chronic obstructive pulmonary disease.

    abstract::It is recognized that genetic factors play a role in the susceptibility to COPD. COPD is characterized by airflow limitation. Chronic inflammation causes small airway disease and parenchymal destruction, leading to the airflow limitation. Polymorphisms in pro-inflammatory cytokine genes may confer a risk for the devel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hegab AE,Sakamoto T,Saitoh W,Nomura A,Ishii Y,Morishima Y,Iizuka T,Kiwamoto T,Matsuno Y,Massoud HH,Massoud HM,Hassanein KM,Sekizawa K

    更新日期:2005-04-22 00:00:00

  • Regulation of ferritin mRNA translation in primary erythroblasts: exogenous c-Kit plus EpoR signaling mimics v-ErbA oncoprotein activity.

    abstract::In general, translation efficiency of ferritin mRNAs is modulated by variations in iron supply. In primary avian erythroblasts undergoing short-term proliferation, however, ferritin heavy chain (ferH) mRNA is repressed at all iron levels. Yet, expression of v-ErbA oncoprotein is sufficient to reinduce ferH mRNA utiliz...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mikulits W,Schranzhofer M,Deiner EM,Beug H,Müllner EW

    更新日期:2000-08-28 00:00:00

  • Immortalization of human myogenic progenitor cell clone retaining multipotentiality.

    abstract::Human myogenic cells have limited ability to proliferate in culture. Although forced expression of telomerase can immortalize some cell types, telomerase alone delays senescence of human primary cultured myogenic cells, but fails to immortalize them. In contrast, constitutive expression of both telomerase and the E7 g...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hashimoto N,Kiyono T,Wada MR,Shimizu S,Yasumoto S,Inagawa M

    更新日期:2006-10-06 00:00:00

  • The role of wogonin in controlling SOCS3 expression in neuronal cells.

    abstract::The mechanism underlying the wogonin-mediated increase in the expression of suppressor of cytokine signaling 3 (SOCS3) is unclear. Promoter deletion assay results revealed that wogonin-induced SOCS3 expression is dependent on the AP-1 consensus sequences and two STAT responsive elements (TTACAAGAA and TTCCAGGAA) in th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nam KW,Chae S,Song HY,Mar W,Han MD

    更新日期:2014-08-08 00:00:00

  • Bicarbonate alters cellular responses in respiration assays.

    abstract::Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respiration in adipocytes an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Krycer JR,Fisher-Wellman KH,Fazakerley DJ,Muoio DM,James DE

    更新日期:2017-08-05 00:00:00

  • Formation of MUC1 metabolic complex is conserved in tumor-derived and normal epithelial cells.

    abstract::MUC1 is abundantly expressed at the surface of epithelial cells in many tissues and their carcinomas. In humans, genetic polymorphism and differential splicing produce isoforms that may contribute to MUC1 participation in protection of the cell surface, modulation of cell-cell interactions, signaling, and metastasis. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Julian J,Carson DD

    更新日期:2002-05-17 00:00:00

  • Ubiquitin-specific protease 14 promotes prostate cancer progression through deubiquitinating the transcriptional factor ATF2.

    abstract::Activating Transcription Factor 2 (ATF2) is a member of the ATF/CREB bZIP family of transcription factors and an oncogene in prostate cancer. ATF2 has been reported to be a critical substrate of the CUL3-SPOP-RBX1 E3 ubiquitin ligase complex and the recurrent somatic mutation of SPOP has been believed to be a key feat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Geng L,Chen X,Zhang M,Luo Z

    更新日期:2020-03-26 00:00:00

  • The role of adenine nucleotide translocation in the energization of the inner membrane of mitochondria isolated from rho + and rho degree strains of Saccharomyces cerevisiae.

    abstract::The energization of rho + and rho degrees isolated mitochondria was measured using a tetraphenylphosphonium selective electrode. In both strains translocase mediated ATP/ADP exchange energization was observed. This energization was more sensitive to uncoupler than that induced by respiration in rho + mitochondria. Thi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dupont CH,Mazat JP,Guerin B

    更新日期:1985-11-15 00:00:00

  • The structure of SecB/OmpA as visualized by electron microscopy: The mature region of the precursor protein binds asymmetrically to SecB.

    abstract::SecB, a molecular chaperone in Escherichia coli, binds a subset of precursor proteins that are exported across the plasma membrane via the Sec pathway. Previous studies showed that SecB bound directly to the mature region rather than to the signal sequence of the precursor protein. To determine the binding pattern of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tang Y,Pan X,Tai PC,Sui SF

    更新日期:2010-03-19 00:00:00

  • Licochalcone-A, a novel flavonoid isolated from licorice root (Glycyrrhiza glabra), causes G2 and late-G1 arrests in androgen-independent PC-3 prostate cancer cells.

    abstract::Licochalcone (LA) is a novel estrogenic flavonoid isolated from PC-SPES composition herb licorice root that was reported to show significant antitumor activity in various malignant human cell lines. To better understand its anti-CaP activities, we have investigated LA-elicited growth control and induction of apoptosis...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fu Y,Hsieh TC,Guo J,Kunicki J,Lee MY,Darzynkiewicz Z,Wu JM

    更新日期:2004-09-10 00:00:00

  • Repression of Smad3 activity by histone demethylase SMCX/JARID1C.

    abstract::SMCX/JARID1C was recently shown to be a histone H3 lysine 4 (H3K4) demethylase. Here, we have identified an SMCX isoform that predominantly resides in the cytoplasm, but still efficiently demethylates trimethylated H3K4. SMCX requires several functional domains for its demethylase activity and is also capable of formi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim TD,Shin S,Janknecht R

    更新日期:2008-02-08 00:00:00

  • Cloning, expression, and in vitro activity of human endostatin.

    abstract::Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. I...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dhanabal M,Volk R,Ramchandran R,Simons M,Sukhatme VP

    更新日期:1999-05-10 00:00:00