Calmodulin-binding proteins of calcium-independent type in rat brain synaptosomal membranes: their localization and properties.

Abstract:

:Some properties of calmodulin(CaM)-binding proteins (CaMBPs) of the Ca(2+)-independent type were investigated in the synaptosomal membrane (SM) from rat brain using the [125I]CaM gel overlay method. When SM was prepared in the presence of Ca2+, Ca(2+)-independent CaM binding was decreased, whereas the Ca(2+)-dependent type was not altered. All Ca(2+)-independent-type CaMBPs were membrane-bound and scarcely present in the soluble fractions. When SM was heat-denatured, the 24/22.5-kDa CaMBPs could no longer be detected by [125]CaM binding and a new component with higher molecular mass (greater than 200 kDa) was shown to bind CaM in a Ca(2+)-independent manner. A possible effect of cAMP- and Ca2+/CaM-dependent phosphorylation on CaM binding was also examined.

authors

Natsukari N,Miwa A,Fujita M

doi

10.1016/0006-291x(92)90543-t

subject

Has Abstract

pub_date

1992-03-16 00:00:00

pages

725-32

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(92)90543-T

journal_volume

183

pub_type

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