Abstract:
:Interstitial fluid flow, critical for macromolecular transport, was recently shown to drive fibroblast differentiation and perpendicular cell and matrix alignment in 3D collagen cultures. Here we explore the mechanisms underlying this flow-induced cell and collagen alignment. Cell and matrix alignment was assessed from 3D confocal reflectance stacks using a Fast Fourier Transform method. We found that human dermal and lung fibroblasts align perpendicular to flow in the range of 5-13 mum/s (0.1-0.3 dyn/cm(2)) in collagen; however, neither cells nor matrix fibers align in fibrin cultures, which unlike collagen, is covalently cross-linked and generally degraded by cell fibrinolysis. We also found that even acellular collagen matrices align weakly upon exposure to flow. Matrix alignment begins within 12 h of flow onset and continues, along with cell alignment, over 48 h. Together, these data suggest that interstitial flow first induces collagen fiber alignment, providing contact guidance for the cells to orient along the aligned matrix; later, the aligned cells further remodel and align their surrounding matrix fibers. These findings help elucidate the effects of interstitial flow on cells in matrices and have relevance physiologically in tissue remodeling and in tissue engineering applications.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Ng CP,Swartz MAdoi
10.1007/s10439-005-9067-3subject
Has Abstractpub_date
2006-03-01 00:00:00pages
446-54issue
3eissn
0090-6964issn
1573-9686journal_volume
34pub_type
杂志文章abstract::Rod photoreceptors express a unique type of noninactivating potassium channels, Kx channels, which play an important role in setting the dark potential and participate in shaping the light response. Biophysical studies of Kx channels are limited. For example, the effects of a conventional blocker of potassium channels...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/b:abme.0000042230.99614.8d
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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doi:10.1007/s10439-015-1543-9
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-012-0562-z
更新日期:2012-09-01 00:00:00
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0349-7
更新日期:2011-09-01 00:00:00
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-016-1716-1
更新日期:2017-03-01 00:00:00
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-9981-x
更新日期:2010-07-01 00:00:00
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-016-1593-7
更新日期:2016-10-01 00:00:00
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
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doi:10.1007/s10439-013-0745-2
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章,评审
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