Abstract:
:Previous research in cellular adhesion has focused primarily on studying isolated cells under conditions where cells do not interact with each other. However, in vivo cells form sheets where both cell-substratum and cell-cell interactions contribute to the overall adhesive behavior. Our understanding of how cell-cell and cell-substratum interactions affect the overall process of cell adhesion in these situations is limited. To address this problem, we developed a systematic approach to evaluate how cell-cell and cell-substratum interactions affect the critical shear stress for detachment for semi-confluent and confluent sheets of cells. Our studies were based on subjecting cultures of adherent cells to a defined hydrodynamic flow in a radial-flow chamber with a gap height of 140 microm. Using phase-contrast microscope imaging and analysis we measured shear-dependent patterns of detachment as a function of the extent of cell confluency. Our results show that the critical shear stress for detachment is maximum at intermediate extents of confluency of 10%-40%. These results have important implications for sodding vascular grafts and tissue engineering.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Peel MM,DiMilla PAdoi
10.1114/1.178subject
Has Abstractpub_date
1999-03-01 00:00:00pages
236-46issue
2eissn
0090-6964issn
1573-9686journal_volume
27pub_type
杂志文章abstract::The study was to explore the effects of BMP-2 gene modified canine bone marrow stromal cells (bMSCs) mediated by a nonviral PEI derivative (GenEscort™ II) in promoting bone formation in vitro and in vivo. Canine bMSCs were cultured and transfected with plasmids containing bone morphogenetic protein-2 gene (pBMP-2) or ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0276-7
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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doi:10.1007/s10439-005-4479-7
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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pub_type: 杂志文章
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journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-018-2041-7
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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journal_title:Annals of biomedical engineering
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