Abstract:
:The binding of dantrolene to rabbit red blood cell (RBC) ghosts and bovine serum albumin as models of receptor sites was studied using fluorescence techniques. Dantrolene upon binding to rabbit RBC ghosts and bovine serum albumin showed fluorescence with emission maxima at 495 and 500 nm, respectively. Dantrolene bound to rabbit RBC ghosts with an association constant of 6.06 X 10(4) M-1 and there were 2.4 X 10(6) dantrolene binding sites per ghost. The binding of dantrolene to bovine serum albumin showed an anomaly with respect to dantrolene concentration. Dantrolene at concentrations of 1.25-3.75 microM bound to bovine serum albumin with an association constant of 1.72 X 10(4) M-1 and there were 1.078 binding sites/mol bovine serum albumin. The higher affinity of dantrolene toward blood cells was confirmed by studying the distribution of dantrolene between blood cells and plasma after a single i.p. injection of dantrolene sodium (3 mg/kg) to albino rabbits. It was found that the concentration of dantrolene in rabbit blood cells was 2.76 times that of plasma.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Dehpour AR,Mahmoudian Mdoi
10.1139/y82-192subject
Has Abstractpub_date
1982-10-01 00:00:00pages
1307-11issue
10eissn
0008-4212issn
1205-7541journal_volume
60pub_type
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更新日期:1976-06-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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