Abstract:
:Electrophysiological studies were performed on in vitro slice preparations of sympathetic ganglia excised from peripherally perfused, brain-dead human donors. The intracellular recordings in 16 neurons showed resting potentials and input resistances mostly in the ranges reported for sympathetic neurons in other mammals. The high input resistances (approximately 29 M omega) can account for the long membrane time constants measured in three neurons (means = 13.9 ms). Spikes that were part of anodal break responses as well as those evoked by current pulse injections were tetrodotoxin sensitive and were more prolonged in duration by tetraethylammonium than by 4-aminopyridine applications. Administrations of isoflurane (0.5-2 minimum alveolar concentrations) by perfusion did not greatly affect the membrane properties, but produced a marked reduction in repetitive spike firing evoked by current pulse injections as well as in the postspike afterhyperpolarizations, suggesting that a sympathetic neurogenic mechanism may contribute to the hypotension observed clinically during isoflurane anaesthesia. These investigations demonstrate for the first time that human sympathetic ganglion neurons can be studied successfully in in vitro preparations, and hence are valuable for direct relevance to the human condition.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Puil E,el-Beheiry H,Spigelman Idoi
10.1139/y88-138subject
Has Abstractpub_date
1988-06-01 00:00:00pages
853-7issue
6eissn
0008-4212issn
1205-7541journal_volume
66pub_type
杂志文章abstract::Between 1 and 7 days of life, the newborn rabbit heart shifts from predominantly using carbohydrates to predominantly using fatty acids as an energy substrate. We therefore used isolated working hearts from 1- or 7-day-old rabbits to determine the effects of fatty acids on myocardial glucose use and the ability of hea...
journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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