Abstract:
:Insulin receptors were measured in cultured Zajdela rat hepatoma cells (ZHC cells), a stable cell line which presents differentiated hepatic functions. The number of sites was 50,000/cell at 2 C, and the dissociation constant for high affinity binding was 1.6 x 10(-10) M. Down-regulation of receptors occurred rapidly when cells were treated with insulin; this process was related to ambient insulin concentrations and led to a decrease in the number of insulin receptors from 50,000 to 30,000/cell. Cycloheximide prevented part of this regulation. When down-regulated cells were incubated in standard medium devoid of insulin, the number of receptor sites gradually increased and attained control values within 7 h; cyclohexamide inhibited this process. Insulin markedly enhanced glycogen synthesis in ZHC cells, with an ED50 of 1.0 x 10(-9) M, leading to an increase in the total cell glycogen content. In addition, the predicted righthand shift of the dose-response curve was observed for insulin-treated cells. These findings provide evidence of insulin-induced receptor regulation in cultured ZHC cells which is related to the biological effect of the hormone on glycogen synthesis.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Capeau J,Flaig-Staedel C,Beck JP,Picard Jdoi
10.1210/endo-111-3-993subject
Has Abstractpub_date
1982-09-01 00:00:00pages
993-1000issue
3eissn
0013-7227issn
1945-7170journal_volume
111pub_type
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