Abstract:
:The PTH activates both adenylate cyclase and a mechanism that increases membrane-associated protein kinase-C (PKC) activity. To define the hormone's PKC activation domain we have used a panel of PTH fragments and ROS 17/2 rat osteosarcoma cells as the target cells. PTH equally and maximally increased PKC activity in ROS 17/2 cell membranes at physiological concentrations between 1-50 pM and 5-50 nM, but not at intermediate concentrations or concentrations above 50 nM. The PKC-stimulating picomolar concentrations of PTH did not stimulate adenylate cyclase in ROS 17/2 cells, while the PKC-stimulating nanomolar concentrations of the hormone did stimulate adenylate cyclase, with an EC50 of 1-2 nM. Very high concentrations of PTH, such as 100 nM, that did not increase membrane PKC activity were still able to maximally stimulate adenylate cyclase. PTH fragments lacking the N-terminal amino acids needed for adenylate cyclase activation increased membrane PKC activity, and the PKC activation domain was found to lie within the 28-34 region of the PTH molecule. This was confirmed by showing that optimally effective picomolar concentrations of the human PTH-(28-34) fragment itself were able to increase membrane-associated PKC activity to the same extent as the optimally effective picomolar concentrations of the intact PTH-(1-84) or the larger PTH-(1-34) or PTH-(3-34) fragments.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Jouishomme H,Whitfield JF,Chakravarthy B,Durkin JP,Gagnon L,Isaacs RJ,MacLean S,Neugebauer W,Willick G,Rixon RHdoi
10.1210/endo.130.1.1727720subject
Has Abstractpub_date
1992-01-01 00:00:00pages
53-60issue
1eissn
0013-7227issn
1945-7170journal_volume
130pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We examined the gene expression of V-1, a novel soluble protein with the cdc10/SWI6 motif, in pseudopregnant rat ovaries. Northern blot analysis on days 1, 5, and 11 of pseudopregnancy revealed an approximately 2-fold increase in the V-1 messenger RNA (mRNA) expression level on day 5 to that on day 1, and no significa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.4.8625920
更新日期:1996-04-01 00:00:00
abstract::We previously characterized the transcriptional activity of the human CRH (hCRH) gene promoter in the mouse anterior pituitary cell line AtT20. Here, we show that phorbolester stimulated through the cAMP response element (CRE), located between -227 and -220 relative to the putative cap site, about 2.6-fold the express...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8380380
更新日期:1993-01-01 00:00:00
abstract::Prolactin (Prl) secreting cells in a mixed pituitary cell culture form microscopically-identifiable plaques (zones of hemolysis around the lactotropes) when incubated in a monolayer with staphylococcal protein-A-coated ovine erythrocytes in the presence of Prl antiserum and complement. Plaques form first at 15-30 min ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-1135
更新日期:1983-03-01 00:00:00
abstract::In seasonal species, various physiological processes including reproduction are organized by photoperiod via melatonin, but the mechanisms of melatonin action are still unknown. In birds, the peptide gonadotropin-inhibiting hormone (GnIH) has been shown to have inhibitory effects on reproductive activity and displays ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0848
更新日期:2008-03-01 00:00:00
abstract::Steroid hormone synthesis is a vital function of the adrenal cortex, serves a critical role in gonadal function, and maintains pregnancy if normally executed in the placenta. The substrate for the synthesis of all steroid hormones is cholesterol, and its conversion to the first steroid, pregnenolone, by the cholestero...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0541
更新日期:2009-02-01 00:00:00
abstract::Many aspects of reproductive function are corrected in hypogonadal mice with preoptic area grafts (HPG/POA). Gonadotropin release and gonadal development are dependent on the presence of GnRH cells within the grafts and GnRH innervation of the median eminence. This study examined the effect of a known modulator of GnR...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2432
更新日期:1991-05-01 00:00:00
abstract::Ghrelin, a novel GH-releasing acylated peptide, was recently isolated from rat stomach. It stimulated the release of GH from the anterior pituitary through the GH secretagogue receptor (GHS-R). Ghrelin messenger RNA and the peptide are present in rat stomach, but its cellular source has yet to be determined. Using two...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7757
更新日期:2000-11-01 00:00:00
abstract::To begin to assess the function of the cell cycle regulator cyclin D3 during gametogenesis, the present study examined its expression, interacting partners, and associated kinase activity in the murine testis and ovary. In the early stages of postnatal testicular development, cyclin D3 protein was detected in spermato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.6.6756
更新日期:1999-06-01 00:00:00
abstract::Diabetic cardiomyopathy is characterized by myocyte loss and myocardial fibrosis, leading to decreased elasticity and impaired contractile function. The study examines the downstream signaling whereby oxidative stress, induced by hyperglycemia, leads to myocardial fibrosis and impaired contractile function in the left...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0877
更新日期:2008-01-01 00:00:00
abstract::The study of epigenetics allows for the understanding of gene × environmental interactions and provides a mechanism by which brief internal or external environmental changes can shape lasting differences in gene function and behavior. Epigenetic processes appear to impact a wide variety of physiological processes with...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2010-0793
更新日期:2011-02-01 00:00:00
abstract::Smoltification is a transformation that occurs in some species of salmon, during which solitary fish in fresh water become schooling fish and migrate to the sea. This process is accompanied by large increases in plasma T4. T4 secretion rate and other parameters of T4 metabolism in juvenile coho salmon were estimated b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-399
更新日期:1984-07-01 00:00:00
abstract::Prolactin (PRL) shares several characteristics with growth factors and cytokines, many of which are known to bind to heparan sulfate proteoglycans. In this study we examined the heparin-binding properties of selected members of the PRL/GH family, using heparin affinity columns followed by gel electrophoresis/Western b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6677
更新日期:1999-02-01 00:00:00
abstract::A 28,000 mol wt vitamin D-dependent calcium-binding protein (CaBP), first isolated from avian intestine, has recently been shown to exist in kidney and other tissues. To study the mechanism regulating the production of renal CaBP, we used primary cultures of chick kidney cells as an in vitro model. Renal cortical tubu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-894
更新日期:1987-03-01 00:00:00
abstract::We examined the effects of the polycationic antibiotic, neomycin, on the function of dispersed bovine parathyroid cells. Neomycin caused a reversible, dose-dependent inhibition of low calcium (Ca++)-stimulated PTH release, with half-maximal inhibition at 30 microM. Maximal inhibition (with 200 microM neomycin) was not...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3047
更新日期:1991-06-01 00:00:00
abstract::Although GH plays a key role in postnatal growth and differentiation, its role in fetal differentiation is not clear at the present. The aim of the present study was to investigate whether GH plays a role in fetal sexual differentiation, and we used in vitro organ culture assay of sexual differentiation to determine t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.9.8756530
更新日期:1996-09-01 00:00:00
abstract::Involvement of ionized cytosolic calcium ([Ca2+]i) and protein kinase-C (PKC) in GnRH-stimulated LH release was assessed by correlating measurable changes in [Ca2+]i and LH release in PKC-depleted and nondepleted gonadotropes. Primary cultures of anterior pituitary cells were loaded with the calcium-sensitive fluoresc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547730
更新日期:1992-04-01 00:00:00
abstract::GH promptly increases cytosolic free calcium ([Ca2+]i) in freshly isolated rat adipocytes. Adipocytes deprived of GH for 3 h or longer are incapable of increasing [Ca2+]i in response to GH, but instead respond in an insulin-like manner. Insulin blocks the GH-induced increase in [Ca2+]i in GH-replete cells and stimulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7310
更新日期:2000-02-01 00:00:00
abstract::Thyroid hormone produces a prompt, plasma membrane-mediated increase in several metabolic functions in the rat thymocyte. These effects of thyroid hormone require calcium and are inhibited by the beta-adrenergic antagonist alprenolol. In the present study, the interrelationship between thyroid hormone and adrenergic a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2240
更新日期:1988-05-01 00:00:00
abstract::The present study was designed to investigate the effect of PTH on calcium (Ca2+) transport through the luminal membrane of proximal and distal rabbit tubule segments. Proximal tubule and distal tubule segment suspensions were incubated with the hormone, and the luminal membranes were subsequently purified. Incubation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.7506210
更新日期:1994-01-01 00:00:00
abstract::The effects of recombinant human insulin-like growth factor I (IGF-I) on both vasoactive intestinal peptide (VIP) and PRL production and gene expression were studied using rat anterior pituitary cell cultures grown in serum-free defined medium. We also examined whether pituitary VIP could be involved in the PRL respon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988440
更新日期:1994-12-01 00:00:00
abstract::The effects of dopamine (DA) on voltage-dependent Ca2+ currents were investigated in cultured rat lactotroph cells using the patch clamp recording technique. Each recorded cell was identified by the reverse hemolytic plaque assay. In the whole-cell configuration, two types of Ca2+ currents, L and T, were characterized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-990
更新日期:1990-09-01 00:00:00
abstract::UMR106-06 cells predominantly express the C1a isoform of the rat calcitonin (CT) receptor (CTR). We have compared the homologous regulation of the C1a CTR endogenously expressed in UMR106-06 cells with the cloned C1a CTR in transfected HEK 293 cells, in which expression is driven by a heterologous promoter. It was fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895320
更新日期:1996-11-01 00:00:00
abstract::PTH-related protein (PTHrP) has been shown to be a major factor responsible for hypercalcemia of malignancy. PTHrP acts via the PTH/PTHrP receptor, and therefore, PTH antagonists might be expected to reverse the hypercalcemia in malignancy. In the present studies, the PTH antagonists [Tyr34]bovine (b) PTH-(7-34)NH2, [...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156920
更新日期:1994-05-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) blocks LH-stimulated androstenedione production by immature rat theca-interstitial cells (TIC) in vitro. The mechanism for TNF inhibition of LH-induced androstenedione is unknown and was investigated. LH stimulation of androstenedione synthesis in TIC is mediated via a cAMP-dependent ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404680
更新日期:1993-11-01 00:00:00
abstract::In the rat ovary, follicle cells produce and respond to activin, but as yet, the functional significance of the autocrine/paracrine effects of ovarian activin remains equivocal. To assess the effects of activin on folliculogenesis, normal cycling female rats were injected once every 8 h over a 40-h period with recombi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588210
更新日期:1995-11-01 00:00:00
abstract::Insulin and insulin-like growth factors (IGFs) stimulate responses in skeletal muscle that include effects on carbohydrate and fat metabolism, protein turnover, growth, and differentiation. To gain insight into the relative importance of insulin and IGFs at different stages of development, the expression of their spec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-1064
更新日期:1989-02-01 00:00:00
abstract::Estrogen-based hormone therapy (HT) in postmenopausal women may reduce the risk of Alzheimer's disease (AD), although HT remains controversial. One key concern with HT is the potential of adverse outcomes such as breast and uterine cancer. A promising strategy to maximize HT benefits and minimize HT risks is the use o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1346
更新日期:2008-05-01 00:00:00
abstract::2,5-Hexanedione (2,5-HD) exposure in the rat produces irreversible testicular atrophy, a model of human male infertility that can be used for mechanistic and therapeutic studies. Following testicular injury by 2,5-HD, stem cell factor (SCF), a Sertoli cell-derived growth factor that binds the c-kit receptor on spermat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5678
更新日期:1998-01-01 00:00:00
abstract::3-Hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) reductase activity and reductase mRNA level were determined in adrenals from hamsters treated with ACTH, with or without cycloheximide or aminoglutethimide. Both reductase activity and reductase mRNA level were similarly enhanced by ACTH administration compared to levels...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-158
更新日期:1989-07-01 00:00:00
abstract::Estrogen or raloxifene (LY156758) prevent estrogen deficiency-induced bone loss in animals and humans. We demonstrated in the rat that a 22% reduction in bone mineral density generated by ovariectomy was associated with a 2-fold reduction of transforming growth factor-beta 3 (TGF beta 3) messenger RNA expression in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612550
更新日期:1996-05-01 00:00:00