Abstract:
:Although the main role of prolactin (PRL) in pregnant rodents is to sustain progesterone production by the corpus luteum, progesterone treatment of PRL or PRL receptor (PRL-R) null mice is unable to prevent fetal loss. We have previously shown that the rat decidua is a site of PRL production and action. In this report, we examined the hypothesis, using PRL null mice and rat decidual cell culture, that the absence of this hormone leads to the expression in the decidua of genes detrimental to pregnancy. The results show that decidual growth is normal in PRL null mice treated with PRL, progesterone, or their combination. However, the decidua of mice treated with progesterone starts expressing IL-6 and 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD), two proteins absent from the decidua of wild-type mice and involved, respectively, in inflammation and progesterone catabolism. The expression of both IL-6 and 20alpha-HSD is prevented by PRL treatment. Our results further suggest that PRL inhibition of 20alpha-HSD expression is at the level of transcription and that decidual PRL (dPRL) inhibits 20alpha-HSD promoter activity. Inhibitors of Janus kinase 2 (Jak2) but not other kinases prevent dPRL down-regulation of the 20alpha-HSD promoter. Furthermore, cotransfection of the 20alpha-HSD promoter with expression vectors of constitutively active PRL-R, Jak2, or signal transducer and activator of transcription 5b (Stat5b) leads to substantial inhibition of promoter activity. Taken together, our investigation provides an explanation for the inability of progesterone to sustain pregnancy in PRL null mice and suggests that dPRL plays an important role in pregnancy by repressing the expression of IL-6 and 20alpha-HSD in the decidua. The study also demonstrates that PRL signals through the Jak2/Stat5 pathway to down-regulate 20alpha-HSD expression in the decidua.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Bao L,Tessier C,Prigent-Tessier A,Li F,Buzzio OL,Callegari EA,Horseman ND,Gibori Gdoi
10.1210/en.2006-1643subject
Has Abstractpub_date
2007-05-01 00:00:00pages
2326-34issue
5eissn
0013-7227issn
1945-7170pii
en.2006-1643journal_volume
148pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We studied the interactions of two natural thyroid hormone receptor (TR) mutants from patients with resistance to thyroid hormone (RTH) and an artificial TR mutant with a nuclear receptor corepressor, N-CoR, and a steroid receptor coactivator, SRC-1. In electrophoretic mobility shift assays, wild-type TRbeta-1 interac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.10.6218
更新日期:1998-10-01 00:00:00
abstract::The formation of ovarian follicles and subsequent development after birth are critical processes for female reproduction, and inappropriate coordination of these processes contributes to ovarian pathologies, such as premature ovarian failure and infertility. Identification and functional investigation of the factors i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0797
更新日期:2011-03-01 00:00:00
abstract::The influence of LY117018 and tamoxifen on established uterotropic influence of estradiol was examined in immature ovariectomized rats. When LY117018 was introduced on the fourth day of estradiol treatment, it regressed the uterotropic effect of estradiol. However, tamoxifen did not attenuate estradiol activity under ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-3-987
更新日期:1981-09-01 00:00:00
abstract::It has previously only been possible to assess osteoclastic bone resorption in intact bone, where other cell types may modify or mediate osteoclastic responses to environmental agents. We have recently developed techniques which enable us to measure bone resorption by osteoclasts extracted from bone and have used thes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-234
更新日期:1985-01-01 00:00:00
abstract::Exposure to environmental toxicants during fetal development alters gene expression and promotes disease later in life. Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer widely used for the manufacturing of consumer products. Exposure to DEHP has been associated with obesity, asthma, and low T levels. In utero expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1436
更新日期:2015-01-01 00:00:00
abstract::Steroid hormones can differentially modify gonadotropin release stimulated by GnRH. Decreased GnRH-mediated gonadotropin release has been observed in vitro after pretreatment with androgens or glucocorticoids. In this study, we tested this phenomenon further with the use of a new cytochemical stain for a potent biotin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-396
更新日期:1985-07-01 00:00:00
abstract::The androgen receptor (AR) in Sertoli cells mediates the actions of testosterone on spermatogenesis. However, the transcription factors responsible for AR gene regulation in Sertoli cells remain unknown. In this study, we determined that nuclear factor-kappaB (NF-kappaB) regulates transcription of AR in primary cultur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0987
更新日期:2004-02-01 00:00:00
abstract::IGFs (IGF-I and IGF-II) are essential for development, and their bioactivities are tightly regulated by six related IGF-binding proteins (IGFBPs). IGFBP-5 is the most highly conserved binding protein and is expressed in several key developmental lineages as well as in multiple adult tissues including the mammary gland...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0041
更新日期:2007-05-01 00:00:00
abstract::Impairments in the estradiol (E2)-induced surge of LH occur as aging female rodents lose reproductive cycles. These and other impairments can be substantially attenuated by ovariectomy of the young adult. In female rats, plasma PRL tends to increase with age, whereas in male rats, GH tends to decrease; PRL is regulate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-813
更新日期:1985-02-01 00:00:00
abstract::The adrenal zona glomerulosa (ZG) secretes aldosterone to regulate sodium balance. Chronic sodium restriction increases aldosterone accompanied by ZG expansion. The ZG is innervated by sympathetic, vasoactive intestinal polypeptide (VIP) and neuropeptide tyrosine (NPY), and sensory, calcitonin gene-related peptide, ne...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0487
更新日期:2010-10-01 00:00:00
abstract::Telomerase activity is essential for protection of cells against the telomere erosion that occurs with each round of cell replication, and thus appears to play a role in the indefinite replication potential of some, but not all, eukaryotic cells. In this regard, some tissues contain stem cells that have a long prolife...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5839
更新日期:1998-03-01 00:00:00
abstract::Transforming growth factor-alpha (TGF alpha) has been localized to the anterior pituitary, specifically to the lactotroph and somatotroph cell populations, by our previous studies. Since pituitary lactotrophs are known to undergo growth in response to estrogens, we have used an estradiol-induced pituitary hyperplasia/...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1534540
更新日期:1992-06-01 00:00:00
abstract::Oxytocin (OT) is released by the posterior pituitary during male orgasm and is supposed to participate in the ejaculatory process. We now report evidence demonstrating the presence of an OT receptor gene (real-time RT-PCR and Northern blot) and protein (immunohistochemistry, Western blot, and binding studies) expressi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0962
更新日期:2004-04-01 00:00:00
abstract::The disappearance of 125-I-ACTH from the circulation of 4 pregnant and fetal sheep has been followed after a single injection to the ewe or fetus. The mean metabolic clearance rate for the fetus and the ewe was 55 and 34 ml/min/kg respectively, giving a half-life in each case of about 1 min. The higher fetal than mate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-1-231
更新日期:1975-01-01 00:00:00
abstract::We investigated the effect of central serotonin (5-hydroxytryptamine, 5-HT) administration on hypothalamic tuberoinfundibular dopamine neurons and related changes in neuronal activity to circulating PRL levels in two physiological models: 1) pregnant rats expressing (day 8) or not expressing (days 11 and 16) PRL surge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1359960
更新日期:1992-12-01 00:00:00
abstract::The intermediary role of the putative inhibitory regulatory membrane protein "Ni" in the "antigonadal" activity of the neurohypophysial hormones was investigated in vitro with the use of a primary culture of rat testicular cells. To this end, use was made of the pertussis toxin (PT) probe, an exotoxin of Bordetella pe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-839
更新日期:1984-08-01 00:00:00
abstract::Hypoxia plays a vital role in the progression of endometriosis. Additionally, integrin-mediated aberrant adhesion is also essential for establishment of endometriotic lesions. In this study, we sought to determine the function of hypoxia in integrin-mediated adhesion of endometrial stromal cells (ESCs) in endometriosi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03227
更新日期:2018-04-01 00:00:00
abstract::Our research programs required the preparation of hypophysectomized and orchidectomized rhesus monkeys. This afforded us the possibility to characterize and compare levels of the gonadotropin and inhibin subunit mRNAs in pituitaries from intact and castrate monkeys. Eighteen adult male monkeys, four of which had been ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1537290
更新日期:1992-03-01 00:00:00
abstract::The effects of alloxan-induced diabetes on uterine decidualization and the associated changes in uterine blood flow (UBF) and corpus luteum activity were evaluated in rats between days 4-9 of pseudopregnancy (day 0 = ovulation). Rats were made diabetic (D) with a 40 mg/kg injection (iv) of alloxan on day 1 of pseudopr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-665
更新日期:1988-02-01 00:00:00
abstract::The actions of IGF-I and IGF-II are thought to be largely due to their activation of the IGF-I receptor. However, IGF-II can also bind with high affinity to, and effectively activate, an isoform of the insulin receptor (IR-A) that lacks a sequence at the carboxyl-terminal end of the extracellular alpha subunit due to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0736
更新日期:2006-02-01 00:00:00
abstract::Exercise has a potent insulin-sensitivity enhancing effect on skeletal muscle, but the intracellular mechanisms that mediate this effect are not well understood. In muscle, Ras-related C3 botulinum toxin substrate 1 (Rac1) regulates both insulin- and contraction-stimulated glucose transport and is dysregulated in insu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1220
更新日期:2016-08-01 00:00:00
abstract::Studies were carried out to determine the origin of the immediate increase in plasma calcium following acute calcitonin deficiency in mature rats. Animals were pre-labelled with 45calcium 24 hours and 4 weeks before thyroparathyroidectomy (TPTX) and 1 hour, 24 hours, 2 and 4 weeks before nephrectomy and TPTX and bled ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-2-534
更新日期:1976-02-01 00:00:00
abstract::Bisphenol A (BPA) is a ubiquitous endocrine-disrupting chemical. Developmental exposure produces changes in behavior and gene expression in the brain. Here, we examined social recognition behaviors in mice from the third familial generation (F3) after exposure to gestational BPA. Second-generation mice were bred in on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00196
更新日期:2019-08-01 00:00:00
abstract::We and others have recently shown that 1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] significantly inhibits cell proliferation and increases secretion of prostate-specific antigen (PSA) in LNCaP cells, an androgen-responsive human prostate cancer cell line. The present study was designed to investigate the possible int...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5328
更新日期:1997-08-01 00:00:00
abstract::In the present study epididymal epithelium was immortalized in transgenic mice by expressing simian virus 40 T antigen under a 5.0-kb mouse glutathione peroxidase 5 promoter (GPX5-Tag1). Epididymal tumorigenesis was associated with an increase in c-Myc expression, and a marked decrease in B-Myc expression, with a 500-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0831
更新日期:2004-01-01 00:00:00
abstract::The present work extends our previous report that the intracerebroventricular (i.c.v.) injection of interleukin-1beta(IL-1beta, 80 ng) significantly blunted the testosterone response to 1 U/kg human CG (hCG), an effect that we attributed to the stimulation of inhibitory pathways connecting the hypothalamus to the test...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.7.6090
更新日期:1998-07-01 00:00:00
abstract::The effects of retinoic acids (RAs) on development of seminal vesicles (SVs) of neonatal mice were investigated in vitro. SVs from 0-day-old male mice were cultured for 2-6 days in serum-free, chemically defined medium containing transferrin and BSA supplemented with 5alpha-dihydrotestosterone (DHT; 10(-8) M) and insu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.7.8770910
更新日期:1996-07-01 00:00:00
abstract::IGF1-a small, single-chain, secreted peptide in mammals-is essential for normal somatic growth and is involved in a variety of other physiological and pathophysiological processes. IGF1 expression appears to be controlled by several different signaling mechanisms in mammals, with GH playing a key role by activating an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00259
更新日期:2018-06-01 00:00:00
abstract::Estradiol is known to inhibit antigen presentation in the vagina. We report here that TGFbeta mediates the action of estradiol on vaginal antigen presenting cells (APC). When vaginal APC from ovariectomized rats were incubated with increasing concentrations of TGFbeta1 and TGFbeta2 in the presence of ovalbumin-specifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.8.8938
更新日期:2002-08-01 00:00:00
abstract::In muscle, physiologic hyperinsulinemia, presumably acting on endothelial cells (ECs), dilates arterioles and regulates both total blood flow and capillary recruitment, which in turn influences glucose disposal. In cultured ECs, however, supraphysiological (e.g. >or=10 nM) insulin concentrations are typically used to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0505
更新日期:2005-11-01 00:00:00