Abstract：
:Hybridomas producing monoclonal antibodies that bind to determinants on myelogenous leukaemia blast cells were developed using fresh leukaemia blasts as immunogens. These monoclonal antibodies were used to quantitate the amount of a variety of antigens on both leukaemia and normal blood cells in a radioimmunoassay. The ability of these antibodies to mediate complement-dependent lysis of leukaemia cells and normal blood cells was evaluated. Significant quantitative differences in the expression of a variety of cell surface antigens were observed among different myeloid leukaemia cell samples, normal cells, and other forms of leukaemia. However, none of the monoclonal antibodies studied were found to be specific for myeloblasts. Despite the fact that these antibodies bound to both leukaemia and normal cells, several of them mediated complement-dependent cytotoxicity which was restricted to leukaemic myeloblasts (AML-1-211, AML-2-30, CML-18, CML-75, CML-115, and CML-150). None of these clones, alone or in any combination, were capable of lysing normal lymphocytes or monocytes. Others were specifically cytotoxic to leukaemia cell lines (AML-1-99), B cells and some leukaemia samples (AML-2-9), and myelomonocytic leukaemia cell samples and normal monocytes (AML-2-23). Several of the monoclonal antibodies from this panel appear to be promising for future clinical applications.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Ball ED,Fanger MWsubject
Has Abstractpub_date
1982-06-01 00:00:00pages
655-65issue
3eissn
0009-9104issn
1365-2249journal_volume
48pub_type
杂志文章abstract：:Activation of human peripheral blood lymphocytes from unsensitized donors by fractions of T.b rhodesiense has been demonstrated in vitro. Both T and B cells were stimulated to differentiate into blast cells. Purified T cells failed to proliferate, but did so when mitomycin C-treated non-T cells were added. Non-T cell ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:Immune complexes (IC) were detected and isolated from the serum of Brown Norway (BN), (Lewis x BN)F1, and Lewis rats bearing a Moloney sarcoma (MST). IC were isolated from the serum of individual rats employing a system of G-200 chromatography and passage through a heavy chain specific anti-rat IgG Immunoadsorbent. Ig...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and paired peripheral blood samples was compared using a polymerase chain reaction (PCR)-based assay. Eight rheumatoid arthritis (RA) patients who had varying durations of disease (from 2 to 20 years) were studied. In all pa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb05865.x
更新日期：1992-12-01 00:00:00
abstract：:The number of thymic epithelial cells containing the serum thymic factor (FTS or thymulin), assessed by indirect immunofluorescence using an anti-FTS monoclonal antibody, was studied in the thymus of normal and autoimmune mice as a function of age. In normal mice the number of FTS+ cells was constant until the age of ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:Several immunological tests were performed on eighty-five patients with rubella. There was a decrease in total leucocytes, neutrophils and T cells, an increase in IgG and the complement components of C4, C9 and CH50, and a marked insensitivity to dinitrochlorobenzen (DNCB) and purified protein derivative (PPD) in many...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:Specific assays for 5'-nucleotidase, adenosine diphosphatase (ADPase) and Mg2+-dependent adenosine triphosphatase (Mg2+-ATPase) have been optimized for human lymphocytes and their subcellular localizations determined by sucrose density gradient centrifugation. 5'-Nucleotidase was localized solely to the plasma membran...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:Ultrastructural study of mucosal eosinophils in a case of eosinophilic gastroenteritis involving stomach, duodenum and ileum showed an altered structure in ulcerated duodenal areas. The electron core density of eosinophil granules was inverted or disappeared and tubulovesicular structures occurred. Using immunogold st...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Wiskott-Aldrich syndrome (WAS) is an X-linked platelet/immunodeficiency disease. The affected gene encodes WASP, a multidomain protein that regulates cytoskeletal assembly in blood cells. Patients have recurring infections, and their lymphocytes exhibit deficient proliferative responses in vitro. We report an evaluati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02409.x
更新日期：2004-04-01 00:00:00
abstract：:Treatment of systemic infection with Candida albicans with a combination of an antifungal agent (i.e. fluconazole) and a thymus-derived immunostimulant (i.e. thymosin alpha 1 (T alpha 1)) in mice immunosuppressed by morphine treatments was investigated. In normal mice, fluconazole given after infection with 10(6) C. a...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06093.x
更新日期：1994-09-01 00:00:00
abstract：:Lymphocyte subpopulations in thirty-nine patients with the tropical splenomegaly syndrome (TSS) were studied using the E-rosette, EAC rosette and direct immunofluorescent staining techniques. In the peripheral blood a decrease in the percentage of T-lymphocytes was found which was accompanied by an increase in the per...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
abstract：:A method for the isolation of complement-fixing immune complexes from human serum and the separation of antigen from antibody is described. In order to isolate the complexes, we used soluble bovine conglutinin in a three-step procedure: (1) serum containing immune complexes is reacted with conglutinin in the presence ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:Isoelectric focusing of serial bleeds from patients with Hashimoto's thyroiditis was carried out and thyroglobulin (Tg) autoantibodies visualized using 125I-labelled Tg followed by autoradiography. Although the spectrotype of these antibodies was polyclonal and varied from patient to patient, each individual's spectro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:Anticardiolipin antibodies (ACA) belong to a heterogeneous group of antibodies directed against negatively charged phospholipids. In patients with rheumatic disorders, their presence has been correlated to the occurrence of thromboembolic complications, thrombocytopenia, abortions and other disease manifestations. Sev...
journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1365-2249.1994.tb05498.x
更新日期：1994-12-01 00:00:00
abstract：:One of the hallmarks of coeliac disease, both active and treated, is an increased number and proportion of gamma/delta intraepithelial T lymphocytes in the small intestinal mucosa, and an increased number of gamma/delta T cells in the small intestinal mucosa of coeliac disease patients has been associated with the inh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.d01-887.x
更新日期：1997-01-01 00:00:00
abstract：:Chlamydia pneumoniae infection is associated with atherosclerosis and the organism has been identified in arterial lesions. To determine whether T lymphocyte-mediated immune responses to Chlamydia antigens within plaque could contribute to pathogenesis, we have derived T cell lines from atherosclerotic plaques of 32 p...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01294.x
更新日期：2000-08-01 00:00:00
abstract：:YKL-40 is secreted by macrophages and neutrophils and patients with bacterial infections have elevated circulating YKL-40. The aim was to evaluate changes in plasma YKL-40 (determined by enzyme-linked immunosorbent assay (ELISA) at 0, 2, 4, 8, 24 and 32 h) in eight healthy volunteers after injection with Esherichia co...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02763.x
更新日期：2005-05-01 00:00:00
abstract：:To investigate the role of seminal plasma in human B cell functions, its effect on the proliferation and antibody secretion of tonsillar B cells and an Epstein-Barr virus (EBV) transformed human B cell line, A4, was examined. Seminal plasma inhibited both the proliferation and differentiation of normal B cells only wh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05700.x
更新日期：1991-07-01 00:00:00
abstract：:Staphyllococcus aureus-induced infections often result in high mortality and permanent joint destruction, despite treatment with antibiotics. IL-10 is typically regarded as an anti-inflammatory cytokine because it promotes a T helper cell type 2 response, and subsequently down-regulates cell mediated immune functions....
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01999.x
更新日期：2002-12-01 00:00:00
abstract：:Significant levels of EAC-rosette inhibition compared to control subjects were found in the sera of patients with focal and segmental hyalinosis (FSH), membranoproliferative glomerulonephritis (MPGN) and extra-membranous glomerulonephritis (EGN). In patients with IgA disease, although some sera produced high levels of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:Bacille Calmette-Guérin (BCG), developed a century ago, is the only licensed tuberculosis (TB) vaccine in use to date. The protective efficacy of BCG against TB varies with no apparent protection in some population, and mechanisms of its immune protection is poorly known, and yet BCG is the most widely used vaccine, w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2012.04614.x
更新日期：2012-09-01 00:00:00
abstract：:Atopic disorders are caused by disregulated activation of T helper 2 (Th2) cells that produce IL-4 and IL-5. Because the presence of IL-4 potently augments the differentiation of naive T cells into Th2 cells, it is important to seek the cell population which provides IL-4 for naive T cells. Recently, a unique subpopul...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01157.x
更新日期：2000-03-01 00:00:00
abstract：:Anti-phospholipid syndrome (APS) is characterized by arterial and/or venous thrombosis and pregnancy morbidity. It is well known that in these patients thrombosis may be the result of a hypercoagulable state related to anti-β2-glycoprotein I (β2-GPI) antibodies. Moreover, platelets may play a role in thrombotic manife...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13248
更新日期：2019-04-01 00:00:00
abstract：:T cells of C57BL/6J (B6) mice activated in vitro against the K and D determinants of the H-2d haplotype, and expanded in tissue culture in the presence of interleukin 2, were capable of rapidly killing lethally irradiated BALB/c recipients when injected intravenously. Mice given 10(6), 10(7) and 10(8) such T cells sur...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:Mercuric chloride (HgCl2)-induced autoimmunity in Brown Norway (BN) rats is a highly polarized polyclonal Th2-driven autoimmune response with increased IgE production, lymphoproliferation, vasculitis and proteinuria. The increase in serum IgE concentration is clearly measurable by day 4 after the first HgCl2 injection...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01928.x
更新日期：2002-09-01 00:00:00
abstract：:Hyperlipidaemia accompanies chronic renal disease either as a consequence of the renal dysfunction or as part of generalized metabolic derangements. Under both situations, the lipid profile is characterized by accumulation of triglyceride-rich lipoproteins (TGRLs). This lipid profile is recognized as a risk factor for...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13111
更新日期：2018-06-01 00:00:00
abstract：:One hundred and sixty-six unrelated children and adolescents having growth-onset, insulin-dependent diabetes mellitus (IDD) were studied for pancreatic-cytoplasmic islet cell antibodies (ICA) at various times from the diagnosis of the disease. A strong association between HLA antigens A9 and Bw16 and an absence of ICA...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:We have produced monoclonal antibodies (MoAb) to the Rb core and lipid A regions of Salmonella lipopolysaccharide (LPS) and have assessed their ability to inhibit LPS-mediated mitogenic responses in vitro, and to protect against LPS toxicity and lethal Salmonella infection in vivo. Monoclonal antibodies RC-8 and RC-16...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Native human CRP in solution formed complexes with the abnormal lipoprotein beta-VLDL in serum of patients with type III hyperlipoproteinaemia. CRP also formed complexes in sera from individuals with type IV and type V hyperlipoproteinaemia. The binding was calcium-dependent and inhibitable by free phosphoryl choline....
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Psoriasis (Ps), psoriatic arthritis (PsA) and rheumatoid arthritis (RA) are common diseases dependent on environmental factors that activate the immune system in unknown ways. Mannan is a group of polysaccharides common in the environment; they are potentially pathogenic, because at least some of them induce Ps-, PsA-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13268
更新日期：2019-06-01 00:00:00
abstract：:The sera of patients with histologically proven carcinomata of the gastrointestinal tract were fractionated by gel filtration and the fractions assayed for the presence of free carcinoembryonic antigen (CEA) binding immunoglobulins and CEA immune complexes by radioimmuno-double-diffusion, using 125I-CEA as a marker. I...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
