Mutagen-induced changes in cellular deoxycytidine triphosphate and thymidine triphosphate in Chinese hamster ovary cells.

abstract：AIM:AF1q has a precise oncogenic function. The purpose of this study is to investigate whether CBD has an effect on the AF1q/ICAM-1 regulatory axis in Burkitt's lymphoma (BL), and thus has potential to enhance immunotherapy and reduce side effects. METHODS:We established BL cell lines with altered AF1q expression usin...

journal_title：Biochemical and biophysical research communications

authors： Togano T,Kim N,Kim N,Park GS,Park AK,Bennet M,Park J

更新日期：2019-11-26 00:00:00

abstract：:Recent reports have indicated that circular RNA (circRNA) may regulate Lung adenocarcinoma (LAC) development. Our previous studies showed that hsa_circ_0012673 was up-regulated in a circRNA microarray. However, its expression level in LAC has not been verified, and the underlying molecular mechanisms in LAC are unknow...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Zhu X,Zhang H,Wei S,Chen Y,Chen Y,Wang F,Fan X,Han S,Wu G

更新日期：2018-02-19 00:00:00

abstract：:We report the half-lives for two proteins involved in the regulation of intracellular calcium in the brain: the plasma membrane Ca-ATPase and its regulatory protein, calmodulin. [14C]-labeled leucine was injected into seven month old adult Fischer 344 rats and the time-dependent appearance and loss of radioactivity wa...

journal_title：Biochemical and biophysical research communications

authors： Ferrington DA,Chen X,Krainev AG,Michaelis EK,Bigelow DJ

更新日期：1997-08-08 00:00:00

abstract：:We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by b...

journal_title：Biochemical and biophysical research communications

authors： Rhee WJ,Kim EJ,Park TH

更新日期：2002-07-26 00:00:00

abstract：:The activity of neutral sphingomyelinase (EC 3.1.4.12) in a plasma membrane enriched fraction was found to be increased in dexamethasone treated cells. The elevation of sphingomyelinase activity was blocked by cycloheximide indicating that protein synthesis was required for the steroid action. Ceramidase (EC3.5.1.23) ...

journal_title：Biochemical and biophysical research communications

authors： Ramachandran CK,Murray DK,Nelson DH

更新日期：1990-03-16 00:00:00

abstract：:5-Bromodeoxyuridine (BrdU) modulates expression of particular genes associated with cellular differentiation and senescence. Our previous studies have suggested an involvement of chromatin structure in this phenomenon. Here, we examined the effect of 5-bromouracil on nucleosome positioning in vivo using TALS plasmid i...

journal_title：Biochemical and biophysical research communications

authors： Miki K,Shimizu M,Fujii M,Hossain MN,Ayusawa D

更新日期：2008-04-11 00:00:00

abstract：:The self-assembly of amyloid-beta (Aβ) proteins in aqueous extracellular environments is implicated in Alzheimer's disease. Among several alloforms of Aβ proteins differing in sequence length, the 42- and 40-residue forms (Aβ42 and Aβ40) are the most abundant ones in the human body. Although the only difference is the...

journal_title：Biochemical and biophysical research communications

authors： Lin Y,Im H,Diem LT,Ham S

更新日期：2019-03-12 00:00:00

abstract：:Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR fol...

journal_title：Biochemical and biophysical research communications

authors： Al-Atrash G,Shetty S,Idell S,Xue Y,Kitson RP,Halady PK,Goldfarb RH

更新日期：2002-03-22 00:00:00

abstract：:An altered metabolism supports growth of tumor. AKT, a major signal integrator plays a key role in cell metabolism. We have shown that nutritional deprivation activates AKT as observed by increased phosphorylation of both Thr308 and Ser473. Pharmacological inhibition or silencing of AKT by siRNA affects cell viability...

journal_title：Biochemical and biophysical research communications

authors： Sudhagar S,Sathya S,Gokulapriya G,Lakshmi BS

更新日期：2016-03-18 00:00:00

abstract：:Helical hairpins, two closely spaced helical membrane spanning segments separated by a short surface turn, are thought to be common in integral membrane proteins. Here, we study the membrane integration of a naturally occurring helical hairpin from the secretory Na(+)-K(+)-2Cl(-) cotransporter NKCC1. This sequence is ...

journal_title：Biochemical and biophysical research communications

authors： Nagy A,Turner RJ

更新日期：2007-05-04 00:00:00

abstract：:Destruxin B, a peptide antibiotic, inhibits vacuolar-type ATPase (V-ATPase) specifically and dose-dependently among ATPases examined. Acidification of intracellular organelles is also blocked by destruxin B at comparable concentrations when assessed by accumulation of acridine orange. The inhibitory activity of destru...

journal_title：Biochemical and biophysical research communications

authors： Muroi M,Shiragami N,Takatsuki A

更新日期：1994-12-15 00:00:00

abstract：:Reaction of the reduced (pink) form of the purple acid phosphatase from beef spleen with excess phosphate at pH 5.0, monitored by optical and low temperature EPR spectroscopy and by measurement of enzymatic activity, results in parallel loss of activity and oxidation of the iron chromophore. Colorimetric and radiochem...

journal_title：Biochemical and biophysical research communications

authors： Burman S,Davis JC,Weber MJ,Averill BA

更新日期：1986-04-29 00:00:00

abstract：:ATP-binding cassette sub-family G member 2 (ABCG2) confers to the major phenotypes of side population (SP) cells, the cancer stem-like cells. In this study, the SP cells displayed a distinctly higher ABCG2 expression level, sphere formation efficiency (SFE) and growth rate even under hypoxia condition. CXCR4 overexpre...

journal_title：Biochemical and biophysical research communications

authors： He C,Zhang H,Wang B,He J,Ge G

更新日期：2018-10-28 00:00:00

abstract：:The loss of paramagnetism of the stable free radical 4,4'-di-tert.-butyl- diphenyl-1-nitroxy during illumination of the photosensitizer meso-tetrahydroxyphenyl chlorine by light above 620 nm in the absence of oxygen has been followed by kinetic ESR spectroscopy. Addition of the spin trap alpha-(4-pyridyl-1-oxide)- N-t...

journal_title：Biochemical and biophysical research communications

authors： Kriska T,Korecz L,Nemes I,Gál D

更新日期：1995-10-04 00:00:00

abstract：:The mammalian-target-of-rapamycin (mTOR) is a multidomain protein that is important in regulating several components of the translational machinery. mTOR signalling is stimulated by hormones (e.g., insulin) and by amino acids. Our recent data suggest that TOR signalling responds to intracellular amino acids rather tha...

journal_title：Biochemical and biophysical research communications

authors： Proud CG

更新日期：2004-01-09 00:00:00

abstract：:The tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL or Apo2L) and its receptors are members of the tumor necrosis factor superfamily. TRAIL triggers apoptosis by binding to its two proapoptotic receptors DR4 and DR5, a process which is negatively regulated by binding of TRAIL to its two decoy rece...

journal_title：Biochemical and biophysical research communications

authors： Sun SY,Yue P,Zhou JY,Wang Y,Choi Kim HR,Lotan R,Wu GS

更新日期：2001-01-26 00:00:00

abstract：:The vasodilator action of organic nitrates is thought to be mediated by an increase in the level of cGMP following stimulation of the cytosolic enzyme guanylate cyclase in the vascular smooth muscle cell. However, direct evidence for the formation of the putative active metabolite, nitric oxide (NO) within the differe...

journal_title：Biochemical and biophysical research communications

authors： Feelisch M,Kelm M

更新日期：1991-10-15 00:00:00

abstract：:Deficiency of Smad3, an intracellular mediator of TGF-β, was shown to significantly accelerate re-epithelialization of the colonic mucosa. This study was performed to investigate the molecular mechanisms by which Smad3 controls colonic epithelial cell proliferation and crypt formation. Smad3(ex8/ex8) C57BL/6 mice were...

journal_title：Biochemical and biophysical research communications

authors： Furukawa K,Sato T,Katsuno T,Nakagawa T,Noguchi Y,Tokumasa A,Yokote K,Yokosuka O,Saito Y

更新日期：2011-02-25 00:00:00

abstract：:Understanding protein aggregation may hold important clues to understanding what goes wrong with protein folding in neurodegenerative disorders and in bioreactors in which proteins are overexpressed. Unfortunately, aggregates tend to be intractable to most standard methods of biochemical investigation. Thus, relativel...

journal_title：Biochemical and biophysical research communications

authors： Kundu B,Guptasarma P

更新日期：2002-04-26 00:00:00

abstract：:GTP gamma S-dependent phospholipase D (PLD) activity time-dependently increased during differentiation of rat C6 glioma cells to astrocytic phenotypes induced by dibutyryl cyclic AMP (dbcAMP)/theophylline. The changes in PLD mRNA level were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method us...

journal_title：Biochemical and biophysical research communications

authors： Yoshimura S,Nakashima S,Ohguchi K,Sakai H,Shinoda J,Sakai N,Nozawa Y

更新日期：1996-08-14 00:00:00

abstract：:We have compared the sequence and gene order of meta-cleavage pathway operons from alpha- and gamma-subgroups of the Proteobacteria with operons from Burkholderia sp. strain RP007 which belongs to the beta-subgroup of the Proteobacteria. Burkholderia RP007 was isolated for its ability to degrade phenanthrene and conta...

journal_title：Biochemical and biophysical research communications

authors： Laurie AD,Lloyd-Jones G

更新日期：1999-08-19 00:00:00

abstract：:In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA synthesis reaction from standard RNA with a primer containing a tag of 14 randomized bases and the RT to be tested, PCR using high-fidelity DNA polym...

journal_title：Biochemical and biophysical research communications

authors： Yasukawa K,Iida K,Okano H,Hidese R,Baba M,Yanagihara I,Kojima K,Takita T,Fujiwara S

更新日期：2017-10-14 00:00:00

abstract：:Negative regulator of differentiation 1 (Nrd1) is known as a negative regulator of sexual differentiation in fission yeast. Recently, it has been revealed that Nrd1 also regulates cytokinesis, in which physical separation of the cell is achieved by a contractile ring comprising many proteins including actin and myosin...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi A,Kanaba T,Satoh R,Fujiwara T,Ito Y,Sugiura R,Mishima M

更新日期：2013-07-19 00:00:00

abstract：:Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play important roles in the olfactory recognition. The orange blossom midge, Sitodiplosis mosellana is a cereal specialist, and utilizes pheromone and host odorant as a cue for its mating and oviposition. Howe...

journal_title：Biochemical and biophysical research communications

authors： Gong ZJ,Miao J,Duan Y,Jiang YL,Li T,Wu YQ

更新日期：2014-02-07 00:00:00

abstract：:Stimulation of DNA synthesis by EGF was compared in cultured periportal and perivenous hepatocyte populations. Periportal hepatocytes responded to EGF more sensitive (IC50-values 20 vs 75 ng/ml) and with a higher maximal stimulation (420 vs 290%) than perivenous hepatocytes with respect to both [3H]thymidine incorpora...

journal_title：Biochemical and biophysical research communications

authors： Gebhardt R,Jonitza D

更新日期：1991-12-31 00:00:00

abstract：:EGF stimulates gene expression through a variety of signal transduction pathways that include the ras-Erk pathway. We have shown previously that EGF receptor activation stimulates gastrin gene expression through a GC-rich element called gERE. This element binds Sp1 family members and raises the possibility that the ra...

journal_title：Biochemical and biophysical research communications

authors： Merchant JL,Du M,Todisco A

更新日期：1999-01-19 00:00:00

abstract：:Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. To understand the possible cellular processes in which Nek2 participates during the cell cycle, we investigated the expression and subcellular localization of Nek2 in mitotic cells. The Nek2 protein...

journal_title：Biochemical and biophysical research communications

authors： Ha Kim Y,Yeol Choi J,Jeong Y,Wolgemuth DJ,Rhee K

更新日期：2002-01-18 00:00:00

abstract：:We describe here two human VIP receptor cDNA clones isolated from a jejunal epithelial cell cDNA library. The hIVR8 cDNA encodes a human VIP receptor consisting of 460 amino acids and has seven putative transmembrane domains like other G protein-coupled receptors. When expressed in COS-7 cells, hIVR8 conferred specifi...

journal_title：Biochemical and biophysical research communications

authors： Couvineau A,Rouyer-Fessard C,Darmoul D,Maoret JJ,Carrero I,Ogier-Denis E,Laburthe M

更新日期：1994-04-29 00:00:00

abstract：:Mutations of the glycogen branching enzyme gene, GBE1, result in glycogen storage disease (GSD) type IV, an autosomal recessive disorder having multiple clinical forms. One mutant allele of this gene, GBE1 c.1076A>C, has been reported in Ashkenazi Jewish cases of an adult-onset form of GSD type IV, adult polyglucosan ...

journal_title：Biochemical and biophysical research communications

authors： Hussain A,Armistead J,Gushulak L,Kruck C,Pind S,Triggs-Raine B,Natowicz MR

更新日期：2012-09-21 00:00:00

abstract：:Human neuropeptide Y (NPY) analog ANA-NPY or [L17, Q19, A20, A23, L28, L31]NPY (13-36)-amide binds weakly to postjunctional receptors to raise blood pressure but binds tightly to prejunctional receptors to inhibit neurotransmitter release. ANA-NPY forms a well-conserved anti-parallel helical structure overlapping E23-...

journal_title：Biochemical and biophysical research communications

authors： Barden JA

更新日期：1995-10-04 00:00:00