The interaction of phosphate with the purple acid phosphatase from beef spleen: evidence that phosphate binding is accompanied by oxidation of the iron chromophore.

abstract：:p66Shc plays a key role in oxidative stress-induced apoptosis. p66Shc gene expression is tissue-specific and controlled by promoter methylation. In T-cells p66Shc expression is induced by a variety of apoptotic stimuli. We have addressed the mechanisms regulating p66Shc expression in T-cells. We show that the increase...

journal_title：Biochemical and biophysical research communications

authors： Pezzicoli A,Ulivieri C,Capitani N,Ventura A,Pelicci P,Baldari CT

更新日期：2006-10-13 00:00:00

abstract：:MicroRNAs (miRNAs) play crucial roles in the pancreatic carcinogenesis and progression. In the present study, we found that miR-9-5p was significantly downregulated in pancreatic cancer tissues and cell lines. The expression levels of miR-9-5p were negatively correlated with tumor stage and vessel invasion. Log-rank t...

journal_title：Biochemical and biophysical research communications

authors： Wang J,Wang B,Ren H,Chen W

更新日期：2019-01-29 00:00:00

abstract：:The observation that spermine inhibits the endotoxin (lipopolysaccharide; LPS) induced production of nitric oxide (NO) in macrophages has been ascribed to the conversion of SP to active metabolites by the action of enzymes, such as diamine oxidases, found, for example, in bovine sera. Inhibitory effect is also observe...

journal_title：Biochemical and biophysical research communications

authors： Southan GJ,Szabó C,Thiemermann C

更新日期：1994-09-30 00:00:00

abstract：:CrkII is an intracellular adaptor protein involved in signal transduction by various growth factors. Activation of PDGF alpha-receptor resulted in its association with CrkII in vivo. In contrast, binding of CrkII to the PDGF beta-receptor was negligible, despite its becoming prominently phosphorylated. Bacterially exp...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto T,Yokote K,Take A,Takemoto M,Asaumi S,Hashimoto Y,Matsuda M,Saito Y,Mori S

更新日期：2000-04-02 00:00:00

abstract：:Our previous study indicates that hyperinsulinemia in metabolic syndrome in the absence of nephropathy may promote hypertension by stimulating renal proximal tubule (PT) sodium transport via insulin receptor substrate (IRS) 2/phosphoinositide 3-kinase pathway. In the present study we showed that the stimulatory effect...

journal_title：Biochemical and biophysical research communications

authors： Nakamura M,Satoh N,Suzuki M,Kume H,Homma Y,Seki G,Horita S

更新日期：2015-05-22 00:00:00

abstract：:In the present study we evaluated the effect of interleukin 1-beta on adenylate cyclase activity in 235-1 pituitary cell line. The dose-response curve of interleukin 1 beta effect on adenylate cyclase activity showed a significant inhibition of basal enzyme activity at 1 pM concentration, while the inhibition of forsk...

journal_title：Biochemical and biophysical research communications

authors： Schettini G,Florio T,Meucci O,Scala G,Landolfi E,Grimaldi M

更新日期：1988-09-30 00:00:00

abstract：:Borate/boric acid solutions have distinctive effects on the absorption and fluorescence emission spectra of dityrosine. In the presence of excess borate/boric acid, the fluorescence emission maximum of the singly ionized dityrosine chromophore shifts from 407 nm (quantum yield = 0.80) to 374 nm (quantum yield = 0.14)....

journal_title：Biochemical and biophysical research communications

authors： Malencik DA,Anderson SR

更新日期：1991-07-15 00:00:00

abstract：:Bovine rhodopsin was recombined with various phospholipids in which the lipid acyl chain composition was held constant at that of egg phosphatidylcholine (PC), while the identity of the headgroups was varied. The ratio of MII/MI produced in the recombinant membrane vesicles by an actinic flash was studied as a functio...

journal_title：Biochemical and biophysical research communications

authors： Gibson NJ,Brown MF

更新日期：1991-04-30 00:00:00

abstract：:Human natural killer enhancing factor genes (NKEF-A and B) share extensive homology with several genes from other organisms known to be induced by oxidative stress and to protect organisms and proteins from oxidative destruction. In this study, we have identified natural killer enhancing factor as an abundant protein ...

journal_title：Biochemical and biophysical research communications

authors： Shau H,Kim A

更新日期：1994-02-28 00:00:00

abstract：:Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglyc...

journal_title：Biochemical and biophysical research communications

authors： Dhand R,Sharma VK,Teng AL,Krishnasamy S,Gross NJ

更新日期：1998-03-27 00:00:00

abstract：:The MoFe protein of the complex metalloenzyme nitrogenase folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits, encoded by the nifD and the nifK genes respectively. Recently, the functional expression of a fusion NifD-K protein of nitrogenase was demonstrated in Azotobacter vi...

journal_title：Biochemical and biophysical research communications

authors： Lahiri S,Pulakat L,Gavini N

更新日期：2005-11-18 00:00:00

abstract：:We have recorded multi-frequency EPR spectra of 63Cu- and 65Cu-labeled, water-soluble CuA-protein from the cytochrome ba3 of T. thermophilus. The spectrum taken at the highest frequency (34.03 GHz) shows no hyperfine structure and is nominally axial with apparent gz approximately 2.18 and gxy approximately 2.00. The s...

journal_title：Biochemical and biophysical research communications

authors： Fee JA,Sanders D,Slutter CE,Doan PE,Aasa R,Karpefors M,Vänngård T

更新日期：1995-07-06 00:00:00

abstract：:It has been suggested that ras proteins are involved in the transmembrane signaling mechanism and they share structural features with GTP-binding proteins. To identify the role of ras oncogene and it's products in the coupling mechanisms of GTP-binding proteins to adenylate cyclase, we examined effect of NaF, cholera ...

journal_title：Biochemical and biophysical research communications

authors： Konishi-Imamura L,Noda M,Nomura Y

更新日期：1987-07-15 00:00:00

abstract：:Profilin, an actin-binding protein, was previously described as a ubiquitous allergen which is responsible for cross-reactivities in about 20% of pollen and food allergic patients. A complete cDNA clone coding for timothy grass (Phelum pratense) pollen profilin was isolated using allergic patients IgE. The deduced ami...

journal_title：Biochemical and biophysical research communications

authors： Valenta R,Ball T,Vrtala S,Duchêne M,Kraft D,Scheiner O

更新日期：1994-02-28 00:00:00

abstract：:The HCMV (human cytomegalovirus) encodes numerous proteins which function to evade the immune response, which allows the virus to replicate. Exploring the mechanisms of HCMV immune escape helps to find the strategy to inhibit HCMV replicate. CD8+ T cells play a critical role in the immune response to viral pathogens. ...

journal_title：Biochemical and biophysical research communications

authors： Hu L,Wen Z,Chen J,Chen Y,Jin L,Shi H,Chen J,Chen J

更新日期：2020-11-12 00:00:00

abstract：:MOTS-c, as a mitochondria derived peptide, exerts benefits for insulin resistance in HFD mice and against various stresses in an AMPK dependent way. Here, in the D-galactose chronic injection models, exogenous MOTS-c was given to determine its direct anti-aging effects. The body weight, insulin sensitivity and blood g...

journal_title：Biochemical and biophysical research communications

authors： Li Q,Lu H,Hu G,Ye Z,Zhai D,Yan Z,Wang L,Xiang A,Lu Z

更新日期：2019-05-28 00:00:00

abstract：:Growing evidence indicates that circular RNA (circRNA) plays an important role in the regulation of tumor biological behaviors. In this study, we aimed to explore the role of a novel circRNA, circ_0034642, in glioma. qRT-PCR was conducted to evaluate the levels of circ_0034642 in glioma tissues and cells. In addition,...

journal_title：Biochemical and biophysical research communications

authors： Yang M,Li G,Fan L,Zhang G,Xu J,Zhang J

更新日期：2019-01-15 00:00:00

abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

journal_title：Biochemical and biophysical research communications

authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

更新日期：2002-02-15 00:00:00

abstract：:The rat hemopexin (Hx) gene was isolated and studies of its transcriptional regulation initiated. For analysis by a transient expression assay, the sequence between -2400 and +21 and sequential 5' truncates were linked to the chloramphenicol acetyltransferase (CAT) gene. HepG2 cells transfected with these CAT construc...

journal_title：Biochemical and biophysical research communications

authors： Nagae Y,Muller-Eberhard U

更新日期：1992-05-29 00:00:00

abstract：:N-myc downstream regulated gene 1 (NDRG1) is an important gene regulating tumor invasion. In this study, shRNA technology was used to suppress NDRG1 expression in CaSki (a cervical cancer cell line) and HO-8910PM (an ovarian cancer cell line). In vitro assays showed that NDRG1 knockdown enhanced tumor cell adhesion, m...

journal_title：Biochemical and biophysical research communications

authors： Zhao G,Chen J,Deng Y,Gao F,Zhu J,Feng Z,Lv X,Zhao Z

更新日期：2011-04-29 00:00:00

abstract：:Activation of seven-transmembrane G-protein coupled receptor (GPCR) mediates extracellular signals into intracellular responses. G-protein coupled receptor 55 (GPR55) is one of GPCRs and activated by endogenous cannabinoids. A family of regulators of G-protein signaling (RGS) stimulates GTP hydrolysis of alpha subunit...

journal_title：Biochemical and biophysical research communications

authors： Jang H,Kim Y,Ghil S

更新日期：2020-12-17 00:00:00

abstract：:The activation of c-jun N-terminal kinase (JNK) in pancreatic islets is associated with impaired function and viability, and JNK inhibitory peptides (JNKIs) are cytoprotective. In particular, l-isoforms of JNKIs were shown to improve islets viability, while the d-retroinverso isoform of JNKI (RI-JNKI), with a higher t...

journal_title：Biochemical and biophysical research communications

authors： Fornoni A,Cobianchi L,Sanabria NY,Pileggi A,Molano RD,Ichii H,Rosero S,Inverardi L,Ricordi C,Pastori RL

更新日期：2007-03-02 00:00:00

abstract：:Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S pha...

journal_title：Biochemical and biophysical research communications

authors： Kim BJ,Kang KM,Jung SY,Choi HK,Seo JH,Chae JH,Cho EJ,Youn HD,Qin J,Kim ST

更新日期：2008-07-25 00:00:00

abstract：:Treatment of colorectal cancer (CRC) remains a challenge because of the lack of effective early treatment strategies and high incidence of relapse. 5-Fluorouracil (5-FU) is a typical CRC treatment. Bromosporine is an innovative bromodomain and extraterminal domain (BET) inhibitor. We investigated if CRC could be targe...

journal_title：Biochemical and biophysical research communications

authors： Cheng X,Huang Z,Long D,Jin W

更新日期：2020-01-22 00:00:00

abstract：:Using a novel method consisting of chromatin fractionation and allele-specific detection, chromatin packaging is compared between active X (Xa) and inactive X (Xi) chromosomes for five tumor cell clones that were derived from inter-subspecific F1 female mice. Separation of heterochromatic (H) and euchromatic (E) fract...

journal_title：Biochemical and biophysical research communications

authors： Endo Y,Watanabe T,Kuwabara K,Tsunashima K,Mishima Y,Arakawa M,Takagi N,Kominami R

更新日期：1998-03-06 00:00:00

abstract：:Conjugated linoleic acid (CLA), a group of minor fatty acids from ruminant origin, has long been recognized as a body fat lowering agent. Given the trans(t)10,cis(c)12-CLA well documented interference on lipolysis, we hypothesized for adipocytes altered permeation to glycerol when supplemented with this isomer. 3T3-L1...

journal_title：Biochemical and biophysical research communications

authors： Martins SV,Madeira A,Lopes PA,Pires VM,Alfaia CM,Prates JA,Moura T,Soveral G

更新日期：2015-03-06 00:00:00

abstract：:X-linked Emery-Dreifuss muscular dystrophy is usually caused by absence of the nuclear membrane protein, emerin, due to nonsense mutations or deletions, but a few missense mutations also exist. A pathogenic g993t mutation causes a Q133H change in the nuclear targeting region of emerin, but it may also reduce emerin le...

journal_title：Biochemical and biophysical research communications

authors： Holt I,Clements L,Manilal S,Morris GE

更新日期：2001-10-12 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may play important roles during hepatitis B virus (HBV) infection. In this study, we used a recombinant human soluble death receptor 5 (sDR5) to explore its effect in a mouse model of HBV-induced acute hepatitis. By measuring blood transaminase activity a...

journal_title：Biochemical and biophysical research communications

authors： Liu YG,Liu SX,Liang XH,Zhang Q,Gao LF,Han LH,Cao YL,Hou N,Du J,Sun WS

更新日期：2007-01-12 00:00:00

abstract：:Lactobacillus, a representative useful bacterium, in the intestinal tract was found to bind to some specific glycosphingolipids, like the pathogenic intestinal bacteria. Thin layer chromatography overlay assays using rabbit antiserum against Lactobacillus casei revealed that the bacteria bound to GA1 and trihexosylcer...

journal_title：Biochemical and biophysical research communications

authors： Yamamoto K,Miwa T,Taniguchi H,Nagano T,Shimamura K,Tanaka T,Kumagai H

更新日期：1996-11-01 00:00:00

abstract：:Store-operated Ca(2+) entry (SOCE) is crucial for various physiological responses in immune cells. Although it is known that STIM1 relocates into discrete puncta juxtaposed to the plasma membrane to initiate SOCE, the machinery modulating the function of STIM1 remains unclear. We explored to find its modulators using ...

journal_title：Biochemical and biophysical research communications

authors： Fujii Y,Shiota M,Ohkawa Y,Baba A,Wanibuchi H,Kinashi T,Kurosaki T,Baba Y

更新日期：2012-06-15 00:00:00