Abstract:
:In this study, we examined the effect of arachidonic acid (AA) on the BK alpha-subunit with or without beta-subunits expressed in Xenopus oocytes. In excised patches, AA potentiated the hSlo-alpha current and slowed inactivation only when beta2/3 subunit was co-expressed. The beta2-subunit-dependent modulation by AA persisted in the presence of either superoxide dismutase or inhibitors of AA metabolism such as nordihydroguaiaretic acid and eicosatetraynoic acid, suggesting that AA acts directly rather than through its metabolites. Other cis unsaturated fatty acids (docosahexaenoic and oleic acid) also enhanced hSlo-alpha + beta2 currents and slowed inactivation, whereas saturated fatty acids (palmitic, stearic, and caprylic acid) were without effect. Pretreatment with trypsin to remove the cytosolic inactivation domain largely occluded AA action. Intracellularly applied free synthetic beta2-ball peptide induced inactivation of the hSlo-alpha current, and AA failed to enhance this current and slow the inactivation. These results suggest that AA removes inactivation by interacting, possibly through conformational changes, with beta2 to prevent the inactivation ball from reaching its receptor. Our data reveal a novel mechanism of beta-subunit-dependent modulation of BK channels by AA. In freshly dissociated mouse neocortical neurons, AA eliminated a transient component of whole cell K(+) currents. BK channel inactivation may be a specific mechanism by which AA and other unsaturated fatty acids influence neuronal death/survival in neuropathological conditions.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Sun X,Zhou D,Zhang P,Moczydlowski EG,Haddad GGdoi
10.1152/jn.00700.2006subject
Has Abstractpub_date
2007-01-01 00:00:00pages
62-9issue
1eissn
0022-3077issn
1522-1598pii
00700.2006journal_volume
97pub_type
杂志文章abstract::1. The actions of hydrogen sulfide (HS-) on membrane and synaptic properties of dorsal raphe (DR) serotonergic cells were studied in the in vitro brain stem slice preparation, using intracellular sharp microelectrode and whole-cell recording techniques. 2. Sulfide produced two reversible, concentration-dependent effec...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.1.81
更新日期:1993-07-01 00:00:00
abstract::Acetylcholine increases intracellular calcium of arterial chemoreceptor cells of adult cats. J. Neurophysiol. 78: 2388-2395, 1997. Several neurotransmitters have been reported to play important roles in the chemoreception of the carotid body. Among them acetylcholine (ACh) appears to be involved in excitatory processe...
journal_title:Journal of neurophysiology
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更新日期:1997-11-01 00:00:00
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journal_title:Journal of neurophysiology
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更新日期:2012-08-01 00:00:00
abstract::The contribution of ascending afferents to the control of speech movement was evaluated by applying unanticipated loads to the lower lip during the generation of combined upper lip-lower lip speech gestures. To eliminate potential contamination due to anticipation or adaptation, loads were applied randomly on only 10-...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.51.4.705
更新日期:1984-04-01 00:00:00
abstract::The purpose of this study was to examine which primary afferent fibers are sensitized to mechanical stimuli after an experimental surgical incision to the glabrous skin of the rat hindpaw. Afferent fibers teased from the L(5) dorsal root or the tibial nerve were recorded in anesthetized rats. The mechanical response p...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00207.2001
更新日期:2002-02-01 00:00:00
abstract::1. Properties of nucleus incertus (NIC) neurons projecting to the cerebellar flocculus were studied in alert cats by using chronic unit and eye movement recording and antidromic activation. Projection of these neurons onto the flocculus was verified with retrograde transport of horseradish peroxidase after injections ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.3.1367
更新日期:1995-09-01 00:00:00
abstract::The aim of this study was to determine whether neuronal activity in the macaque supplementary eye field (SEF) is influenced by the rule used for saccadic target selection. Two monkeys were trained to perform a variant of the memory-guided saccade task in which any of four visible dots (rightward, upward, leftward, and...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.3.1369
更新日期:2000-09-01 00:00:00
abstract::The lateral superior olive (LSO) is the primary auditory nucleus for processing of interaural sound level differences, which is one of the major cues for sound localization. During development, survival and maturation of LSO neurons critically depend on synaptic activity and intracellular calcium signaling. Before hea...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00686.2006
更新日期:2007-05-01 00:00:00
abstract::1. The purpose of this study was to use the techniques of spike- and stimulus-triggered averaging (SpTA and StTA, respectively) to examine the output organization of individual rubromotoneuronal (RM) cells in relation to clusters of neighboring cells. SpTA of electromyographic (EMG) activity in awake monkeys reveals t...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.66.6.1978
更新日期:1991-12-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1152/jn.1997.77.3.1325
更新日期:1997-03-01 00:00:00
abstract::1. Spontaneous saccades, vestibuloocular responses (VOR), and optokinetic nystagmus were recorded in three squirrel monkeys before and after chemical or electrolytic lesion of the posterior commissure (PC). 2. PC lesions produced abnormal vertical eye movements, in particular, 1) Postsaccadic drifts, and 2) VOR gain r...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.6.2582
更新日期:1994-06-01 00:00:00
abstract::The activity of malic dehydrogenase, pyruvic kinase, and phosphorylase b was measured in the geniohyoid muscle of the cat after injection of 10 10 mM colchicine into the hypoglossal nerve. Experiments performed 1-60 days after the injection showed that the activity of the three enzymes gradually decreased (day 4-5), r...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.6.1236
更新日期:1976-11-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01107.2006
更新日期:2007-08-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01098.2002
更新日期:2003-05-01 00:00:00
abstract::We have characterized selectivity and sparseness in anterior inferotemporal cortex, using a large data set. Responses were collected from 674 monkey inferotemporal cells, each stimulated by 806 object photographs. This 806 × 674 matrix was examined in two ways: columnwise, looking at responses of a single neuron to al...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00990.2010
更新日期:2011-09-01 00:00:00
abstract::The medial-olivocochlear (MOC) acoustic reflex is thought to provide frequency-specific feedback that adjusts the gain of cochlear amplification, but little is known about how frequency specific the reflex actually is. We measured human MOC tuning through changes in stimulus frequency otoacoustic emissions (SFOAEs) fr...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00549.2011
更新日期:2012-03-01 00:00:00
abstract::Electrically excitable cells have voltage-dependent ion channels on the plasma membrane that regulate membrane permeability to specific ions. Voltage-gated Ca(2+) channels (VGCCs) are especially important as Ca(2+) serves as both a charge carrier and second messenger. Zebrafish (Danio rerio) are an important model ver...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Journal of neurophysiology
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更新日期:2016-05-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of neurophysiology
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更新日期:2000-11-01 00:00:00
abstract::Alzheimer's disease (AD) is a progressive neurodegenerative condition characterized by profound deficits in memory and cognitive function. Neuropathological hallmarks of the disease include a loss of basal forebrain cholinergic neurons and the deposition of beta-amyloid peptide (Abeta) in neuritic plaques. At a cellul...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00616.2003
更新日期:2003-11-01 00:00:00
abstract::Group I metabotropic glutamate receptor (mGluR) agonists increase the excitability of hippocampal CAl pyramidal neurons via depression of the postspike afterhyperpolarization. In adult rats, this is mediated by both mGluR1 and -5, but the signal transduction processes involved are unknown. In this study, we investigat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01236.2003
更新日期:2004-11-01 00:00:00
abstract::1. Plasticity at the connections between sensory neurons and their follower cells in Aplysia has been used extensively as a model system to examine mechanisms of simple forms of learning. Earlier studies have concluded that serotonin (5-HT) is a key modulatory transmitter and that it exerts its short-term actions via ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1992.68.2.643
更新日期:1992-08-01 00:00:00
abstract::The progenitor cells from the anterior part of the neonatal subventricular zone, the SVZa, are unusual in that, although they undergo division, they have a neuronal phenotype. To characterize the electrophysiological properties of the SVZa precursor cells, recordings were made of potassium and sodium currents from SVZ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.1.95
更新日期:1999-01-01 00:00:00
abstract::Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred micrometers below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing ext...
journal_title:Journal of neurophysiology
pub_type:
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更新日期:2004-04-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
abstract::The hypothesis that gamma-band oscillations are related to the representation of an environmental scene in the cerebral cortex after binding of corresponding perceptual elements is currently under discussion. One question is how the sensory system reacts to a fast change in the scene if perceptual elements are rigidly...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
abstract::Spatial and temporal properties of LGN receptive fields were studied by flashing a small bar of light across the field in 28 discrete steps. The flashes at each of the spatial positions were used to produce 28 PST histograms. These histograms were in turn displayed as a plane, with space on the chi axis, time on the p...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.2.213
更新日期:1976-03-01 00:00:00