Metabotropic glutamate receptors in the lateral superior olive activate TRP-like channels: age- and experience-dependent regulation.

Abstract:

:The lateral superior olive (LSO) is the primary auditory nucleus for processing of interaural sound level differences, which is one of the major cues for sound localization. During development, survival and maturation of LSO neurons critically depend on synaptic activity and intracellular calcium signaling. Before hearing onset, glutamatergic synaptic inputs from the cochlear nucleus (CN) to the LSO activate group I metabotropic glutamate receptors (mGluRs), which leads to calcium release from intracellular stores and large calcium influx from the extracellular milieu. Here, we investigated the nature of the mGluR-activated membrane channel that mediates the influx of extracellular calcium. Using Fura-2 calcium imaging in brain stem slices of neonatal and juvenile mice, we found that this calcium channel is blocked by Ni(2+), La(3+), and 2-aminoethoxydiphenylborane (2-APB), known antagonists of transient receptor potential (TRP) channels. During postnatal development, the contribution of extracellular calcium influx to mGluR-mediated Ca(2+) responses gradually decreased and was almost abolished by the end of the third postnatal week. Over this period, the contribution of Ca(2+) release from internal stores remained unchanged. The developmental decrease of TRP-like channel-mediated calcium influx was significantly less in congenitally deaf waltzer mice, suggesting that early auditory experience is necessary for the normal age-dependent downregulation of functional TRP channels.

journal_name

J Neurophysiol

authors

Ene FA,Kalmbach A,Kandler K

doi

10.1152/jn.00686.2006

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

3365-75

issue

5

eissn

0022-3077

issn

1522-1598

pii

00686.2006

journal_volume

97

pub_type

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