Abstract:
:1. The actions of hydrogen sulfide (HS-) on membrane and synaptic properties of dorsal raphe (DR) serotonergic cells were studied in the in vitro brain stem slice preparation, using intracellular sharp microelectrode and whole-cell recording techniques. 2. Sulfide produced two reversible, concentration-dependent effects on resting membrane properties of DR cells: (1) 14% responded to HS- with a slow onset hyperpolarization or an outward current accompanied by an conductance increase in voltage clamp (holding potential = -60 mV; monophasic outward cell) or (2) 39% responded with a rapid-onset depolarization corresponding to a weakly voltage-dependent inward current showing little or no change in conductance between -115 and -40 mV (monophasic inward cell). In addition, 29.5% showed both the above effects, responding first with a rapid-onset depolarization and then a sustained hyperpolarization. Such cells had membrane currents very similar to those seen in the monophasic inward and outward cells (biphasic cells). Finally, 17.5% of DR cells had no measurable postsynaptic membrane response to HS-. 3. The outward current induced in the presence of HS- had a reversal potential of about -90 mV when recorded either with 2 M KCl or 145 mM potassium gluconate in the pipette and was accompanied by an increase in conductance, suggesting that it is caused by an elevated conductance to K+. 4. This current was sensitive to the removal of external Ca2+ and blockade by Cd2+, suggesting that it is activated by an elevation in internal [Ca2+]. It was also blocked by apamin or Ba2+ and Cs+, both of which revealed an underlying inward current. The outward current was insensitive to the application of a large variety of antagonists to other known voltage- and calcium-dependent K+ channels. Elevation of intracellular ATP using a patch pipette did not prevent the activation of the outward current. 5. HS- reversibly suppressed a voltage-dependent outward current activated in the voltage range of -50 to -40 mV. This current was also blocked by 10 mM tetraethylammonium, suggesting that HS- suppresses the delayed rectifier in DR cells. 6. The inward current could be observed in the presence of HS- not only in monophasic inward cells but also in monophasic outward or biphasic cells whose outward current was selectively blocked. This inward current was sensitive to the removal of extracellular Ca2+, or the the application of relatively low concentrations of Cd2+, suggesting that it is carried by Ca2+. Both these manipulations also blocked the outward current in monophasic outward or biphasic cells.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Kombian SB,Reiffenstein RJ,Colmers WFdoi
10.1152/jn.1993.70.1.81subject
Has Abstractpub_date
1993-07-01 00:00:00pages
81-96issue
1eissn
0022-3077issn
1522-1598journal_volume
70pub_type
杂志文章abstract::1. The modulation of reflex responses in up to 10 simultaneously recorded hindlimb muscles was studied in unrestrained cats walking on a treadmill. Single electrical shocks of various strengths were applied to different skin areas of teh hindlimb at different times of the step cycle while the resulting EMG responses w...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1980.44.5.1024
更新日期:1980-11-01 00:00:00
abstract::Seminal work in animals indicates that learning a motor task results in long-term potentiation (LTP) in primary motor cortex (M1) and a subsequent occlusion of LTP induction (Rioult-Pedotti et al. J Neurophysiol 98: 3688-3695, 2007). Using various forms of noninvasive brain stimulation in conjunction with a motor lear...
journal_title:Journal of neurophysiology
pub_type: 评论,杂志文章
doi:10.1152/jn.00766.2013
更新日期:2014-03-01 00:00:00
abstract::Two forms of undulatory locomotion in the lamprey (a lower vertebrate) have been described earlier: fast forward swimming (FFS) used for long distance migrations and slow backward swimming (SBS) used for escape from adverse tactile stimuli. In the present study, we describe another form of escape behavior: slow forwar...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00903.2007
更新日期:2008-01-01 00:00:00
abstract::Identifying the properties of correlations in the firing of neocortical neurons is central to our understanding of cortical information processing. It has been generally assumed, by virtue of the columnar organization of the neocortex, that the firing of neurons residing in a certain vertical domain is highly correlat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00981.2014
更新日期:2015-05-01 00:00:00
abstract::Searching for items that are useful given current goals, or "target" recognition, requires observers to flexibly attend to certain object properties at the expense of others. This could involve focusing on the identity of an object while ignoring identity-preserving transformations such as changes in viewpoint or focu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00015.2019
更新日期:2019-04-01 00:00:00
abstract::A desirable goal of functional MRI (fMRI), both clinically and for basic research, is to produce detailed maps of cortical function in individual subjects. Single-subject mapping of the somatotopic hand representation in the human primary somatosensory cortex (S1) has been performed using both phase-encoding and block...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00499.2012
更新日期:2013-05-01 00:00:00
abstract::The generation of a behaviorally relevant cue to the speed of objects around us is critical to our ability to navigate safely within our environment. However, our perception of speed is often distorted by prevailing conditions. For instance, as luminance is reduced, our perception of the speed of fast-moving patterns ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00359.2012
更新日期:2013-08-01 00:00:00
abstract::1. Serotonergic modulation of a hyperpolarization-activated inward current, Ih, and a calcium-dependent outward current, Io(Ca), was examined in the dorsal gastric (DG) motor neuron, with the use of intracellular recording techniques in an isolated preparation of the crab stomatogastric ganglion (STG). 2. Hyperpolariz...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1992.68.2.496
更新日期:1992-08-01 00:00:00
abstract::The objectives of this study were to probe the contribution of spinal neuron persistent sodium conductances to reflex hyperexcitability in human chronic spinal cord injury. The intrinsic excitability of spinal neurons provides a novel target for medical intervention. Studies in animal models have shown that persistent...
journal_title:Journal of neurophysiology
pub_type: 杂志文章,随机对照试验
doi:10.1152/jn.00570.2010
更新日期:2011-06-01 00:00:00
abstract::Early visual motion signals are local and one-dimensional (1-D). For specification of global two-dimensional (2-D) motion vectors, the visual system should appropriately integrate these signals across orientation and space. Previous neurophysiological studies have suggested that this integration process consists of tw...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00821.2011
更新日期:2012-06-01 00:00:00
abstract::Long-term depression (LTD) of synaptic transmission can be induced by several mechanisms, one thought to involve Ca2+-dependent activation of postsynaptic nitric oxide (NO) synthase and subsequent diffusion of NO to the presynaptic terminal. We used the stable NO donor S-nitroso-N-acetylpenicillamine (SNAP) to study t...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.82.3.1569
更新日期:1999-09-01 00:00:00
abstract::Homosynaptic long-term depression (LTD) of synaptic efficacy was induced in field excitatory postsynaptic potentials by administration of 900 pulses at either 1 or 3 Hz in 2- to 3-wk-old Sprague-Dawley rats. The stimulation was administered via a bipolar stimulating electrode placed immediately adjacent to the recordi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.3.1651
更新日期:1997-03-01 00:00:00
abstract::Discrete anatomic structures in the monkey somatic sensory thalamus may segregate input arising from different peripheral receptors and from different parts of the body. It has been proposed that these structures serve as components of modality- and place-specific pathways from the periphery to the cortex. We now test...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章
doi:10.1152/jn.00756.2005
更新日期:2006-02-01 00:00:00
abstract::Previous behavioral work has shown the existence of both anticipatory and reactive grip force responses to predictable load perturbations, but how the brain implements anticipatory control remains unclear. Here we recorded electroencephalographs while participants were subjected to predictable and unpredictable extern...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01112.2006
更新日期:2008-02-01 00:00:00
abstract::1. To better characterize temporal and spatial mechanisms involved in the coding of prolonged nociceptive stimuli in the spinal cord, the responses of dorsal horn wide dynamic range (WDR) and nociceptive-specific (NS) neurons to prolonged, repetitive noxious heat stimuli (45-49 degrees C) were examined in unanesthetiz...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.69.3.703
更新日期:1993-03-01 00:00:00
abstract::1. Hair cells in whole-mount in vitro preparations of the utricular macula of the bullfrog (Rana catesbeiana) were selected according to their macular location and hair bundle morphology. The voltage responses of selected hair cells to intracellular current steps and sinusoids in the frequency range of 0.5-200 Hz were...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.2.666
更新日期:1994-02-01 00:00:00
abstract::1. Whether complex (C) cells are the only truly texture-sensitive units in the cat's primary visual cortex remains controversial. In view of the strong physiological significance of having putatively only one class of cells sensitive to visual noise in the striate cortex, we reinvestigated this issue. Sensitivities of...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.3.1271
更新日期:1995-09-01 00:00:00
abstract::Converging inputs to the entorhinal cortex from the piriform cortex and medial septum: facilitation and current source density analysis. J. Neurophysiol. 78: 2602-2615, 1997. The entorhinal cortex receives sensory inputs from the piriform cortex and modulatory inputs from the medial septum. To examine short-term synap...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.5.2602
更新日期:1997-11-01 00:00:00
abstract::1. Spontaneous synaptic activity in networks formed by dissociated neurons from embryonic rat midbrain was analyzed in tight seal whole cell recordings. 2. Application of furosemide (0.5 mM) to the cell and its surrounding area increased the frequency of spontaneous synaptic currents. Incubation of the culture with fu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.6.2654
更新日期:1996-06-01 00:00:00
abstract::Learning theories of drug addiction propose that the disorder is, at least in part, attributable to drug effects on accumbal mechanisms that are normally involved in reward-related learning. The neurophysiological mechanisms that might transduce such a drug effect on accumbal mechanisms have yet to be identified. Prev...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00849.2002
更新日期:2003-08-01 00:00:00
abstract::Studies on animals have shown that noise-induced hearing loss is followed by an increase of spontaneous firing at several stages of the central auditory system. This central hyperactivity has been suggested to underpin the perception of tinnitus. It was shown that decreasing cochlear activity can abolish the noise-ind...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00580.2014
更新日期:2015-01-01 00:00:00
abstract::During early development neuronal networks express slow oscillating synchronized activity. The activity can be driven by several, not necessarily mutually exclusive, mechanisms. Each mechanism might have distinctive consequences for the phenomenology, formation, or sustainment of the early activity pattern. Here we st...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00889.2010
更新日期:2011-09-01 00:00:00
abstract::We have characterized selectivity and sparseness in anterior inferotemporal cortex, using a large data set. Responses were collected from 674 monkey inferotemporal cells, each stimulated by 806 object photographs. This 806 × 674 matrix was examined in two ways: columnwise, looking at responses of a single neuron to al...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00990.2010
更新日期:2011-09-01 00:00:00
abstract::After a cortical lesion, cortical areas distant from the site of injury are known to undergo physiological and anatomical changes. However, the mechanisms through which reorganization of distant cortical areas is initiated are poorly understood. In a previous publication, we showed that the ventral premotor cortex (PM...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00792.2006
更新日期:2006-12-01 00:00:00
abstract::Numerous models of the oculomotor neuronal integrator located in the prepositus hypoglossi nucleus (PHN) involve both highly tuned recurrent networks and intrinsic neuronal properties; however, there is little experimental evidence for the relative role of these two mechanisms. The experiments reported here show that ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01355.2005
更新日期:2006-07-01 00:00:00
abstract::Microelectrode studies in monkeys performing short-term memory tasks show the sustained elevated discharge of cortical neurons during the retention of recalled sensory information. Cortical cells that are part of memory networks are assumed to receive numerous inputs of excitatory as well as inhibitory nature and loca...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.4.2219
更新日期:1997-04-01 00:00:00
abstract::1. The synaptic pathways underlying tectal influence over pinna movements were studied using an acute electrophysiological approach. Under pentobarbital anesthesia, postsynaptic potentials were recorded intracellularly in antidromically identified, cat facial motoneurons following electrical stimulation of the superio...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.60.2.769
更新日期:1988-08-01 00:00:00
abstract::We used the framework of the uncontrolled manifold (UCM) hypothesis to explore changes in the structure of variability in multifinger force-production tasks when a secondary task was introduced. Healthy young subjects produced several levels of the total force by pressing with the four fingers of the hand on force sen...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01029.2007
更新日期:2008-02-01 00:00:00
abstract::Neural oscillations are observed ubiquitously in the mammalian brain, but their stability is known to be rather variable. Some oscillations are tonic and last for seconds or even minutes. Other oscillations appear as unstable bursts. Likewise, some oscillations rely on excitatory AMPAergic synapses, but others are GAB...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00119.2020
更新日期:2020-07-01 00:00:00
abstract::1. Excitatory synaptic transmission in mono- and polysynaptic pathways between dorsal root afferents and alpha-motoneurons was studied in the spinal cord preparation of the neonatal rat isolated in vitro, using sharp-electrode intracellular recordings. 2. The duration of monosynaptic excitatory postsynaptic potentials...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.1.406
更新日期:1993-07-01 00:00:00