Functional properties of ryanodine receptors in hippocampal neurons change during early differentiation in culture.

Abstract:

:6-((4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)amino)hexanoic acid ryanodine (BODIPY-ryanodine) binding and Ca(2+) imaging were used to study the properties of ryanodine receptors (RyRs) and cytoplasmic Ca(2+) (Ca) changes in neurons cultured from the embryonic rat hippocampus during the earliest stages of differentiation. Baseline Ca levels declined from 164 +/- 5 (SD) nM at early stages to 70 +/- 4 nM in differentiated neurons. Fluorescent BODIPY-ryanodine binding signals identified activated RyRs in somata, which were eliminated by removal of external Ca(2+) or by blockage of Ca(2+) entry through L-type but not N-type Ca(2+) channels. The GABA synthesis inhibitor 3-mercaptopropionic acid completely abolished ryanodine binding. Caffeine or K(+)-depolarization inhibited the activity of RyRs at very early stages of differentiation but had stimulatory effects at later stages after a network of processes had formed. BayK-8644 stimulated RyRs throughout all regions of all differentiating cells. The results suggest that in differentiating embryonic hippocampal neurons the activity of RyRs is maintained via Ca(2+) entering through L-type Ca(2+) channels. The mode of activation of L-type voltage-gated Ca(2+) channels with either membrane depolarization or specific pharmacological agents affects the coupled activity of RyRs differently as neurons differentiate processes and networks.

journal_name

J Neurophysiol

authors

Sukhareva M,Smith SV,Maric D,Barker JL

doi

10.1152/jn.2002.88.3.1077

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

1077-87

issue

3

eissn

0022-3077

issn

1522-1598

journal_volume

88

pub_type

杂志文章
  • Acutely isolated neurons of the rat globus pallidus exhibit four types of high-voltage-activated Ca2+ current.

    abstract::1. Large, projection-like neurons from the adult (> 3 wk post-natal) rat globus pallidus (GP) were acutely isolated and subjected to whole-cell voltage-clamp (n = 37). Ca2+ currents were isolated pharmacologically in cells with whole-cell capacitances of 15-34 pF. 2. With 5 mM Ba2+ as a charge carrier, whole-cell curr...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.71.3.1272

    authors: Surmeier DJ,Seno N,Kitai ST

    更新日期:1994-03-01 00:00:00

  • Functional properties of neurons in the primate tongue primary motor cortex during swallowing.

    abstract::Recent studies conducted in our laboratory have suggested that the tongue primary motor cortex (i.e., tongue-MI) plays a critical role in the control of voluntary tongue movements in the primate. However, the possible involvement of tongue-MI in semiautomatic tongue movements, such as those in swallowing, remains unkn...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.3.1516

    authors: Martin RE,Murray GM,Kemppainen P,Masuda Y,Sessle BJ

    更新日期:1997-09-01 00:00:00

  • Functional regulation of T-type calcium channels by s-nitrosothiols in the rat thalamus.

    abstract::Although T-type Ca(2+) channels in the reticular thalamic nucleus (nRT) have a central function in tuning neuronal excitability and are implicated in sensory processing, sleep, and epilepsy, the mechanisms involved in their regulation are poorly understood. Here we recorded T-type Ca(2+) currents from intact nRT neuro...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00926.2006

    authors: Joksovic PM,Doctor A,Gaston B,Todorovic SM

    更新日期:2007-04-01 00:00:00

  • Oxygen and seizure dynamics: II. Computational modeling.

    abstract::Electrophysiological recordings show intense neuronal firing during epileptic seizures leading to enhanced energy consumption. However, the relationship between oxygen metabolism and seizure patterns has not been well studied. Recent studies have developed fast and quantitative techniques to measure oxygen microdomain...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00541.2013

    authors: Wei Y,Ullah G,Ingram J,Schiff SJ

    更新日期:2014-07-15 00:00:00

  • EEG generator--a model of potentials in a volume conductor.

    abstract::EEG generator-a model of potentials in a volume conductor. The potential recorded over the cortex electro-corticogram (ECoG) or over the scalp [electroencephalograph (EEG)] derives from the activity of many sources known as "EEG generators." The recorded amplitude is basically a function of the unitary potential of a ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.91143.2008

    authors: Avitan L,Teicher M,Abeles M

    更新日期:2009-11-01 00:00:00

  • Synchronous activity in cat visual cortex encodes collinear and cocircular contours.

    abstract::We explored how contour information in primary visual cortex might be embedded in the simultaneous activity of multiple cells recorded with a 100-electrode array. Synchronous activity in cat visual cortex was more selective and predictable in discriminating between drifting grating and concentric ring stimuli than cha...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01070.2005

    authors: Samonds JM,Zhou Z,Bernard MR,Bonds AB

    更新日期:2006-04-01 00:00:00

  • Cutaneous masking. II. Geometry of excitatory andinhibitory receptive fields of single units in somatosensory cortex of the cat.

    abstract::1. The responses of single neurons in the primary somatosensory cortex of the cat to brief air-pulse stimuli were quantitatively examined. These controlled natural stimuli activated almost exclusively rapidly adapting hair units which, on systematic movement of the stimulus through the receptive field, gave unit-respo...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1979.42.4.1061

    authors: Laskin SE,Spencer WA

    更新日期:1979-07-01 00:00:00

  • Intracortical circuits in rat anterior cingulate cortex are activated by nociceptive inputs mediated by medial thalamus.

    abstract::We investigated the afferents and intracortical synaptic organization of the anterior cingulate cortex (ACC) during noxious electrical stimulation. Extracellular field potentials were recorded simultaneously from 16 electrodes spanning all layers of the ACC in male Sprague-Dawley rats anesthetized by halothane inhalat...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00623.2006

    authors: Yang JW,Shih HC,Shyu BC

    更新日期:2006-12-01 00:00:00

  • Functional characteristics of spontaneously active neurons in rat dorsal cochlear nucleus in vitro.

    abstract::1. The cochlear nucleus of rat brain stem slices was explored with extracellular microelectrodes to determine the distribution and characteristics of spontaneously active neurons. 2. In mapping experiments few spontaneously active neurons were found in anteroventral or posteroventral divisions of the cochlear nucleus....

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.71.2.467

    authors: Waller HJ,Godfrey DA

    更新日期:1994-02-01 00:00:00

  • Pharmacological characterization of extracellular pH transients evoked by selective synaptic and exogenous activation of AMPA, NMDA, and GABAA receptors in the rat hippocampal slice.

    abstract::1. Inhibitors of extracellular carbonic anhydrase (CAo) offer much promise as diagnostic tools in the study of the synaptic basis of activity-induced alkaline transients in the brain. However, most of the present information related to the effects of CAo blockers in nervous tissue comes from experiments that involve s...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.2.633

    authors: Voipio J,Paalasmaa P,Taira T,Kaila K

    更新日期:1995-08-01 00:00:00

  • Mechanisms within the spinal cord are involved in the movement-induced attenuation of an H reflex in the dog.

    abstract::1. H reflexes were elicited in the second interosseous muscle of the hindpaw of the anesthetized dog during passive rotation of the shank about the ipsilateral or contralateral knee. Reflexes sampled at four points in the cycle of movement were compared with stationary controls. For both the ipsilateral and contralate...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.76.5.3589

    authors: Misiaszek JE,Barclay JK,Brooke JD

    更新日期:1996-11-01 00:00:00

  • Phase-locked responses to pure tones in the auditory thalamus.

    abstract::Accurate temporal coding of low-frequency tones by spikes that are locked to a particular phase of the sine wave (phase-locking), occurs among certain groups of neurons at various processing levels in the brain. Phase-locked responses have previously been studied in the inferior colliculus and neocortex of the guinea ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00697.2007

    authors: Wallace MN,Anderson LA,Palmer AR

    更新日期:2007-10-01 00:00:00

  • Early postnatal switch in GABAA receptor α-subunits in the reticular thalamic nucleus.

    abstract::The GABAergic neurons of the thalamic reticular nucleus (nRt) provide the primary source of inhibition within the thalamus. Using physiology, pharmacology, and immunohistochemistry in mice, we characterized postsynaptic developmental changes in these inhibitory projection neurons. First, at postnatal days 3-5 (P3-5), ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00905.2015

    authors: Pangratz-Fuehrer S,Sieghart W,Rudolph U,Parada I,Huguenard JR

    更新日期:2016-03-01 00:00:00

  • Electrotonic architecture of cat gamma motoneurons.

    abstract::1. Experimental measures of input resistance, RN, and responses to brief hyperpolarizing current pulses were obtained in identified gamma-motoneurons in pentobarbital-anesthetized cats using conventional sharp micropipettes. The same cells were subsequently injected with horseradish peroxidase and completely reconstru...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.72.5.2302

    authors: Burke RE,Fyffe RE,Moschovakis AK

    更新日期:1994-11-01 00:00:00

  • Functional NMDA receptors are expressed by both AII and A17 amacrine cells in the rod pathway of the mammalian retina.

    abstract::At many glutamatergic synapses, non-N-methyl-d-aspartate (NMDA) and NMDA receptors are coexpressed postsynaptically. In the mammalian retina, glutamatergic rod bipolar cells are presynaptic to two rod amacrine cells (AII and A17) that constitute dyad postsynaptic partners opposite each presynaptic active zone. Whereas...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00947.2015

    authors: Zhou Y,Tencerová B,Hartveit E,Veruki ML

    更新日期:2016-01-01 00:00:00

  • Temporal limitations in object processing across the human ventral visual pathway.

    abstract::Behavioral studies have shown that object recognition becomes severely impaired at fast presentation rates, indicating a limitation in temporal processing capacity. Here, we studied whether this behavioral limit in object recognition reflects limitations in the temporal processing capacity of early visual areas tuned ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00568.2006

    authors: McKeeff TJ,Remus DA,Tong F

    更新日期:2007-07-01 00:00:00

  • Synaptic potentials evoked by convergent somatosensory and corticocortical inputs in raccoon somatosensory cortex: substrates for plasticity.

    abstract::1. "Unmasking" of weak synaptic connections has been suggested as a mechanism for the early changes in cortical topographic maps that follow alterations of sensory activity. For such a mechanism to operate, convergent sensory inputs must already exist in the normal cortex. 2. We tested for topographic and cross-modali...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1991.66.3.688

    authors: Smits E,Gordon DC,Witte S,Rasmusson DD,Zarzecki P

    更新日期:1991-09-01 00:00:00

  • Combination of neuronal signals representing object-centered location and saccade direction in macaque supplementary eye field.

    abstract::Neurons in the macaque supplementary eye field (SEF) fire at different rates in conjunction with planning saccades in different directions. They also exhibit object-centered spatial selectivity, firing at different rates when the target of the saccade is at the left or right end of a horizontal bar. To compare the rat...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00061.2007

    authors: Moorman DE,Olson CR

    更新日期:2007-05-01 00:00:00

  • Electrophysiology of glutamate neurotoxicity in vitro: induction of a calcium-dependent extended neuronal depolarization.

    abstract::1. Physiological responses of hippocampal pyramidal neurons in primary culture to prolonged glutamate (GLU) exposure (500 microM in all experiments) were studied with the use of patch electrodes and whole-cell current-clamp recording techniques. In some experiments, perforated patch recordings were employed with elect...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.2.362

    authors: Coulter DA,Sombati S,DeLorenzo RJ

    更新日期:1992-08-01 00:00:00

  • Local superfusion modifies the inward rectifying potassium conductance of isolated retinal horizontal cells.

    abstract::1. Horizontal cells were enzymatically and mechanically dissociated from the white perch (Roccus americana) retina and voltage clamped using patch electrodes. Steady-state current-voltage (I-V) relationships of solitary horizontal cells were determined by changing the membrane potential in a rampwise fashion. 2. The I...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1988.60.4.1322

    authors: Perlman I,Knapp AG,Dowling JE

    更新日期:1988-10-01 00:00:00

  • Head-unrestrained gaze adaptation in the rhesus macaque.

    abstract::The ability to adjust the amplitude of gaze shifts in response to persistent visual errors ("gaze adaptation") has been investigated primarily by introducing visual errors at the end of saccades produced by head-restrained primates. Very little is known about the behavior and neural mechanisms underlying gaze adaptati...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90735.2008

    authors: Cecala AL,Freedman EG

    更新日期:2009-01-01 00:00:00

  • A rose by any other name: would it smell as sweet?

    abstract::We examined whether presenting an odor with a positive, neutral, or negative name would influence how people perceive it. In experiment 1, 40 participants rated 15 odors for their pleasantness, intensity, and arousal. In experiment 2, 30 participants passively smelled 10 odors while their skin conductance (SC), heart ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00896.2007

    authors: Djordjevic J,Lundstrom JN,Clément F,Boyle JA,Pouliot S,Jones-Gotman M

    更新日期:2008-01-01 00:00:00

  • Approaching threatening stimuli cause an expansion of defensive peripersonal space.

    abstract::When sudden environmental stimuli signaling threat occur in the portion of space surrounding the body (defensive peripersonal space), defensive responses are enhanced. Recently Bisio et al. (Bisio A, Garbarini F, Biggio M, Fossataro C, Ruggeri P, Bove M. J Neurosci 37: 2415-2424, 2017) showed that a marker of defensiv...

    journal_title:Journal of neurophysiology

    pub_type: 评论,杂志文章

    doi:10.1152/jn.00316.2017

    authors: Bufacchi RJ

    更新日期:2017-10-01 00:00:00

  • Reaching movements are automatically redirected to nearby options during target split.

    abstract::Motor behavior often occurs in environments with multiple goal options that can vary during the ongoing action. We explored this situation by requiring subjects to select between different target options during an ongoing reach. During split trials the original target was replaced with a left and a right flanking targ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00336.2020

    authors: Kurtzer IL,Muraoka T,Singh T,Prasad M,Chauhan R,Adhami E

    更新日期:2020-10-01 00:00:00

  • Monaural and binaural spectrum level cues in the ferret: acoustics and the neural representation of auditory space.

    abstract::1. The role of the structures of the outer ear in producing monaural and binaural spectral cues to sound location was examined acoustically in the ferret. A probe microphone was introduced across the wall of the external auditory canal and its responses to digitally constructed wideband signals were recorded for a lar...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.71.2.785

    authors: Carlile S,King AJ

    更新日期:1994-02-01 00:00:00

  • Spatiotemporal organization of cat lateral geniculate receptive fields.

    abstract::Spatial and temporal properties of LGN receptive fields were studied by flashing a small bar of light across the field in 28 discrete steps. The flashes at each of the spatial positions were used to produce 28 PST histograms. These histograms were in turn displayed as a plane, with space on the chi axis, time on the p...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1976.39.2.213

    authors: Stevens JK,Gerstein GL

    更新日期:1976-03-01 00:00:00

  • Responsiveness to D-glucose in neurosecretory cells of crustaceans.

    abstract::1. An electrophysiological study of the D-glucose sensitivity of X-organ (XO) neurosecretory cell bodies in crayfish was carried out with the use of microelectrodes, perforated, and cell-attached patch-clamp techniques. 2. Glucose depolarizes the membrane potential of XO cells in a concentration-dependent manner. 3. D...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.70.2.758

    authors: García E,Benítez A,Onetti CG

    更新日期:1993-08-01 00:00:00

  • Modulation of the human vestibuloocular reflex during saccades: probing by high-frequency oscillation and torque pulses of the head.

    abstract::1. We probed the gain and phase of the vestibuloocular reflex (VOR) during the execution of voluntary gaze saccades, with continuous oscillation or acceleration pulses, applied through a torque helmet. 2. Small-amplitude (< 1 degree), high-frequency (10-14 Hz) head oscillations in the horizontal or vertical plane were...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.76.5.3249

    authors: Tabak S,Smeets JB,Collewijn H

    更新日期:1996-11-01 00:00:00

  • Differential gurmarin suppression of sweet taste responses in rat solitary nucleus neurons.

    abstract::We examined the effect of the sweet transduction blocker gurmarin on taste responses recorded from neurons in the rat solitary nucleus (NST) to determine how gurmarin sensitivity is distributed across neuronal type. Initially, responses evoked by washing the anterior tongue and palate with 0.5 M sucrose, 0.1 M NaCl, 0...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00215.2003

    authors: Lemon CH,Imoto T,Smith DV

    更新日期:2003-08-01 00:00:00

  • Homeostatic mechanisms may shape the type and duration of oscillatory modulation.

    abstract::Neural oscillations are observed ubiquitously in the mammalian brain, but their stability is known to be rather variable. Some oscillations are tonic and last for seconds or even minutes. Other oscillations appear as unstable bursts. Likewise, some oscillations rely on excitatory AMPAergic synapses, but others are GAB...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00119.2020

    authors: Peterson EJ,Voytek B

    更新日期:2020-07-01 00:00:00