Abstract:
:GH promotes the conversion of cultured preadipose 3T3 cells into adipose cells. The serum of most animals also promotes this differentiation. In order to determine the extent to which GH is responsible for the adipogenic activity of serum, we used a specific antiserum able to suppress completely the adipogenic activity of rat and bovine GH. This antiserum also suppressed all the adipogenic activity secreted into culture medium by a rat pituitary cell line as well as all the adipogenic activity of crude bovine pituitary extract. When tested against the adipogenic activity of serum, the antiserum to GH reduced the activity by one third to half. It is concluded that 1) GH in the concentration and form present in serum is an effective promoter of adipose differentiation, and 2) there exists in serum another adipogenic activity not immunochemically recognizable as GH. This activity is presumed to be of nonpituitary origin.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Nixon T,Green Hdoi
10.1210/endo-114-2-527subject
Has Abstractpub_date
1984-02-01 00:00:00pages
527-32issue
2eissn
0013-7227issn
1945-7170journal_volume
114pub_type
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