Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cells.

abstract：:A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and...

journal_title：Endocrinology

authors： Husain A,Smeby RR,Wilk D,Dzau VJ,Bumpus FM

更新日期：1984-06-01 00:00:00

abstract：:The maximum amounts of angiotensins II and III that were confined to the kidney during intrarenal arterial administration in conscious dogs were determined to be 2 and 12 pmol/kg X min, respectively. These doses were infused chronically intrarenally to uninephrectomized dogs. A significant reduction in urinary volume ...

journal_title：Endocrinology

authors： Dickstein GM,Woodson JF,Lamb NE,Rose CE Jr,Peach MJ,Carey RM

更新日期：1985-11-01 00:00:00

abstract：:Spontaneously hypertensive (SH) rats, extensively used as experimental models of essential human hypertension, display important alterations in the neuroendocrine reproductive axis, which manifest as markedly delayed puberty onset in females but whose basis remains largely unknown. We analyze herein in female SH rats:...

journal_title：Endocrinology

authors： Pinilla L,Castellano JM,Romero M,Tena-Sempere M,Gaytán F,Aguilar E

更新日期：2009-06-01 00:00:00

abstract：:Activin A is a member of the transforming growth factor-beta family of growth factors and a potent regulator of cellular activity. A number of binding proteins for activin A have been identified, including alpha 2-macroglobulin (alpha 2M). Alpha 2M has several conformational states that are known to have different gro...

journal_title：Endocrinology

authors： Niemuller CA,Randall KJ,Webb DJ,Gonias SL,LaMarre J

更新日期：1995-12-01 00:00:00

abstract：:The adipokine resistin is an insulin-antagonizing factor that also plays a regulatory role in inflammation, immunity, food intake, and gonadal function. Although adipose tissue is the primary source of resistin, it is also expressed in other tissues and organs, including the pituitary. However, there is no information...

journal_title：Endocrinology

authors： Rodríguez-Pacheco F,Vázquez-Martínez R,Martínez-Fuentes AJ,Pulido MR,Gahete MD,Vaudry H,Gracia-Navarro F,Diéguez C,Castaño JP,Malagón MM

更新日期：2009-10-01 00:00:00

abstract：:The insulin-like growth factors (IGF)-I and -II are bound to specific carrier proteins in the circulation. For investigation of their physiological role, the acid-stable subunit of the major binding protein (SmBP) was isolated from human plasma Cohn fraction IV. Its effect on the mitogenic activity of IGF-I was studie...

journal_title：Endocrinology

authors： Blum WF,Jenne EW,Reppin F,Kietzmann K,Ranke MB,Bierich JR

更新日期：1989-08-01 00:00:00

abstract：:Attempts to assess human placental GH variant (hGH-V) and chorionic somatomammotropin (hCS) RNA in choriocarcinoma cell lines have been hampered by low levels of expression and limited sensitivity of RNA blotting analysis. We examined human choriocarcinoma BeWo, JAR, and JEG-3 cell lines as well as samples of complete...

journal_title：Endocrinology

authors： Lytras A,Bock ME,Dodd JG,Cattini PA

更新日期：1994-06-01 00:00:00

abstract：:Anandamide and 2-arachidonoylglycerol (2-AG), two endogenous ligands of the CB1 and CB2 cannabinoid receptor subtypes, inhibit the proliferation of PRL-responsive human breast cancer cells (HBCCs) through down-regulation of the long form of the PRL receptor (PRLr). Here we report that 1) anandamide and 2-AG inhibit th...

journal_title：Endocrinology

authors： Melck D,De Petrocellis L,Orlando P,Bisogno T,Laezza C,Bifulco M,Di Marzo V

更新日期：2000-01-01 00:00:00

abstract：:Male Sprague-Dawley rats were infused continuously for 13 days with vehicle or 75 pmol (31.2 ng)/day 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] by means of Alzet osmotic minipumps implanted s.c. Animals infused with 1,25-(OH)2D3 exhibited mild hypercalcemia (11.2 vs. 10.2 mg/dl in controls), a 136% increase in the serum ...

journal_title：Endocrinology

authors： Wronski TJ,Halloran BP,Bikle DD,Globus RK,Morey-Holton ER

更新日期：1986-12-01 00:00:00

abstract：:This paper describes experiments on cultured granulosa cells isolated from ovaries of immature rats designed to locate the site of action of androgens on FSH-induced aromatase activity. Treatment of cells during a 36-h induction period with (Bu)2cAMP, 8- BrcAMP , FSH, prostaglandin E2, or cholera toxin resulted in ind...

journal_title：Endocrinology

authors： Daniel SA,Armstrong DT

更新日期：1984-06-01 00:00:00

abstract：:Circulating levels of the pancreatic beta-cell peptide hormone amylin and the gut peptide PYY[3-36] increase after nutrient ingestion. Both have been implicated as short-term signals of meal termination with anorexigenic and weight-reducing effects. However, their combined effects are unknown. We report that the combi...

journal_title：Endocrinology

authors： Roth JD,Coffey T,Jodka CM,Maier H,Athanacio JR,Mack CM,Weyer C,Parkes DG

更新日期：2007-12-01 00:00:00

abstract：:Glucose release from the liver is mediated by hypothalamic norepinephrine (NE) neuronal activity, but glucose itself (or a metabolite of it) exerts negative feedback effects on central NE activity. The aim of the present study was to investigate a possible role for insulin in these central glucose homeostatic mechanis...

journal_title：Endocrinology

authors： Smythe GA,Bradshaw JE,Nicholson MV,Grunstein HS,Storlien LH

更新日期：1985-10-01 00:00:00

abstract：:Lanosterol 14alpha-demethylase (CYP51) responds to cholesterol feedback regulation through sterol regulatory element binding proteins (SREBPs). The proximal promoter of CYP51 contains a conserved region with clustered regulatory elements: GC box, cAMP-response elements (CRE-like), and sterol regulatory element (SRE). ...

journal_title：Endocrinology

authors： Fink M,Acimovic J,Rezen T,Tansek N,Rozman D

更新日期：2005-12-01 00:00:00

abstract：:Intermittent PTH administration increases bone turnover, resulting in net anabolic effects on bone. These effects are primarily mediated by intracellular cAMP signaling. However, the molecular mechanisms that regulate PTH activity in bone remain incompletely understood. beta-Arrestin2, a G protein-coupled receptor reg...

journal_title：Endocrinology

authors： Ferrari SL,Pierroz DD,Glatt V,Goddard DS,Bianchi EN,Lin FT,Manen D,Bouxsein ML

更新日期：2005-04-01 00:00:00

abstract：:Relaxin is a naturally occurring regulator of collagen turnover. In this study, we determined the role of endogenous relaxin in the pathogenesis of primary tubulointerstitial fibrosis after unilateral ureteric obstruction (UUO). Four- to 6-wk-old relaxin (RLX) gene-knockout (RLX(-/-)) and age-matched wild-type (RLX(+/...

journal_title：Endocrinology

authors： Hewitson TD,Mookerjee I,Masterson R,Zhao C,Tregear GW,Becker GJ,Samuel CS

更新日期：2007-02-01 00:00:00

abstract：:In order to compare, in vitro, the TSH suppressive effects of iodothyronines, rat pituitary quarters were first preincubated with T4, T3, rT3, or 3,3'-diiodothyronine (T2) in Gey and Gey buffer containing 1% bovine serum albumin for 2 h at 37 C and then incubated at 37 C for 1 h with the iodothyronine under study and ...

journal_title：Endocrinology

authors： Chopra IJ,Carlson HE,Solomon DH

更新日期：1978-08-01 00:00:00

abstract：:We are investigating the mechanisms by which aromatic hydrocarbons, such as 3-methylcholanthrene (MC), suppress hepatic cytochrome P450 2C11 (CYP2C11) gene expression. CYP2C11 is an enzyme expressed in the liver of male rats and is regulated by a pulsatile pattern of GH secretion. We have previously shown that MC atte...

journal_title：Endocrinology

authors： Timsit YE,Riddick DS

更新日期：2002-09-01 00:00:00

abstract：:Flavonoids are functional constituents of many fruits and vegetables. Some flavonoids have antidiabetic properties because they improve altered glucose and oxidative metabolisms of diabetic states. Procyanidins are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological ox...

journal_title：Endocrinology

authors： Pinent M,Blay M,Bladé MC,Salvadó MJ,Arola L,Ardévol A

更新日期：2004-11-01 00:00:00

abstract：:The expression and functions of receptors for the beta-chemokine, regulated upon activation, normal T cell expressed, and secreted (RANTES)/CCL5, were investigated in osteoblasts. Both primary osteoblasts and the MC3T3-E1 osteoblast cell line express the RANTES receptors, CCR1, 3, 4, and 5 (by RT-PCR), which encode fu...

journal_title：Endocrinology

authors： Yano S,Mentaverri R,Kanuparthi D,Bandyopadhyay S,Rivera A,Brown EM,Chattopadhyay N

更新日期：2005-05-01 00:00:00

abstract：:Mature 31- and 34-kDa inhibin A and B negatively regulate the release of FSH from the anterior pituitary; however, a direct comparison of these hormones in vivo has not been undertaken. The bioactivities of highly purified preparations of recombinant human 31-kDa inhibin A and B were determined in rat pituitary cells ...

journal_title：Endocrinology

authors： Makanji Y,Temple-Smith PD,Walton KL,Harrison CA,Robertson DM

更新日期：2009-10-01 00:00:00

abstract：:In Teleost fish, development, growth, and reproduction are influenced by the daily and seasonal variations of photoperiod and temperature. Early in vivo studies indicated the pineal gland mediates the effects of these external factors, most probably through the rhythmic production of melatonin. The present investigati...

journal_title：Endocrinology

authors： Falcón J,Besseau L,Fazzari D,Attia J,Gaildrat P,Beauchaud M,Boeuf G

更新日期：2003-10-01 00:00:00

abstract：:Metalloproteinase inhibitors, such as tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and -2), play a key role in the regulation of metalloproteinases that modify extracellular matrix composition. Although expression of TIMP-1 within ovarian tissues has been well characterized, little information is available ...

journal_title：Endocrinology

authors： Smith GW,McCrone S,Petersen SL,Smith MF

更新日期：1995-02-01 00:00:00

abstract：:Chloroquine is widely used as a lysosomal enzyme inhibitor, and while effects on DNA repair and membrane recycling have also been reported, there has been no investigation of effects on the processing of secretory products. To determine whether chloroquine had any effect on secretion and secretory organelles, we monit...

journal_title：Endocrinology

authors： Conconi MV,Walker AM

更新日期：1984-03-01 00:00:00

abstract：:The antitumor properties of antibodies to the beta-subunit of hCG (hCG beta) coupled to tetanus toxoid (tt) were studied in hamsters implanted with the BeWo strain of human choriocarcinoma. After active immunization with hCG beta-tt mixed in complete Freund's adjuvant, hamsters synthesized large quantities of antibodi...

journal_title：Endocrinology

authors： Knecht M

更新日期：1980-01-01 00:00:00

abstract：:We looked for the presence of vitamin D-dependent intestinal calcium binding protein (CaBP), relative molecular mass (mol wt) about 10,000, in kidneys of mammals, birds, and reptiles, and compared the localization of this 10,000 mol wt CaBP with the localization of the 28,000 mol wt vitamin D-dependent CaBP. Antiserum...

journal_title：Endocrinology

authors： Rhoten WB,Bruns ME,Christakos S

更新日期：1985-08-01 00:00:00

abstract：:Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. Gremlin, a BMP antagonist, is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. However, its effects in vivo are not known. To investigate the actions of gremlin on bone remodeling in...

journal_title：Endocrinology

authors： Gazzerro E,Pereira RC,Jorgetti V,Olson S,Economides AN,Canalis E

更新日期：2005-02-01 00:00:00

abstract：:The secretion of LH, PRL, and cortisol was investigated in 4 sexually mature female rhesus macaques with cardiac catheters protected by tethers. Based on endocrine parameters, all 4 of the animals ovulated within 2 months from the time they were tethered, and regular menstrual cycles of 24-34 days were observed. The c...

journal_title：Endocrinology

authors： Norman RL,Lindstrom SA,Bangsberg D,Ellinwood WE,Gliessman P,Spies HG

更新日期：1984-07-01 00:00:00

abstract：:Protein kinase-C (PKC) has been suggested as a possible mediator of the antigonadotropic action of prostaglandin F2 alpha (PGF2 alpha) in luteal cells. To examine this possibility, we evaluated the effects of phorbol ester [12-O-tetradecanoylphorbol-13-acetate (TPA)] in relation to those of PGF2 alpha on cAMP accumula...

journal_title：Endocrinology

authors： Musicki B,Aten RF,Behrman HR

更新日期：1990-03-01 00:00:00

abstract：:The osteoclast is known to be derived from the hemopoietic stem cell, but its lineage and the mechanisms by which its differentiation is regulated are largely unknown. There is evidence that osteoclastic differentiation is induced through a contact-dependent interaction between bone marrow stromal cells and hemopoieti...

journal_title：Endocrinology

authors： Hattersley G,Kerby JA,Chambers TJ

更新日期：1991-01-01 00:00:00

abstract：:This study investigates the signaling pathways that lead to acute augmentation of secretagogue-induced LH secretion, the physiologically relevant manifestation of which is LHRH self-potentiation. The consequence of LHRH self-potentiation is an augmented LH secretory response to subsequent exposure to the peptide. Alth...

journal_title：Endocrinology

authors： Waring DW,Turgeon JL

更新日期：1992-06-01 00:00:00