Abstract:
:The osteoclast is known to be derived from the hemopoietic stem cell, but its lineage and the mechanisms by which its differentiation is regulated are largely unknown. There is evidence that osteoclastic differentiation is induced through a contact-dependent interaction between bone marrow stromal cells and hemopoietic precursors. To analyze osteoclastic lineage, colonies were generated in semi-solid medium from mouse spleen cells in the presence of erythropoietin with either Wehi 3B-conditioned medium or interleukin 3 (IL3). After 7 days, individual colonies were picked. Half of each colony was phenotyped by the morphology of cells in cytospin preparations; the second half of each was incubated for 7 days with a bone marrow-derived cell line (ts8) that induces osteoclastic differentiation from hemopoietic cells, on bone slices in the presence of 1,25-dihydroxyvitamin D3. After incubation, bone resorption was assessed by scanning electron microscopy. No resorption was induced in cells derived from single-lineage colonies, but resorptive cells differentiated in 17% of granulocyte-macrophage (GM) colonies and 38% of multilineage colonies. Since only a minority of GM colonies contained osteoclastic precursors, this suggests that the GM colonies that contained osteoclasts were not typical GM colonies but may have been a form of multilineage colony analagous to other multilineage colonies that contain granulocytes, macrophages, and a third cell type. No resorptive cells were formed when IL3-derived colonies were incubated on bone slices without ts8 cells. The results suggest that osteoclasts are derived from a multilineage precursor, upon which IL3 acts to generate cells capable of osteoclastic differentiation, which form resorptive cells upon incubation with bone marrow stromal cells in the presence of 1,25-dihydroxyvitamin D3.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Hattersley G,Kerby JA,Chambers TJdoi
10.1210/endo-128-1-259subject
Has Abstractpub_date
1991-01-01 00:00:00pages
259-62issue
1eissn
0013-7227issn
1945-7170journal_volume
128pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Studies were performed to characterize the identity of the factors present in calf serum that stimulate endothelin (ET) production by vascular endothelial cells in culture. Two putative candidates responsible for the ET-releasing activity present in serum are members of the transforming growth factor-beta (TGF beta) s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2355
更新日期:1991-11-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) is a multifunctional cytokine that is thought to play a major role in the regulation of growth and differentiation of thyroid cells. However, little is known of its detailed mechanisms of action in thyrocytes. We have therefore studied the molecular mechanisms of TGF-beta1 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7918
更新日期:2001-01-01 00:00:00
abstract::We evaluated the capacity of estradiol (E(2)) to regulate PTHrP production, cell growth, tumor growth, and metastasis to the skeleton in breast cancer. In estrogen receptor (ER)-negative human breast cancer cells, MDA-MB-231, and cells transfected with full-length cDNA encoding ER (S-30), E(2) caused a marked decrease...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0062
更新日期:2005-07-01 00:00:00
abstract::Bovine placental lactogen (bPL) was found to be as potent as human GH (hGH) in its ability to bind to soluble full-size recombinant hGH-binding protein (hGHBP) and to membrane-embedded hGH receptor in intact IM-9 human lymphocytes. bPL was also capable of forming a 1:2 complex with hGHBP, although the structure of thi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867580
更新日期:1995-03-01 00:00:00
abstract::PTH stimulates synthesis and secretion of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in renal proximal tubule cells through activation of the protein kinase-A (PKA) or the protein kinase-C (PKC) signaling pathway. The relative contribution of the two transducing systems was explored using PTH fragments with selective act...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8344210
更新日期:1993-08-01 00:00:00
abstract::As ovariectomy induces obesity in rats, we have investigated the influence of ovariectomy and hormone replacement on the proliferation and differentiation capacities of rat cultured preadipocytes removed from different fat depots (femoral sc, parametrial, and perirenal). Ovariectomy induced increased proliferation and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5246
更新日期:1997-07-01 00:00:00
abstract::Androgen signaling in prostate smooth muscle cells (pSMCs) is critical for the maintenance of prostate homeostasis, the alterations of which are a central aspect in the development of pathological conditions. Testosterone can act through the classic androgen receptor (AR) in the cytoplasm, eliciting genomic signaling,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00718
更新日期:2018-02-01 00:00:00
abstract::The major changes in pancreatic islet function during pregnancy and after exposure to lactogens are an increase in beta-cell proliferation and enhanced insulin secretion. In this study we examined INS-1 cells as a potential model for further inquiry into PRL signaling in beta-cells. Proliferation of beta-cells, insuli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.4.5089
更新日期:1997-04-01 00:00:00
abstract::We recently reported that aluminum administration to beagles stimulates uncoupled bone formation in the marrow cavity which increases trabecular bone volume and generates new osseous networks within the axial skeleton. To investigate whether this osteogenic process results from direct stimulation of bone cell replicat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3144
更新日期:1991-06-01 00:00:00
abstract::Resorption of devitalized bone particles by monocytes grown in culture was stimulated by platelet-derived growth factor (PDGF) in a dose-dependent manner. Bone resorption in response to PDGF was time-dependent with a significant increase over control cultures evident by 72 hours. These data are the first to demonstrat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-2-761
更新日期:1983-02-01 00:00:00
abstract::Osteocalcin, a synthetic osteoblast-specific protein, has recently emerged as an important regulator of energy metabolism, but the underlying mechanisms are not fully understood. In the present study, mice fed a high-fat diet and receiving osteocalcin showed reduced body weight gain, less fat pad gain, and improved in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2144
更新日期:2013-03-01 00:00:00
abstract::Task3 K(+) channels are highly expressed in the adrenal cortex and contribute to the angiotensin II and K(+) sensitivity of aldosterone-producing glomerulosa cells. Adult Task3(-/-) mice display a partially autonomous aldosterone secretion, subclinical hyperaldosteronism, and salt-sensitive hypertension. Here, we inve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1101
更新日期:2013-08-01 00:00:00
abstract::The mineralocorticoid receptor (MR), a member of the nuclear receptor superfamily of transcription factors, is activated by aldosterone and mediates its natriferic action in tight epithelia. MR is also expressed in nonepithelial tissues. Importantly, it mediates the deleterious effects of inappropriately high aldoster...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1210
更新日期:2012-07-01 00:00:00
abstract::The effect of osmotic changes on aldosterone production, [Ca2+]i and voltage-gated Ca2+ currents, was studied in cultured rat glomerulosa cells. Alteration of osmolarity by sucrose addition in the 250-330 mosM range did not influence aldosterone production per se, but it substantially affected K+-stimulated aldosteron...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7465
更新日期:2000-05-01 00:00:00
abstract::Altered leptin action has been implicated in the pathophysiology of heart failure in obesity, a hallmark of which is extracellular matrix remodeling. Here, we characterize the direct influence of leptin on matrix metalloproteinase (MMP) activity in primary adult rat cardiac fibroblasts and focus on elucidating the mol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1166
更新日期:2011-05-01 00:00:00
abstract::Suppression of spermatogenesis in LBNF1 rats by treatment with the GnRH agonist Zoladex combined with the antiandrogen flutamide was evaluated in order to rapidly achieve protection of spermatogenic stem cells against procarbazine with clinically used drugs. Zoladex-flutamide treatment required 3 weeks to suppress the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628410
更新日期:1995-08-01 00:00:00
abstract::Prostaglandin (PG) E2 (PGE(2)) plays a central role in the regulation of smooth muscle contractions. Classically, PGE(2) stimulates contractions via EP1 and EP3 receptors, whereas EP2 and EP4 maintain quiescence. Labor involves a change from myometrial quiescence to contractions with a shift from anti- to proinflammat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1761
更新日期:2014-02-01 00:00:00
abstract::The proglucagon gene (glu) encodes the incretin hormone glucagon-like peptide-1 (GLP-1), produced in the intestinal endocrine L cells. We found previously that the bipartite transcription factor beta-catenin/T cell factor (cat/TCF), the major effector of the canonical Wnt signaling pathway, activates intestinal glu ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1142
更新日期:2008-05-01 00:00:00
abstract::TSH is the most important biomarker in the interpretation of thyroid function in man. Its levels are determined by circulating thyroid hormone (TH) levels that feed back centrally to regulate the expression of the subunits that comprise TSH from the pituitary. The nuclear corepressor 1 (NCoR1), is a critical coregulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1504
更新日期:2012-10-01 00:00:00
abstract::Exposure to environmental toxicants during fetal development alters gene expression and promotes disease later in life. Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer widely used for the manufacturing of consumer products. Exposure to DEHP has been associated with obesity, asthma, and low T levels. In utero expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1436
更新日期:2015-01-01 00:00:00
abstract::Oral ketoconazole has been demonstrated to lower plasma testosterone in man. Measurement of blood precursors of testosterone suggest that ketoconazole may have its effect inhibiting the 17,20-desmolase enzyme within the testis. To substantiate this, a series of in vitro experiments was conducted using the rat testis t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-5-1920
更新日期:1985-05-01 00:00:00
abstract::Diagnostic tests of hypothalamic-pituitary-adrenocortical function in psychiatric illness largely report the interaction of hypothalamic secretagogues with glucocorticoids at the pituitary level. This study investigated whether the efficiency of glucocorticoid inhibition is subject to modulation by intracellular proce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220489
更新日期:2002-10-01 00:00:00
abstract::The control of ACTH secretion by opiates seems to involve stimulatory and inhibitory pathways, since opiate agonists and antagonists are capable of releasing ACTH in conscious rats. To elucidate the role of different opiate receptors in the control of ACTH release, rats were treated with receptor-selective opiate agon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2688
更新日期:1985-06-01 00:00:00
abstract::An estrogen-regulated arginine esteropeptidase is present in the immature rat uterus. The enzymatic complex consists of a membrane-bound activator and a soluble proenzyme. The activator is under strong estrogen control; its activity increases 10-fold 3 h after a single dose of 17 beta-estradiol. The subcellular locali...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-500
更新日期:1988-02-01 00:00:00
abstract::The Pax6 transcription factor is crucial for pancreatic α-cells. Indeed, Pax6-deficient mouse models are characterized by markedly altered α-cell differentiation. Our objective was to investigate the role of Pax6 in glucagon secretion process. We used a Pax6-deficient model in rat primary enriched-α cells with specifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1425
更新日期:2012-09-01 00:00:00
abstract::Mutations in the GnRH receptor gene (GNRHR) can result in hypogonadotropic hypogonadism in humans. Unlike most mammals, mice lack a second form of GnRH (GnRH2) and a type 2 GnRH receptor. To determine whether the GnRH receptor is critical at all stages of reproduction and whether this receptor has additional physiolog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0598
更新日期:2010-03-01 00:00:00
abstract::Extracts of rat pancreas contain significant amounts of an [3H]estradiol-binding protein. The amount of steroid-binding activity that could be measured varied considerably depending on the tonicity of the homogenizing medium. High speed supernatants of homogenates initially prepared in isotonic buffer contained about ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2221
更新日期:1985-06-01 00:00:00
abstract::The estrogenic and antiestrogenic actions of the geometric isomers of tamoxifen and 4-hydroxytamoxifen were determined in a PRL synthesis assay using primary cultures of dispersed immature rat pituitary gland cells. 4-Hydroxytamoxifen was 100 times more potent as an antiestrogen than tamoxifen. The cis isomer of tamox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1449
更新日期:1988-04-01 00:00:00
abstract::1Alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) is a potent mediator of differentiation and maintenance of specific functions of osteoblasts. To detect novel targets for 1,25-(OH)2D3 action, we applied differential display PCR to human fetal osteoblast-like cells and identified the human analog of murine cystein rich pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5954
更新日期:1998-04-01 00:00:00
abstract::The xenoestrogen bisphenol A (BPA) has been shown to mimic estrogen both in vivo and in vitro. BPA stimulates PRL secretion and the expression of a PRL regulating factor from the posterior pituitary in the estrogen-sensitive Fischer 344 rat (F344), but not in Sprague-Dawley (SD) rats. The goal of the present studies w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6027
更新日期:1998-06-01 00:00:00