Abstract:
:The effects of food restriction of the mother (65% restriction of ad libitum food intake) on fetal and maternal insulin secretion and islet function were studied at 21 days gestation in three different rat populations: 1) undernourished from 0-7 days gestation, 2) undernourished from 7-14 days gestation, and 3) undernourished from 14-21 days gestation. The body weights of mothers were decreased in groups 2 and 3 vs. those in control fed pregnant animals, and no changes in basal parameters were found in any group. A glucose tolerance test in mothers from group 3 showed a mild intolerance to glucose and a decreased islet insulin content, although islet stimulation in vitro with glucose alone or plus arginine showed a normal insulin secretory response. Body weight was decreased in fetuses from the three groups (P < 0.01), and pancreas weight was reduced only in group 3. Insulinemia was increased in groups 2 and 3, and pancreatic insulin content increased only in group 3. However, fetuses from mothers of group 3 showed increased islet insulin content, increased response of insulin in vitro to glucose or glucose plus arginine, and hypertrophy of beta-cell mass. These results indicate, first, that the development of the fetal pancreas depends on a balanced maternal glucose homeostasis and, second, that adaptive maternal changes to undernutrition seem to induce alterations on the fetal endocrine pancreas, especially when food restriction is applied during the last week of gestation.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Alvarez C,Martín MA,Goya L,Bertin E,Portha B,Pascual-Leone AMdoi
10.1210/endo.138.6.5202subject
Has Abstractpub_date
1997-06-01 00:00:00pages
2267-73issue
6eissn
0013-7227issn
1945-7170journal_volume
138pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Cyclic somatostatin strongly stimulated secretion of histamine from rat peritoneal mast cells. The energy, temperature and calcium dependence of this effect indicate that the action of somatostatin on the mast cells is similar to that of the classic mast cell secretagogue compound 48/80 and is to induce exocytosis. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-5-1637
更新日期:1978-05-01 00:00:00
abstract::LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867570
更新日期:1995-03-01 00:00:00
abstract::Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ET(A)R) in murine models of thyroid carcinoma using optical imaging methods. A...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-2017
更新日期:2012-06-01 00:00:00
abstract::Vasoactive intestinal polypeptide (VIP)ergic nerves innervate both the neurohypophysis and the hypothalamus. To test the hypothesis that VIP is a releasing factor for neurohypophyseal hormones, rats were given intracerebroventricular (icv) infusions of VIP in doses varying from 0.3 pmol/kg.min to 3 nmol/kg.min for 5 m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2249
更新日期:1988-11-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) play potential roles in chemoprevention of colon cancer and others by inhibiting prostaglandin synthesis. In this report, we used LNCaP cells, an androgen-responsive human prostate carcinoma cell line, to study the effects of two NSAIDs, flufenamic acid (FA) and piroxicam ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7246
更新日期:1999-11-01 00:00:00
abstract::The Leydig cells of the testis are known to possess high affinity receptors for luteinizing hormone and human chorionic gonadotropin (hCG), but no information concerning the synthesis of these receptors is available yet. In order to investigate this question, we have purified crude rat interstitial cell preparations o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2129
更新日期:1982-12-01 00:00:00
abstract::The effects of electrical stimulation of the brain on the ovarian venous plasma concentrations of estradiol (E2) and progesterone (P) were investigated in female rats hypophysectomized and adrenalectomized at 1000 h on the day of proestrus. Stimulation was applied during the proestrous critical period under pentobarbi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-136
更新日期:1981-07-01 00:00:00
abstract::During in vitro maturation of porcine cumulus cell-oocyte complexes and in vitro luteinization of porcine granulosa cells, FSH induces the expression of the protease TNFα-converting enzyme/A disintegrin and metalloproteinase domain 17 (TACE/ADAM17) and the epidermal growth factor (EGF)-like factors, which activate the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1655
更新日期:2014-03-01 00:00:00
abstract::The receptor for the orexigenic peptide, ghrelin, is one of the most constitutively active 7TM receptors known, as demonstrated under in vitro conditions. Change in expression of a constitutively active receptor is associated with change in signaling independent of the endogenous ligand. In the following study, we fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1638
更新日期:2009-11-01 00:00:00
abstract::To study the interaction of TSH receptor (TSHR) autoantibodies with receptor protein; it is necessary first to express the receptor in the proper conformation including the formation of correct disulfide bridges. However, the reducing environment of the Escherichia coli (E. coli) cytoplasm prevents the generation of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4920
更新日期:1997-02-01 00:00:00
abstract::Kisspeptins are new actors in the neuroendocrine regulation of reproduction. In vertebrates, the number of kiss genes varies from none to three. Zebrafish have two kiss genes, kiss1 and kiss2, and two kiss receptors (GPR54), kiss1r and kiss2r. To provide detailed information on the organization of the kiss systems in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0948
更新日期:2011-04-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::We have investigated the production and actions of a growth regulatory protein, insulin-like growth factor binding protein (IGFBP)-3, in the androgen-responsive prostate carcinoma cell line LNCaP. Confluent monolayers of cells secreted approximately 0.7 ng/ml IGFBP-3 over 24 h. Dihydrotestosterone (DHT, 10 nM) and 1,2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.7.7534
更新日期:2000-07-01 00:00:00
abstract::Pancreatic membrane receptors for cholecystokinin (CCK) in obese and nonobese Zucker rats were compared with the use of a biologically active [125I]iodo-CCK-8 radioprobe. Membrane homogenates from obese rats bound half the amount of radioligand in 2 h as did membranes from lean rats (specifically bound, 7.0% vs. 14.0%...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-546
更新日期:1986-08-01 00:00:00
abstract::Osteocalcin, a synthetic osteoblast-specific protein, has recently emerged as an important regulator of energy metabolism, but the underlying mechanisms are not fully understood. In the present study, mice fed a high-fat diet and receiving osteocalcin showed reduced body weight gain, less fat pad gain, and improved in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2144
更新日期:2013-03-01 00:00:00
abstract::The present study was undertaken to determine whether T3 could modify anterior pituitary Ca++ metabolism under basal conditions and in the presence of TRH. Paired hemipituitaries from hypothyroid rats were preincubated with T3 (10(-7) M) for 2 h, allowed to accumulate 45Ca++ for the third hour, and repeatedly washed i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1690
更新日期:1981-05-01 00:00:00
abstract::Nitric oxide (NO) has emerged as an intracellular and intercellular messenger in a number of biological systems. In the present study, we demonstrated that NO was produced by cultured rat Leydig cells, and that inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) was expressed in Leydig cells. NO was measured a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4961
更新日期:1997-03-01 00:00:00
abstract::The binding characteristics of 125I-labeled Leu15-gastrin and the molecular identification of the gastrin receptor was investigated in dispersed guinea pig gastric glands. The binding of [125I]gastrin to gastric glands was temperature dependent, saturable, and specific. At 37 C, the binding was rapid, became maximal w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-1881
更新日期:1989-04-01 00:00:00
abstract::FSH promotes the differentiation of ovarian follicular granulosa cells via a cAMP-dependent mechanism. Based upon the presence of a prominent phospholipid/diolein/Ca2+-independent myelin basic protein kinase activity in soluble extracts of proliferating immature rat granulosa cells, we determine whether this activity ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.3.8603610
更新日期:1996-03-01 00:00:00
abstract::The photoreactive analogs of vasopressin, [Phe2,Phe-(p-N3)3]AVP (3a) and [Phe-(p-N3)2]AVP (2a), and the chemically reactive analogs of vasopressin, [Phe2,Phe-(p-NHCOCH2Br)3]AVP (3b) and [Phe-(p-NHCOCH2Br)2]AVP (2c), have been tested in the toad bladder for irreversible stimulation or inhibition of the water permeabili...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-4-1413
更新日期:1983-10-01 00:00:00
abstract::Angiotensin II (AII) receptors in adrenal glomerulosa cells are coupled to adenylate cyclase inhibition. We investigated the importance of cyclase inhibition in adrenal steroidogenesis by treating adrenal glomerulosa cells with the toxin of Bordetella pertussis (20 ng/ml) for 3 and 18 h. This treatment prevented inhib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1697
更新日期:1986-10-01 00:00:00
abstract::The median eminence (ME) region of the hypothalamus was electrically stimulated through permanently implanted electrodes in 1 unanesthetized sheep. Plasma concentrations of GH and prolactin (PRL) were measured at 10-min intervals before, during and after 30-min periods of electrical stimulation or sham procedures. The...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-808
更新日期:1975-10-01 00:00:00
abstract::The roles of growth hormone and somatomedin in stimulating muscle cell proliferation were investigated in a series of experiments on myoblast growth in culture. At levels 100 times normal circulating concentrations, neither growth hormone nor insulin stimulated growth of rat muscle cells in serum-free medium. In contr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-1-32
更新日期:1977-07-01 00:00:00
abstract::To obtain large amounts of hTSH and to study the role of the N-linked oligosaccharides for its biological activity, hTSH was produced using recombinant baculovirus containing the human alpha-subunit and a hTSH beta-minigene, respectively, both under the control of the polyhedrin promoter. Expression in insect cells wa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.1.4897
更新日期:1997-01-01 00:00:00
abstract::The involvement of hypothalamic histaminergic neurons in the stress-induced release of peripheral catecholamines was studied in conscious, freely moving male rats. Blood samples were obtained via a catheter in a femoral artery. Intracerebroventricular infusion of histamine (HA; 30 micrograms) increased the plasma conc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1430
更新日期:1990-03-01 00:00:00
abstract::A central step in the development and sexual differentiation of the brain is the intraneuronal conversion of testosterone to estrogen. This conversion is catalyzed by an enzyme complex comprised of cytochrome P450 aromatase and the ubiquitous NADPH-dependent cytochrome P450 reductase. Brain aromatase activity is devel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.4.7925130
更新日期:1994-10-01 00:00:00
abstract::Somatostatin (SRIF) inhibits GH release from rat somatotropes by reducing adenylate cyclase (AC) activity and the free cytosolic calcium concentration ([Ca(2+)](i)). In contrast, we have reported that SRIF can stimulate GH release in vitro from pig somatotropes. Specifically, 10(-7) and 10(-15) M SRIF stimulate GH rel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8685
更新日期:2002-03-01 00:00:00
abstract::In previous studies we have shown that aromatase cytochrome P450 (P450arom) mRNA and protein increase markedly in luteal tissue between days 10-19 of gestation, whereas cholesterol side-chain cleavage cytochrome P450 (P450scc) appears to be constitutively maintained regardless of hormonal changes occurring during preg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1673
更新日期:1989-09-01 00:00:00
abstract::Actions of protein products resulting from alternative splicing of the Igf1 gene have received increasing attention in recent years. However, the significance and functional relevance of these observations remain poorly defined. To address functions of IGF-I splice variants, we examined the impact of loss of IGF-IEa a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1279
更新日期:2011-05-01 00:00:00
abstract::The preoptic area (POA) of the male dove is a known target area for separable behavioral actions of testosterone and 17 beta-estradiol (E2) and contains an active aromatase system. We have examined the regulatory influence of gonadal hormones on aromatase using an in vitro microassay that measures conversion of [1 alp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2180
更新日期:1986-06-01 00:00:00