Abstract:
:Nonsteroidal anti-inflammatory drugs (NSAIDs) play potential roles in chemoprevention of colon cancer and others by inhibiting prostaglandin synthesis. In this report, we used LNCaP cells, an androgen-responsive human prostate carcinoma cell line, to study the effects of two NSAIDs, flufenamic acid (FA) and piroxicam (PXM), on the cancer cell growth stimulated by androgens. We found that FA had much higher potency to inhibit LNCaP cell growth than PXM. FA dramatically reduced the expression of androgen inducible genes, such as prostate-specific antigen (PSA) and the homeo-domain transcription factor Nkx3.1, but PXM did not. In vitro transfection experiments showed that FA down regulated the PSA expression at the transcription level. Western and northern blot analyses demonstrated that FA inhibited the androgen receptor (AR) expression at mRNA and protein levels. Suppressed AR expression may be the cause of FA-mediated inhibition of the androgen inducible gene expression. Our data also showed that FA significantly reduced the AR promoter-mediated transcription activities. This study indicated that AR might be a target for FA to inhibit LNCaP cell growth. FA and other similar NSAIDs may be potential candidates for chemoprevention of human prostate cancer by modulating the expression of AR.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Zhu W,Smith A,Young CYdoi
10.1210/endo.140.11.7246subject
Has Abstractpub_date
1999-11-01 00:00:00pages
5451-4issue
11eissn
0013-7227issn
1945-7170journal_volume
140pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::GH binding proteins (GHBPs) are present in the blood of several species and by complexing with circulating GH may alter its clearance and distribution. The actions of human GHBP (hGHBP) have hitherto been studied indirectly via their effects on the clearance of hGH. In the present experiments, recombinant preparations...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1396340
更新日期:1992-10-01 00:00:00
abstract::PRL has been reported to exist as a number of mol wt and charge variants. We have attempted to determine the relationships among the charge and lower mol wt variants by two-dimensional gel analysis of pituitary and pituitary cell extracts. Silver-stained gels of the extracts showed three major charge isoforms of 24,00...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1565
更新日期:1985-10-01 00:00:00
abstract::Women with type 1 diabetes are subfertile. Diabetes negatively affects pregnancy by causing early miscarriage and poor prenatal outcomes. In this study we examine consequences of maternal type 1 diabetes on early embryo development, metabolic gene expression, and the pattern of insulin receptor (IR) and IGF-I receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0187
更新日期:2010-09-01 00:00:00
abstract::The effect of gonadotropins on phosphodiesterase activity of rat granulosa cells was studied in an in vitro model. Granulosa cells were prepared from hypophysectomized or intact, estrogen-primed immature female rats and treated with FSH, hCG, or (Bu)2cAMP in vitro. Phosphodiesterase activity was determined in cell hom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-6-2361
更新日期:1984-06-01 00:00:00
abstract::In young adult female rats, the patterns of inhibin subunit mRNA expression during the estrous cycle are regulated by cyclic changes in gonadotropin secretion and follicular development. Since there are distinct alterations in profiles of both hormone secretion and folliculogenesis during the reproductive lifespan of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419130
更新日期:1993-01-01 00:00:00
abstract::A 50-70% depletion in the anterior pituitary concentration of PRL occurred within 5 min of electrical stimulation of a single abdominal mammary nerve of urethane-anesthetized lactating rats previously nonsuckled for 6-8 h. The stimulus parameters were: 1-msec pulses, 10-20/sec at 5-30 V applied 5 sec on and 10 sec off...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-2-458
更新日期:1980-02-01 00:00:00
abstract::Mouse fibroblast cell lines which secrete bovine (b) GH have been generated. This was accomplished by cotransforming mouse L cells (thymidine kinase-negative [TK-] and adenine phosphoribosyl transferase-negative [APRT-]) with DNA molecules encoding the Rous sarcoma virus-long-terminal repeat and bGH genes along with t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1489
更新日期:1986-10-01 00:00:00
abstract::A clonal cell line (44-2C) which synthesizes and secretes somatostatin, neurotensin, calcitonin (CT), and CT gene-related peptide and transiently expresses c-fos was used to characterize the mechanism of action of basic fibroblast growth factor (bFGF). bFGF had two modes of action: 1) short term incubation of 44-2C ce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-3-1121
更新日期:1988-03-01 00:00:00
abstract::Levels of insulin-like growth factor-I (IGF-I), IGF-II, and IGF-binding proteins (IGFBPs) were studied in ovine follicular fluid and serum with a view to determining their respective endocrine and/or paracrine roles in ovarian folliculogenesis. Ovarian follicles of Ile-de-France ewes were dissected and measured indivi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.4.7681760
更新日期:1993-04-01 00:00:00
abstract::Primary cultures of enzymatically dispersed rat anterior pituitary cells were used to examine the effect of pure 31 kilodalton bovine inhibin on GnRH-induced up-regulation of GnRH binding sites. After 2 days in culture, the cells were exposed to stimuli with or without test substances for 10 h, followed by evaluation ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-363
更新日期:1989-01-01 00:00:00
abstract::While uterine stromal cells (USC) appear to modify the function of uterine epithelial cells (UEC) under certain conditions in vivo, relatively little is known about the effect of epithelial cells on stromal cell differentiation and function. To determine if UEC modulate USC function in vitro, highly enriched (> 95%) c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446600
更新日期:1992-12-01 00:00:00
abstract::Although tamoxifen (TAM), a selective estrogen receptor modulator, has been widely used in the treatment of hormone-responsive breast cancer, its estrogen-like effect increases the risk of endometrial cancer. However, the molecular mechanisms of TAM-induced endometrial carcinoma still remain unclear. In this report, w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1607
更新日期:2013-02-01 00:00:00
abstract::Mature 31- and 34-kDa inhibin A and B negatively regulate the release of FSH from the anterior pituitary; however, a direct comparison of these hormones in vivo has not been undertaken. The bioactivities of highly purified preparations of recombinant human 31-kDa inhibin A and B were determined in rat pituitary cells ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1783
更新日期:2009-10-01 00:00:00
abstract::TGF-β plays an important role in the progression of prostate cancer. It exhibits both tumor suppressor and tumor-promoting activities. Correlations between cyclooxygenase (COX)-2 overexpression and enhanced production of prostaglandin (PG)E2 have been implicated in cancer progression; however, there are no studies ind...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2074
更新日期:2013-05-01 00:00:00
abstract::Folliculostellate (FS) cells are known to communicate with each other and with endocrine cells via gap junctions in the anterior pituitary. We investigated whether TGFbeta3 and estradiol, known to regulate FS cell production and secretion of basic fibroblast growth factor (bFGF), increases gap junctional communication...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0122
更新日期:2005-09-01 00:00:00
abstract::The adipokine, leptin (LEP), is a hormonal gateway, signaling energy stores to appetite-regulatory neurons, permitting reproduction when stores are sufficient. Dual-labeling for LEP receptors (LEPRs) and gonadotropins or GH revealed a 2-fold increase in LEPR during proestrus, some of which was seen in LH gonadotropes....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1132
更新日期:2014-10-01 00:00:00
abstract::In several mammalian species, luteinizing hormone-releasing hormone (LHRH) neurons have been shown to migrate from nasal regions to the brain during early development. Using immunocytochemistry, we have identified LHRH containing neurons in developing chick embryos. In embryonic day 4 (E4) and E5 animals, a small grou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1676
更新日期:1991-03-01 00:00:00
abstract::We recently reported that fetal sheep corticotrophs (ACTH-producing cells) at 108 +/- 5 d (days) of gestation are relatively more responsive to CRH than to AVP, whereas those at 139 +/- 0 d (term = 145 d) and in the adult are more responsive to AVP. To further characterize these developmental changes, we used immunocy...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4972
更新日期:1997-03-01 00:00:00
abstract::Leukemia inhibitory factor (LIF) is a pleiotropic cytokine known to control the proliferation and survival of stem cells including primordial germ cells and gonocytes. This led us to study the origin and regulation of testicular LIF. The LIF transcript was detected in the rat testis by RT-PCR from 13.5 days postcoitum...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7399
更新日期:2000-03-01 00:00:00
abstract::The ability of FSH to stimulate the activity of ornithine decarboxylase (ODC) in the ovary of the immature rat and cycling hamster has been examined using specific antisera to gonadotropins. The stimulatory effect of FSH on ODC activity in the ovary of the immature rat was abolished when LH antiserum was administered ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-5-1480
更新日期:1979-05-01 00:00:00
abstract::Most studies of lipoprotein requirements for steroid secretion by the adrenal have examined the mixed cell population of the whole gland; none have examined lipoprotein requirements of guinea pig adrenocortical cells. In this study the effect of exogenous lipoprotein on the ability of cells from each of the different ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-640
更新日期:1987-02-01 00:00:00
abstract::Glucagon-like peptide-2 (GLP-2) is a nutrient-dependent proglucagon-derived hormone that stimulates intestinal growth through poorly understood paracrine and/or neural pathways. The relationship between GLP-2 action and a vagal pathway is unclear. Our aims were to determine whether 1) the GLP-2 receptor (GLP-2R) is ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1232
更新日期:2007-05-01 00:00:00
abstract::T3 and rT3 production rates in the fetus account for roughly only a third of the total T4 production rate; thus, the fate of the majority of T4 produced in the fetus is unknown (the "T4 disposal gap"). We developed sensitive and specific T4 sulfate (T4S) and T3 sulfate (T3S) RIAs to investigate the roles of these comp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1396320
更新日期:1992-10-01 00:00:00
abstract::A [3H]colchicine-binding assay was employed for estimating the relative amounts of soluble and polymerized tubulin present in an individual anterior pituitary lobe. Colchicine binding to these two tubulin pools was time dependent and dose responsive. Scatchard analysis of the binding revealed that [3H]colchicine bound...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-1-114
更新日期:1988-01-01 00:00:00
abstract::A method to prepare suspension cultures of highly viable pinealocytes is described. Pineal glands of neonatal or adult rats can be used; the best yield is obtained from neonatal glands. Morphological examination of the cells indicates that they have a normal, healthy appearance and retain some structures typical of ne...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1483
更新日期:1978-10-01 00:00:00
abstract::The secretory pattern of GH secretion is markedly sexually dimorphic in the adult rat. The patterning of GH secretion is determined by the coordinated activity of somatostatin (SS)- and GH-releasing hormone (GHRH)-containing neurosecretory cells located in the hypothalamus. In this study we examined whether there is s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2369
更新日期:1991-05-01 00:00:00
abstract::C-type natriuretic peptide (CNP), found in endothelial cells, chondrocytes, and neurons, binds its cognate transmembrane receptor, natriuretic peptide receptor-B (NPR-B/GC-B), and stimulates the synthesis of the intracellular signaling molecule, cGMP. The known physiologic consequences of this binding event are vasore...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220702
更新日期:2003-01-01 00:00:00
abstract::Exercise has a potent insulin-sensitivity enhancing effect on skeletal muscle, but the intracellular mechanisms that mediate this effect are not well understood. In muscle, Ras-related C3 botulinum toxin substrate 1 (Rac1) regulates both insulin- and contraction-stimulated glucose transport and is dysregulated in insu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1220
更新日期:2016-08-01 00:00:00
abstract::To investigate the participation of cyclic nucleotides in LHRH-mediated gonadotropin release, cells from the anterior pituitaries of 15-day-old female rats were fractionated on an albumin gradient by sedimentation at unit gravity. gonadotrophs and somatotrophs were detected in the fractions by histology and RIA of hor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-5-1627
更新日期:1980-11-01 00:00:00
abstract::The structural requirements for the inhibition of net bone collagen synthesis by parathyroid hormone (PTH) in vitro have been examined by study of the effects of selected fragments and analogs of bovine PTH (bPTH) upon the incorporation of [3H]proline into collagenase-digestible and -nondigestible proteins by neonatal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-103
更新日期:1981-01-01 00:00:00