Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish.


:Kisspeptins are new actors in the neuroendocrine regulation of reproduction. In vertebrates, the number of kiss genes varies from none to three. Zebrafish have two kiss genes, kiss1 and kiss2, and two kiss receptors (GPR54), kiss1r and kiss2r. To provide detailed information on the organization of the kiss systems in zebrafish, antibodies were raised against the C terminus of zebrafish preproKiss1 and preproKiss2. Immunohistochemistry fully confirmed in situ hybridization data, showing that kiss1-expressing neurons are only located in the habenular nucleus, while kiss2-expressing neurons are found in the dorsal and ventral hypothalamus. Kiss1-expressing cells project only to the interpeduncular and raphe nuclei and strongly expressed the kiss1r receptor. In contrast, kiss2-expressing cells are mostly present in the dorsal and ventral hypothalamus and project widely into the subpallium, the preoptic area, the thalamus, the ventral and caudal hypothalamus, and the mesencephalon. All these regions strongly expressed the kiss2r messengers. Kiss2 fibers profusely innervate the ventral forebrain and notably made close apposition with GnRH3 neurons. Estrogen treatment of juvenile fish with estradiol causes increase in kiss2 and kiss2r expression. In the pituitary gland, no proKiss2- positive fibers were detected, while positive cells were observed in the pars intermedia. In addition to proposing a successful strategy to develop antibodies to kisspeptins, these data indicate that the kiss2 systems of zebrafish are implicated in reproductive events, while the kiss1 gene would play other functions that remain to be established.






Servili A,Le Page Y,Leprince J,Caraty A,Escobar S,Parhar IS,Seong JY,Vaudry H,Kah O




Has Abstract


2011-04-01 00:00:00














  • Extracellularly regulated kinases 1/2 (p44/42 mitogen-activated protein kinases) phosphorylate synapsin I and regulate insulin secretion in the MIN6 beta-cell line and islets of Langerhans.

    abstract::The p44/p42 MAPKs (ERK1/2) cascade regulates beta-cell nuclear events, which modulates cell differentiation and gene transcription, whereas its implication in processes occurring in the cytoplasm, such as activation of the exocytotic machinery, is still unclear. Using the MIN6 beta-cell line and isolated rat islets of...


    pub_type: 杂志文章


    authors: Longuet C,Broca C,Costes S,Hani EH,Bataille D,Dalle S

    更新日期:2005-02-01 00:00:00

  • Physiological and pharmacological inhibitors of luteinizing hormone-dependent steroidogenesis induce heat shock protein-70 in rat luteal cells.

    abstract::Heat shock protein (HSP) synthesis increases in cells with a broad range of stress conditions. We recently showed that induction of HSP-70 is associated with inhibition of hormone-sensitive steroidogenesis, but not hormone-sensitive cAMP accumulation, in rat luteal cells by a mechanism associated with interruption of ...


    pub_type: 杂志文章


    authors: Khanna A,Aten RF,Behrman HR

    更新日期:1995-04-01 00:00:00

  • Reovirus induction of MHC class II antigen in rat thyroid cells.

    abstract::We have previously demonstrated that cultured rat thyroid cells do not exhibit constitutive expression of major histocompatibility (MHC) class II antigens. Using reovirus types 1 and 3, we infected 1B-6 cells (a cloned derivative of the Fisher rat cell line FRTL-5), and found a dose-dependent induction of thyroid cell...


    pub_type: 杂志文章


    authors: Neufeld DS,Platzer M,Davies TF

    更新日期:1989-01-01 00:00:00

  • Angiotensin II/aldosterone dose-response curves in the dog: effect of changes in sodium balance.

    abstract::The possibility that the responsiveness of plasma aldosterone concentration to angiotensin II alters with changes in sodium balance was investigated in male beagle dogs under conditions of controlled sodium and potassium intake. Angiotensin II was infused at four different rates (usually 3, 6, 12, and 24 ng/kg/min), e...


    pub_type: 杂志文章


    authors: Nicholls MG,Tree M,Brown JJ,Douglas BH,Fraser R,Hay GD,Lever AF,Morton JJ,Robertson JI

    更新日期:1978-02-01 00:00:00

  • The parathyroid hormone (PTH)/PTH-related peptide receptor mediates actions of both ligands in murine bone.

    abstract::PTH and PTH-related peptide (PTHrP) have been shown to bind to and activate the same PTH/PTHrP receptor. Recent studies have demonstrated, however, the presence of additional receptors specific for each ligand. We used the PTHrP and PTH/PTHrP receptor gene knock-out models to investigate whether this receptor mediates...


    pub_type: 杂志文章


    authors: Lanske B,Divieti P,Kovacs CS,Pirro A,Landis WJ,Krane SM,Bringhurst FR,Kronenberg HM

    更新日期:1998-12-01 00:00:00

  • Somatostatin stimulates GH secretion in two porcine somatotrope subpopulations through a cAMP-dependent pathway.

    abstract::Somatostatin (SRIF) inhibits GH release from rat somatotropes by reducing adenylate cyclase (AC) activity and the free cytosolic calcium concentration ([Ca(2+)](i)). In contrast, we have reported that SRIF can stimulate GH release in vitro from pig somatotropes. Specifically, 10(-7) and 10(-15) M SRIF stimulate GH rel...


    pub_type: 杂志文章


    authors: Ramírez JL,Gracia-Navarro F,García-Navarro S,Torronteras R,Malagón MM,Castaño JP

    更新日期:2002-03-01 00:00:00

  • Increased Bone Turnover and Possible Accelerated Fracture Healing in a Murine Model With an Increased Circulating C-Type Natriuretic Peptide.

    abstract::Recent studies have revealed that C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth. Nevertheless, the effect of CNP on bone turnover has not yet been well studied. To elucidate this issue, we investigated the bone phenotype of a mouse model with elevated plasma CNP concentrations (SA...


    pub_type: 杂志文章


    authors: Kondo E,Yasoda A,Fujii T,Nakao K,Yamashita Y,Ueda-Sakane Y,Kanamoto N,Miura M,Arai H,Mukoyama M,Inagaki N,Nakao K

    更新日期:2015-07-01 00:00:00

  • Perinatal Exposure to Low-Dose Bisphenol-A Disrupts the Structural and Functional Development of the Hypothalamic Feeding Circuitry.

    abstract::Bisphenol-A (BPA) is a component of polycarbonate and other plastics to which humans are regularly exposed at low levels. BPA is characterized as an endocrine disruptor because of observations of its estrogenic activity in various experimental models. We have previously shown evidence of disrupted hypothalamic feeding...


    pub_type: 杂志文章


    authors: MacKay H,Patterson ZR,Abizaid A

    更新日期:2017-04-01 00:00:00

  • Dexamethasone does not inhibit antidiuretic hormone-stimulated prostaglandin production in vivo.

    abstract::Glucocorticoids are potent inhibitors of prostaglandin (PG) release in vitro, but they fail to influence basal PG production in vivo. To test one possible explanation for this apparent discrepancy in the present study we investigated the effect of dexamethasone (DEX) on basal and antidiuretic hormone-stimulated renal ...


    pub_type: 杂志文章


    authors: Náray-Fejes-Tóth A,Fejes-Tóth G

    更新日期:1986-12-01 00:00:00

  • Hormonal regulation of phospholipid methyltransferase by 3',5'-cyclic adenosine monophosphate-dependent and independent mechanisms.

    abstract::Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effe...


    pub_type: 杂志文章


    authors: Kelly KL,Wong EH

    更新日期:1987-06-01 00:00:00

  • Implication of the Tpl2 kinase in inflammatory changes and insulin resistance induced by the interaction between adipocytes and macrophages.

    abstract::Adipose tissue inflammation is associated with the development of insulin resistance. In obese adipose tissue, lipopolysaccharides (LPSs) and saturated fatty acids trigger inflammatory factors that mediate a paracrine loop between adipocytes and macrophages. However, the inflammatory signaling proteins underlying this...


    pub_type: 杂志文章


    authors: Ceppo F,Berthou F,Jager J,Dumas K,Cormont M,Tanti JF

    更新日期:2014-03-01 00:00:00

  • Changes in adiponectin and inflammatory genes in response to hormonal imbalances in female mice and exacerbation of depot selective visceral adiposity by high-fat diet: implications for insulin resistance.

    abstract::Early obesity and late onset of insulin resistance associated with hormonal imbalances occur in FSH receptor-deficient follitropin receptor knockout female mice. This study tests the hypothesis that chronic high-fat diet aggravates obesogenic changes in a depot-specific manner and explores some molecular links of horm...


    pub_type: 杂志文章


    authors: Zhang H,Chen X,Aravindakshan J,Sairam MR

    更新日期:2007-12-01 00:00:00

  • Corticosterone concentrations in the serum and milk of lactating rats: parallel changes after induced stress.

    abstract::Transfer of corticosterone from serum to milk was studied in lactating rats after ether and pentobarbital anesthesia. Two minutes after the onset of anesthesia stress, total serum and milk corticosterone concentrations were not significantly different. By 30 min, corticosterone concentrations in serum increased to a p...


    pub_type: 杂志文章


    authors: Yeh KY

    更新日期:1984-10-01 00:00:00

  • Energetic metabolism and human sperm motility: impact of CB₁ receptor activation.

    abstract::It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role o...


    pub_type: 杂志文章


    authors: Barbonetti A,Vassallo MR,Fortunato D,Francavilla S,Maccarrone M,Francavilla F

    更新日期:2010-12-01 00:00:00

  • Ambient light modifies gonadotropin-releasing hormone pulse generator frequency in the rhesus monkey.

    abstract::In the course of previous studies using continuous monitoring of the electrophysiological correlates of GnRH pulse generator activity, characterized by episodic increases in hypothalamic multiunit electrical activity (MUA volley), it was noticed that the nocturnal slowing of pulse generator frequency was an acute phen...


    pub_type: 杂志文章


    authors: O'Byrne KT,Thalabard JC,Chiappini SE,Chen MD,Hotchkiss J,Knobil E

    更新日期:1993-10-01 00:00:00

  • Interleukin-6 is an essential determinant of on-time parturition in the mouse.

    abstract::IL-6 abundance in amniotic fluid and uterine tissues increases in late gestation or with infection-associated preterm labor. A role in regulation of labor onset is suggested by observations that IL-6 increases expression of genes controlling prostaglandin synthesis and signaling in isolated uterine cells, but whether ...


    pub_type: 杂志文章


    authors: Robertson SA,Christiaens I,Dorian CL,Zaragoza DB,Care AS,Banks AM,Olson DM

    更新日期:2010-08-01 00:00:00

  • Essential role of the oocyte in estrogen amplification of follicle-stimulating hormone signaling in granulosa cells.

    abstract::The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...


    pub_type: 杂志文章


    authors: Otsuka F,Moore RK,Wang X,Sharma S,Miyoshi T,Shimasaki S

    更新日期:2005-08-01 00:00:00

  • Progesterone-induced changes in hypothalamic luteinizing hormone-releasing hormone and catecholamines: differential effects of pentobarbital.

    abstract::The effects of pentobarbital (Pnt) treatment on the progesterone (P)-induced afternoon increase in the medial basal hypothalamic (MBH) LHRH and serum LH and FSH levels in ovariectomized estradiol benzoate-primed rats were studied. Pnt injection before P blocked the afternoon rise in serum gonadotropins but failed to a...


    pub_type: 杂志文章


    authors: Kalra SP,Simpkins JW,Kalra PS

    更新日期:1981-04-01 00:00:00

  • Strain-specific response to beta 3-adrenergic receptor agonist treatment of diet-induced obesity in mice.

    abstract::Fat intake has long been associated with the development of obesity. The studies described herein show that fat adversely affects adipocyte adrenergic receptor (AR) expression and function. As beta 3AR agonists have been shown to acutely reduce adipose tissue mass and improve thermogenesis in genetically obese rodents...


    pub_type: 杂志文章


    authors: Collins S,Daniel KW,Petro AE,Surwit RS

    更新日期:1997-01-01 00:00:00

  • Effects of thyrotropin and cholera toxin on the thyroidal adenylate cyclase-adenosine 3',5'-monophosphate system.

    abstract::The present experiments examined the relationship between cholera toxin and TSH stimulation of the adenylate cyclase system in bovine thyroid tissue. Preincubation of thyroid slices for 20 min at 4 C with a maximal concentration of cholera toxin (100 microgram/ml) did not impair the subsequent stimulation of cAMP by s...


    pub_type: 杂志文章


    authors: Holmes SD,Titus G,Chou M,Field JB

    更新日期:1980-12-01 00:00:00

  • Specific receptor-mediated inhibition by synthetic atrial natriuretic factor of hormone-stimulated steroidogenesis in cultured bovine adrenal cells.

    abstract::The effect of synthetic atrial natriuretic factor (ANF) on adrenal steroidogenesis has been studied in primary culture of bovine adrenal cells. ANF-(8-33) produced a potent 40-70% inhibition of angiotensin II-, ACTH-, PGE1-, and forskolin-stimulated secretion of aldosterone production from zona glomerulosa cells with ...


    pub_type: 杂志文章


    authors: De Léan A,Racz K,Gutkowska J,Nguyen TT,Cantin M,Genest J

    更新日期:1984-10-01 00:00:00

  • The pharmacokinetics of thyrotropin-releasing hormone (TRH) and deamido-TRH in the rat.

    abstract::The pharmacokinetics of TRH and its metabolite deamido-TRH (TRH-OH) were evaluated and compared. After a 60- to 90-min infusion of the appropriate peptide into rats, the MCR, half-life of disappearance (t 1/2), and volume of distribution were calculated. The MCR of TRH averaged 4 ml/min, whereas that of TRH-OH was 6.5...


    pub_type: 杂志文章


    authors: Safran M,Wu CF,Matys R,Alex S,Emerson CH

    更新日期:1984-09-01 00:00:00

  • Glucagon-like peptide-1-(7-36)amide and a rise in cyclic adenosine 3',5'-monophosphate increase cytosolic free Ca2+ in rat pancreatic beta-cells by enhancing Ca2+ channel activity.

    abstract::Glucagon-like peptide-1 (GLP-1), in the form of either GLP-1-(7-36)amide or GLP-1-(7-37), has been shown to potently stimulate insulin release in a glucose-dependent manner and is suggested to be a physiological incretin. To explore the mechanisms by which GLP-1-(7-36)amide stimulates insulin release, we investigated ...


    pub_type: 杂志文章


    authors: Yada T,Itoh K,Nakata M

    更新日期:1993-10-01 00:00:00

  • Release of oxytocin and vasopressin by intracerebroventricular vasoactive intestinal polypeptide.

    abstract::Vasoactive intestinal polypeptide (VIP)ergic nerves innervate both the neurohypophysis and the hypothalamus. To test the hypothesis that VIP is a releasing factor for neurohypophyseal hormones, rats were given intracerebroventricular (icv) infusions of VIP in doses varying from 0.3 pmol/kg.min to 3 nmol/kg.min for 5 m...


    pub_type: 杂志文章


    authors: Bardrum B,Ottesen B,Fahrenkrug J,Fuchs AR

    更新日期:1988-11-01 00:00:00

  • Up-regulation of vinculin expression in 3T3 preadipose cells by growth hormone.

    abstract::In an effort to define biochemical events relevant to the adipogenic action of GH, the effect of GH on expression of the cytoskeletal element vinculin was studied in 3T3-F442A preadipose cells. Results from Western blotting indicated that in serum-free medium 2 nM met-hGH induced an approximately 100% increase in vinc...


    pub_type: 杂志文章


    authors: Guller S,Corin RE,Yuan-Wu K,Sonenberg M

    更新日期:1991-07-01 00:00:00

  • Hormonal control of growth in the infant rat: further evidence that neither thyrotropin nor thyroid hormones are transferred via milk to suckling pups.

    abstract::Experiments were conducted to obtain information on the possible transfer of TSH and thyroid hormones from mother rats to their infants via milk. The mothers were thyroidectomized by surgical or chemical (tapazole) means to eliminate maternal thyroid hormones from milk while increasing its content of TSH. Their pups w...


    pub_type: 杂志文章


    authors: Glasscock GF,Nicoll CS

    更新日期:1983-03-01 00:00:00

  • Disrupted corticosterone pulsatile patterns attenuate responsiveness to glucocorticoid signaling in rat brain.

    abstract::Chronically elevated circulating glucocorticoid levels are although to enhance vulnerability to psychopathology. Here we hypothesized that such sustained glucocorticoid levels, disturbing corticosterone pulsatility, attenuate glucocorticoid receptor signaling and target gene responsiveness to an acute challenge in the...


    pub_type: 杂志文章


    authors: Sarabdjitsingh RA,Isenia S,Polman A,Mijalkovic J,Lachize S,Datson N,de Kloet ER,Meijer OC

    更新日期:2010-03-01 00:00:00

  • Morphological evidence for direct interaction between arcuate nucleus neuropeptide Y (NPY) neurons and gonadotropin-releasing hormone neurons and the possible involvement of NPY Y1 receptors.

    abstract::Neuropeptide Y (NPY) neurons in the arcuate nucleus of the hypothalamus (ARH) have been shown to play an important role in modulating LH secretion. One mechanism by which the ARH NPY system may regulate LH secretion is by modulating GnRH neuronal function. Thus, the present study examined whether the ARH NPY system pr...


    pub_type: 杂志文章


    authors: Li C,Chen P,Smith MS

    更新日期:1999-11-01 00:00:00

  • Colocalization of somatostatin receptor sst5 and insulin in rat pancreatic beta-cells.

    abstract::Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic delta-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenge...


    pub_type: 杂志文章


    authors: Mitra SW,Mezey E,Hunyady B,Chamberlain L,Hayes E,Foor F,Wang Y,Schonbrunn A,Schaeffer JM

    更新日期:1999-08-01 00:00:00

  • Gender-specific pattern of insulin-like growth factor-binding protein-3 protease activity in mouse thyroid.

    abstract::There is evidence that the IGF system plays an important role in the growth and function of the thyroid gland. Proteolysis is an important posttranslational process that modulates the affinity of IGF binding proteins (IGFBPs) to IGFs, thus regulating their activity. IGFBP-3 has been shown to be cleaved by members of t...


    pub_type: 杂志文章


    authors: Wang J,Grünler J,Lewitt MS

    更新日期:2004-03-01 00:00:00