Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish.


:Kisspeptins are new actors in the neuroendocrine regulation of reproduction. In vertebrates, the number of kiss genes varies from none to three. Zebrafish have two kiss genes, kiss1 and kiss2, and two kiss receptors (GPR54), kiss1r and kiss2r. To provide detailed information on the organization of the kiss systems in zebrafish, antibodies were raised against the C terminus of zebrafish preproKiss1 and preproKiss2. Immunohistochemistry fully confirmed in situ hybridization data, showing that kiss1-expressing neurons are only located in the habenular nucleus, while kiss2-expressing neurons are found in the dorsal and ventral hypothalamus. Kiss1-expressing cells project only to the interpeduncular and raphe nuclei and strongly expressed the kiss1r receptor. In contrast, kiss2-expressing cells are mostly present in the dorsal and ventral hypothalamus and project widely into the subpallium, the preoptic area, the thalamus, the ventral and caudal hypothalamus, and the mesencephalon. All these regions strongly expressed the kiss2r messengers. Kiss2 fibers profusely innervate the ventral forebrain and notably made close apposition with GnRH3 neurons. Estrogen treatment of juvenile fish with estradiol causes increase in kiss2 and kiss2r expression. In the pituitary gland, no proKiss2- positive fibers were detected, while positive cells were observed in the pars intermedia. In addition to proposing a successful strategy to develop antibodies to kisspeptins, these data indicate that the kiss2 systems of zebrafish are implicated in reproductive events, while the kiss1 gene would play other functions that remain to be established.






Servili A,Le Page Y,Leprince J,Caraty A,Escobar S,Parhar IS,Seong JY,Vaudry H,Kah O




Has Abstract


2011-04-01 00:00:00














  • Role of winged helix transcription factor (WIN) in the regulation of Sertoli cell differentiated functions: WIN acts as an early event gene for follicle-stimulating hormone.

    abstract::Members of the winged helix transcription factor family are known to regulate epithelial cell differentiation by regulating cell-specific gene expression. rWIN is a newly discovered member of the winged helix family shown to be present in the adult rat testis. In the testis the human homolog of rWIN, HFH-11, was local...


    pub_type: 杂志文章


    authors: Chaudhary J,Mosher R,Kim G,Skinner MK

    更新日期:2000-08-01 00:00:00

  • Dual expression of p80 type I and p68 type II interleukin-I receptors on anterior pituitary cells synthesizing growth hormone.

    abstract::Interleukin-1 alpha (IL-1 alpha) and IL-1 beta bind to either the p80 type I IL-1 receptor (IL-1RI) or the p68 type II IL-1R (IL-1RII) on both T and B lymphocytes. We and others have previously shown that the anterior pituitary gland also has specific high affinity binding sites for IL-1 alpha (Kd = approximately 1 nM...


    pub_type: 杂志文章,评审


    authors: French RA,Zachary JF,Dantzer R,Frawley LS,Chizzonite R,Parnet P,Kelley KW

    更新日期:1996-09-01 00:00:00

  • Leptin signaling targets the thyrotropin-releasing hormone gene promoter in vivo.

    abstract::The regulation of TRH gene expression in the paraventricular nucleus of the hypothalamus (PVH) by leptin is critical for normal function of the thyroid axis in rodents and humans. The TRH neuron in the PVH expresses both leptin and melanocortin-4 receptors, suggesting that both signaling systems may regulate TRH gene ...


    pub_type: 杂志文章


    authors: Guo F,Bakal K,Minokoshi Y,Hollenberg AN

    更新日期:2004-05-01 00:00:00

  • Characterization of thyrotropin-induced increase in iodothyronine monodeiodinating activity in mice.

    abstract::To further characterize the effect of TSH administration on thyroid iodothyronine monodeiodinating activity, we have evaluated the in vitro conversion of T4 to T3 (outer ring deiodination) and T3 to 3,3'-diiodothyronine (T2; inner ring deiodination) by mouse thyroid, liver, and kidney homogenates, comparing tissues fr...


    pub_type: 杂志文章


    authors: Wu SY,Reggio R,Florsheim WH

    更新日期:1985-03-01 00:00:00

  • Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures.

    abstract::Insulin-like growth factors I and II (IGF-I and -II) are polypeptides secreted by skeletal cells and are considered regulators of bone formation. IGF-I and -II were studied for their effects on collagen synthesis and degradation in cultures of intact fetal rat calvariae and on type I collagen transcript levels in oste...


    pub_type: 杂志文章


    authors: McCarthy TL,Centrella M,Canalis E

    更新日期:1989-01-01 00:00:00

  • N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.

    abstract::For successful human placentation, invasion of trophoblast cells into the uterus and its associated vasculature is essential, and the regulation of this process is controlled by many factors at the fetal-maternal interface. N-acetylglucosaminyltransferase V (GnT-V) is a key enzyme that catalyzes beta1, 6-N-acetylgluco...


    pub_type: 杂志文章


    authors: Yamamoto E,Ino K,Miyoshi E,Inamori K,Abe A,Sumigama S,Iwase A,Kajiyama H,Shibata K,Nawa A,Kikkawa F

    更新日期:2009-02-01 00:00:00

  • Estrogen acidifies vaginal pH by up-regulation of proton secretion via the apical membrane of vaginal-ectocervical epithelial cells.

    abstract::The objective of this study was to assess estrogen-dependent cellular mechanisms that could contribute to the acid pH of the vaginal lumen. Cultures of normal human cervical-vaginal epithelial (hECE) cells and endocervical cells were grown on filters, and acidification of the extracellular solutions on the luminal (L-...


    pub_type: 杂志文章


    authors: Gorodeski GI,Hopfer U,Liu CC,Margles E

    更新日期:2005-02-01 00:00:00

  • The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins.

    abstract::Mammary epithelial cells isolated from pregnant, nonlactating heifers were grown in vitro using collagen substrates. Using these systems, the truncated form of insulin growth factor-1 (IGF-1) (des-3-IGF-1), IGF-1, and IGF-2 all stimulated a significant (0.5 to 1 fold) increase in cell proliferation (des-3-IGF-1 greate...


    pub_type: 杂志文章


    authors: McGrath MF,Collier RJ,Clemmons DR,Busby WH,Sweeny CA,Krivi GG

    更新日期:1991-08-01 00:00:00

  • Synthesis of placental protein 12 by human endometrium.

    abstract::We have previously shown that placental protein 12 (PP12) is synthesized and secreted by human term pregnancy decidua in vitro. In the present study, fragments of proliferative and secretory phase endometrium were cultured in media in the presence and absence of progesterone (P) and 17 beta-estradiol (E2) for 96 h. Th...


    pub_type: 杂志文章


    authors: Rutanen EM,Koistinen R,Sjöberg J,Julkunen M,Wahlström T,Bohn H,Seppälä M

    更新日期:1986-03-01 00:00:00

  • Induction of thyroid-stimulating hormone receptor autoimmunity in hamsters.

    abstract::Female Chinese hamsters (n = 10) were immunized with Chinese hamster ovary (CHO) cells that expressed the human TSH receptor (TSHR) to generate a model of Graves' disease. TSHR-autoantibodies (TSHR-Ab) were determined by CHO-TSHR. Two hamsters with stimulating TSHR-Ab showed thyrocyte hypertrophy associated with a foc...


    pub_type: 杂志文章


    authors: Ando T,Imaizumi M,Graves P,Unger P,Davies TF

    更新日期:2003-02-01 00:00:00

  • p-Tyramine, a natural amine, inhibits prolactin release in vivo.

    abstract::p-Tyramine, an endogenous amine with sympathomimetic action, is found in the mammalian hypothalamus. When injected ip, p-tyramine reduced serum PRL without altering LH and TSH serum titers in adult orchidectomized rats and rats subjected to ether or immobilization stress. The hyperprolactinemia achieved by this last p...


    pub_type: 杂志文章


    authors: Becú-Villalobos D,Lacau de Mengido IM,Libertun C

    更新日期:1985-05-01 00:00:00

  • Repetitive activation of hypothalamic G protein-coupled receptor 54 with intravenous pulses of kisspeptin in the juvenile monkey (Macaca mulatta) elicits a sustained train of gonadotropin-releasing hormone discharges.

    abstract::The purpose of the present study was to further examine the hypothesis that activation of G protein-coupled receptor 54 (GPR54) signaling at the end of the juvenile phase of primate development is responsible for initiation of gonadarche and the onset of puberty. Accordingly, we determined whether repetitive iv admini...


    pub_type: 杂志文章


    authors: Plant TM,Ramaswamy S,Dipietro MJ

    更新日期:2006-02-01 00:00:00

  • Phenotypic plasticity of the ovarian surface epithelium: TGF-beta 1 induction of epithelial to mesenchymal transition (EMT) in vitro.

    abstract::Ovarian surface epithelium (OSE) is the most conceivable cell origin of epithelial ovarian carcinomas. Unlike many other epithelial tumors, the precancerous lesion acquires expression of epithelial markers, e.g. E-cadherin and claudins, suggesting that OSE cells undergo mesenchymal to epithelial transition (MET) durin...


    pub_type: 杂志文章


    authors: Zhu Y,Nilsson M,Sundfeldt K

    更新日期:2010-11-01 00:00:00

  • Reduced response of the hypothalamo-pituitary-adrenal axis to alpha1-agonist stimulation during lactation.

    abstract::To determine whether altered noradrenergic activation of the hypothalamo-pituitary-adrenal (HPA) axis contributes to the attenuated neuroendocrine response to stress observed during lactation, the effect of intracerebroventricular injection of the alpha1-agonist methoxamine (100 microg) was compared between virgin and...


    pub_type: 杂志文章


    authors: Windle RJ,Brady MM,Kunanandam T,Da Costa AP,Wilson BC,Harbuz M,Lightman SL,Ingram CD

    更新日期:1997-09-01 00:00:00

  • Complete adipose differentiation of 3T3 L1 cells in a chemically defined medium: comparison to serum-containing culture conditions.

    abstract::The hormonal control of the adipose conversion of 3T3 L1 cells was studied in a chemically defined medium and compared to that under serum-containing conditions. Addition of insulin to a chemically defined medium (Dulbecco's Modified Eagle's Medium-Ham's F-12, 1:1) resulted in a dose-dependent increase in glycero-3-ph...


    pub_type: 杂志文章


    authors: Hauner H

    更新日期:1990-08-01 00:00:00

  • Effect of starvation on the circadian adrenocortical rhythm in rats.

    abstract::The circadian rhythm of blood corticosterone was examined in rats whose feeding was restricted to a specific time of day. The peak of the corticosterone level was found just before feeding time regardless of whether, the rats were kept on a 12-h light, 12-h dark cycle or in constant light or were blinded. It took at l...


    pub_type: 杂志文章


    authors: Kato H,Saito M,Suda M

    更新日期:1980-03-01 00:00:00

  • Germ cell stimulation of Sertoli cell protein phosphorylation.

    abstract::Cultures of Sertoli cells were treated with freshly isolated, intact germ cells to determine if germ cells were capable of influencing Sertoli cell function. By using two-dimensional polyacrylamide gel electrophoresis and autoradiography, germ cells were found to increase several-fold the incorporation of [32P]orthoph...


    pub_type: 杂志文章


    authors: Ireland ME,Welsh MJ

    更新日期:1987-04-01 00:00:00

  • In vitro and in vivo calcitonin I gene expression in parenchymal cells: a novel product of human adipose tissue.

    abstract::Circulating levels of calcitonin precursors (CTpr), including procalcitonin (ProCT), increase up to several thousand-fold in human sepsis, and immunoneutralization improves survival in two animal models of this disease. Herein, we analyzed inflammation-mediated calcitonin I gene (CALC I) expression in human adipocyte ...


    pub_type: 杂志文章


    authors: Linscheid P,Seboek D,Nylen ES,Langer I,Schlatter M,Becker KL,Keller U,Müller B

    更新日期:2003-12-01 00:00:00

  • A regulatory loop between the hypothalamo-pituitary-adrenal (HPA) axis and circulating leptin: a physiological role of ACTH.

    abstract::The product of the ob/ob gene, leptin, is known to be able to exert a modulator, role on HPA axis function. The aim of the present study was to determine whether endogenous ACTH and glucocorticoids exert any regulatory effect on leptin secretion. For this purpose bilaterally adrenalectomized (ADX) or sham operated (Sh...


    pub_type: 杂志文章


    authors: Spinedi E,Gaillard RC

    更新日期:1998-09-01 00:00:00

  • Prolonged somatostatin pretreatment desensitizes somatostatin's inhibition of receptor-mediated release of adrenocorticotropin hormone and sensitizes adenylate cyclase.

    abstract::Addition of somatostatin-14 (SRIF) inhibits corticotropin releasing factor (CRF) and forskolin-stimulated cyclic AMP formation and ACTH release from tumor cells of the mouse anterior pituitary (AtT-20/D16-16). After long-term pretreatment of these cells with SRIF, the ability of SRIF to inhibit CRF and forskolin-stimu...


    pub_type: 杂志文章


    authors: Reisine T,Axelrod J

    更新日期:1983-08-01 00:00:00

  • Intravenous glucose administration in fasting rats has differential effects on acylated and unacylated ghrelin in the portal and systemic circulation: a comparison between portal and peripheral concentrations in anesthetized rats.

    abstract::Ghrelin is produced by the gastrointestinal tract, and its systemic concentrations are mainly regulated by nutritional factors. Our aim was to investigate: 1) endogenous portal and systemic acylated and unacylated ghrelin levels (AG and UAG, respectively); 2) whether an iv glucose tolerance test (IVGTT) modifies AG an...


    pub_type: 杂志文章


    authors: Gauna C,Uitterlinden P,Kramer P,Kiewiet RM,Janssen JA,Delhanty PJ,van Aken MO,Ghigo E,Hofland LJ,Themmen AP,van der Lely AJ

    更新日期:2007-11-01 00:00:00

  • Detection of the free beta subunit of human chorionic gonadotropin (HCG) in cultures of normal and malignant trophoblast cells, pregnancy sera, and sera of patients with choriocarcinoma.

    abstract::Immunoaffinity adsorption techniques, utilizing specific antisera for hCG and its subunits bound to Sepharose 4B, have been employed to separate hCG alpha beta dimer and free subunits of hCG. As previously reported by this and a number of other laboratories, trophoblast cells (in vivo and in vitro) produce free alpha ...


    pub_type: 杂志文章


    authors: Cole LA,Hartle RJ,Laferla JJ,Ruddon RW

    更新日期:1983-09-01 00:00:00

  • Roles of insulin receptor substrate-1 and Shc on insulin-like growth factor I receptor signaling in early passages of cultured human fibroblasts.

    abstract::Insulin-like growth factor-I (IGF-I) improves glucose metabolism and growth in patients with leprechaunism. We investigated signal transduction through IGF-I receptor in comparison with epidermal growth factor (EGF) receptor in early passages of cultured skin fibroblasts from a normal subject and a patient with leprec...


    pub_type: 杂志文章


    authors: Takahashi Y,Tobe K,Kadowaki H,Katsumata D,Fukushima Y,Yazaki Y,Akanuma Y,Kadowaki T

    更新日期:1997-02-01 00:00:00

  • Effects of human erythrocyte guanine nucleotide-binding regulatory protein on parathyroid hormone-responsive adenylate cyclase from canine renal cortex.

    abstract::We studied the effects of the guanine nucleotide-binding regulatory protein (Gs) from human erythrocytes on PTH-responsive adenylate cyclase from partially purified membranes of canine renal cortex (CRC). Extracts of erythrocyte membranes, containing soluble Gs, was obtained by treatment with a detergent (Lubrol PX). ...


    pub_type: 杂志文章


    authors: Levine MA,Greene A,Turner RT,Bell NH

    更新日期:1984-10-01 00:00:00

  • Transcriptional cofactors exhibit differential preference toward peroxisome proliferator-activated receptors alpha and delta in uterine cells.

    abstract::We previously showed that peroxisome proliferator-activated receptor delta (PPARdelta) is crucial for embryo implantation as a receptor for cyclooxygenase-2-derived prostacyclin in mice. PPARs belong to the nuclear receptor superfamily. They form heterodimer with a retinoid X receptor, recruit transcriptional cofactor...


    pub_type: 杂志文章


    authors: Lim HJ,Moon I,Han K

    更新日期:2004-06-01 00:00:00

  • NADPH-cytochrome P-450 reductase from untreated rat testicular and liver microsomes: isolation and comparison.

    abstract::NADPH-cytochrome P-450 reductase (EC, the enzyme involved in progesterone 17-hydroxylation, was purified to apparent homogeneity by detergent solubilization of the microsomal fractions of liver and testis from untreated rats. The enzymes from these two tissues were then compared with regard to several paramet...


    pub_type: 杂志文章


    authors: Hales DB,Betz G

    更新日期:1986-08-01 00:00:00

  • Ligand interaction at the estrogen receptor to program antiestrogen action: a study with nonsteroidal compounds in vitro.

    abstract::The estrogenic and antiestrogenic actions of the geometric isomers of tamoxifen and 4-hydroxytamoxifen were determined in a PRL synthesis assay using primary cultures of dispersed immature rat pituitary gland cells. 4-Hydroxytamoxifen was 100 times more potent as an antiestrogen than tamoxifen. The cis isomer of tamox...


    pub_type: 杂志文章


    authors: Jordan VC,Koch R,Langan S,McCague R

    更新日期:1988-04-01 00:00:00

  • GnRH-mediated desensitization of the pituitary gonadotrope is not calcium dependent.

    abstract::In the present work we describe homologous desensitization of gonadotropin release from pituitary cells in response to short-term exposure to GnRH. Like the release process itself, desensitization requires receptor occupancy by an agonist. In contrast, however, inhibition of LH release by chelation of extracellular ca...


    pub_type: 杂志文章


    authors: Smith WA,Conn PM

    更新日期:1983-01-01 00:00:00

  • Effect of restricted nutrition on puberty in the lamb: patterns of tonic luteinizing hormone (LH) secretion and competency of the LH surge system.

    abstract::The effects of undernutrition on the timing of puberty and regulation of LH secretion by the inhibitory and stimulatory feedback action of estradiol were examined in female sheep. The first experiment determined that maintenance of low BW (ca., 20 kg, four lambs) between 10 and 45 weeks of age prevented initiation of ...


    pub_type: 杂志文章


    authors: Foster DL,Olster DH

    更新日期:1985-01-01 00:00:00

  • Direct regulation of GnRH neuron excitability by arcuate nucleus POMC and NPY neuron neuropeptides in female mice.

    abstract::Hypothalamic neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons act to sense and coordinate the brain's responses to metabolic cues. One neuronal network that is very sensitive to metabolic status is that controlling fertility. In this study, we investigated the impact of neuropeptides released by NPY and POM...


    pub_type: 杂志文章


    authors: Roa J,Herbison AE

    更新日期:2012-11-01 00:00:00