Incorporation of inorganic [35S]-sulphate into glycoproteins of rat buccal and palatal minor salivary glands in vivo and in vitro.

Abstract:

:The incorporation of sodium [35S]-sulphate into the buccal and palatal minor glands of the albino rat was studied in vivo and in vitro. The biosynthesis of 35S-labelled macromolecules possessing the characteristics of sulphated glycoproteins was evident in both. Uptake of [35S]-sulphate into the palatal tissue in vivo was more rapid (approx. 10-fold at 2 h) and produced compounds of higher specific activity by comparison with buccal extracts. Following in-vitro culture in the presence of [35S]-sulphate, the 35S-labelled glycoproteins secreted into the medium contained a greater proportion of lower molecular-weight products and were of higher specific activity (in the range 10-20-fold greater) than those in the tissue fraction. No sulphated glycosaminoglycans were apparent in any of the products isolated.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Green DR,Embery G

doi

10.1016/0003-9969(84)90156-0

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

335-41

issue

5

eissn

0003-9969

issn

1879-1506

journal_volume

29

pub_type

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